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Data Absorbansi and Anthocyanin Concentration

This document contains absorbance data from samples measured at pH 1 and pH 4.5. It then shows calculations to determine the concentration of anthocyanins in the samples. The absorbance differences are used to calculate the anthocyanin concentration of each sample using the beer's law equation. The average anthocyanin concentration across all samples is then reported along with the standard deviation and coefficient of variation.

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54 views2 pages

Data Absorbansi and Anthocyanin Concentration

This document contains absorbance data from samples measured at pH 1 and pH 4.5. It then shows calculations to determine the concentration of anthocyanins in the samples. The absorbance differences are used to calculate the anthocyanin concentration of each sample using the beer's law equation. The average anthocyanin concentration across all samples is then reported along with the standard deviation and coefficient of variation.

Uploaded by

adeye
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Data Absorbansi :

pH 1

R1 R2 R3 R4 R5

λ510 nm = 1.020 1.007 1.034 1.026 1.029

λ700 nm = 0.011 0.021 0.022 0.013 0.017

pH 4.5 R1 R2 R3 R4 R5

λ510 nm = 0.343 0.344 0.329 0.341 0.337

λ700 nm = 0.081 0.089 0.077 0.086 0.079

Perhitungan :

A=A 510 −A 700 pH 1,0−( A 510− A 700 pH 4,5 )

A1 = 1.020 - 0.011 – 0.343-0.081

= 1.009 – 0.262

= 0.747

A2 = 1.007 – 0.021 – 0.344 – 0.089

= 0.986 – 0.255

= 0.731

A3 = 1.034 – 0.022 – 0.329 – 0.077

= 1.012 – 0.252

= 0.760

A4 = 1.026 – 0.013 – 0.341 – 0.286

= 1.013 – 0.255

= 0.758

A5 = 1.029 – 0.017 – 0.357 – 0.079

= 1.012 – 0.258
= 0.754

mg A x BM x FP x 1000
Konsentrasi antosianin ( )=
L ɛ xl

0.747 x 449.2 x 7 x 1000


A1 = = 87.318 mg/L
26900 x 1

0.731 x 449.2 x 7 x 1000


A2 = = 85.448 mg/L
26900 x 1

0.760 x 449.2 x 7 x 1000


A3 = = 88.838 mg/L
26900 x 1

0.758 x 449.2 x 7 x 1000


A4 = = 88. 604 mg/L
26900 x 1

0.754 x 449.2 x 7 x 1000


A5 = = 88.137 mg/L
26900 x 1

∑ = 87.669 mg/L

SD = √ ¿ ¿

√ 2.147
= = 1.465
4

1.465
CV = x 100 % = 1.662
88.132

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