Document PDF
Document PDF
a r t i c l e i n f o a b s t r a c t
Article history: The scarcity of freshwater has emerged as one of the most pressing problems of the 21st century. This
Received 27 December 2014 problem could be addressed partially by collection, treatment and reuse of greywater. In this context,
Received in revised form 5 June 2015 constructed wetlands (CWs) become attractive due to their simple mode of operation, effectiveness in
Accepted 9 June 2015
treatment and applicability at small/single household levels as well as in community levels. Hence the
present study investigates the treatment of greywater in a laboratory scale horizontal sub-surface flow
Keywords:
constructed wetland planted with Axonopus compressus (Sw.) P. Beauv. – a common landscape plant. The
Water reuse
experiment was conducted for a period of 50 days with change in wastewater every 10 days (1 run). During
Greywater treatment
Phytoremediation
each run the wastewater was circulated using a peristaltic pump at an HRT of 7.8 h. The results show
Constructed wetlands that CWs planted with A. compressus performed well in the treatment of greywater than the unplanted
Wastewater treatment control with an average removal of 93% turbidity, 95% COD, 98% NO3 − –N, 67% PO4 3− –P, and 95% of anionic
Rhizosphere treatment surfactants. The plant also survived well with increase in biomass and number during the experiment
and proved to be an efficient bioagent suitable for the treatment of greywater in CWs. The study further
suggests that the use of commercially valuable ornamental plants in the CWs as bioagents will improve
the aesthetic beauty, public acceptance and help to generate revenue apart from the major benefit of
wastewater treatment.
© 2015 Elsevier Ltd. All rights reserved.
[Link]
2214-7144/© 2015 Elsevier Ltd. All rights reserved.
154 V. Arunbabu et al. / Journal of Water Process Engineering 7 (2015) 153–160
Fig. 1. Schematic diagram of the subsurface flow constructed wetland with A. compressus plants.
as the inlet which distributed the greywater uniformly on the gravel greywater – mentioned as treated greywater in the rest of the text
bed. Another PVC pipe perforated at uniform intervals (10 cm) and – from each CW was drained out completely and untreated, fresh
fitted vertically at the downstream end of each CW served as the greywater was filled in each CW and the treatment was contin-
outlet (Fig. 1). Each of the constructed wetland was provided with ued. This 10 day operation is termed as one run. Thus the entire
a collection container (CC) in which the treated water exiting from experiment was consisted of 5 runs in other words during the total
CWs was collected (Fig. 2). experimental period of 50 days, the greywater replacement was
done 5 times of 10 days interval. However the plants in the planted
2.5. System operation CWs were allowed to remain for the entire period of 50 days.
For each CWs, 55 l of greywater was used (35 l in CWs and 20 l
The experiment was conducted in a subsurface flow (SSF) mode in the collection container). Flexible silicon tubes connected to the
in which the level of greywater was maintained just below the sur- automatic peristaltic pump delivered the greywater from the col-
face of the gravel bed. The CWs with and without plants were filled lection container to the respective constructed wetlands through
initially with tap water up to the sub-surface level and were circu- the inlet pipe at a flow rate of 75 ml/min (Fig. 3). Samples were
lated using a peristaltic pump (PP 20 Ex 4C, Miclins, India) for 25 collected from the outlet pipe on the 2nd, 5th, 7th and 10th days
days as a preconditioning step. After the preconditioning period of and analyzed for various parameters as per standard methods [23].
25 days the tapwater present in the CWs was completely drained On the eve of each sampling day, distilled water was added into
out from all the CWs. Now the experiment with greywater was each CW, up to the mark, in order to make-up the water loss due to
started by filling all the CWs with greywater and the treatment of evapo-transpiration. At the termination of the experiment, plants
greywater in the CWs was thus initiated. The greywater was circu- were removed from the CWs, washed thoroughly with tap water
lated in the CWs at a flow rate of 75 ml/min by using a peristaltic and then with distilled water, total number and mass (dry weight)
pump. This was continued for 10 days, at the end of 10th day the of the plants were also recorded.
Fig. 2. Experimental set-up showing the planted CWs. Fig. 3. Schematic layout plan of the experimental set up.
156 V. Arunbabu et al. / Journal of Water Process Engineering 7 (2015) 153–160
Table 2
Characteristics of synthetic greywater used for the experiment.
Parameters 1st Run 2nd Run 3rd Run 4th Run 5th Run Average
Table 3
Characteristics of the wastewater before and after treatment.
100 100
Planted Control
90 90
70 70
st st
60 1 run 60 1 run
nd nd
2 run 2 run
rd rd
50 3 run 50 3 run
th th
4 run 4 run
th th
40 5 run 40 5 run
30 30
2 4 6 8 10 2 4 6 8 10
Number of days Number of days
Fig. 4. Turbidity reduction (%) as recorded with the planted and control (without plants) CWs at different runs of the experiment.
100 100
Planted Control
90 90
Reduction in COD (%)
80 80
70 70
st st
1 run 1 run
60 nd 60 nd
2 run 2 run
rd rd
3 run 3 run
50 th
4 run 50 th
4 run
th th
5 run 5 run
40 40
2 4 6 8 10 2 4 6 8 10
Number of days Number of days
Fig. 5. COD reduction (%) as recorded with the planted and control (without plants) CWs at different runs of the experiment.
constructed wetlands. Vymazal and Kröpfelová [29] reviewed the 3.3. Nutrient removal
performance of more than 400 horizontal flow constructed wet-
lands from 36 countries treating different wastewater and reported The nutrient removal efficiencies of the CWs were assessed on
a positive effect of macrophytes on organics removal in most of the the basis of the percentage reduction of the nutrients from the
studies. Münch et al. [30] reported that plants in constructed wet- effluent coming out of the CWs. Among the two nutrients studied,
lands serve as carriers for attached microbial growth. Plants in CWs removal of NO3 − –N was better than PO4 3− –P removal in all the sys-
mainly transfer oxygen and release exudates through the roots into tems including the control. Better nutrient removals were observed
the root zone. As a consequence of this an area around the roots, with the planted CWs than the unplanted CW (control) in all the
called the rhizosphere, facilitate growth and survival of diverse runs of the experiment. Based on the average values the differ-
group of microorganisms by providing a conducive environment ence in NO3 − –N removal between the planted and unplanted CWs
with enriched oxygen content to the microorganisms. The plant rhi- ranged from 1.7% to 4.9% and the difference in PO4 3− –P removal
zosphere was reported to stimulate microbial community density ranged from 5.2% to 11.5%, respectively (Table 3).
and activity by providing root surface for microbial attachment and
growth, a source of organic content, nutrients and a micro-aerobic
3.3.1. Removal of NO3 − –N
environment via root oxygen release. The root zone or rhizosphere
The removal of nitrogen from the effluent was excellent and
is said to be the active reaction zone of constructed wetlands where
the CWs planted with A. compressus have performed with bet-
physicochemical and biological processes take place, induced by
ter removal of NO3 − –N in all the 5 runs of the experiment than
the interaction of plants, microorganisms, the soil and pollutants
the unplanted control. The NO3 − –N was reduced to 0.11 mg/l in
[31].
the planted CWs and 0.28 mg/l in the control by the end of the
158 V. Arunbabu et al. / Journal of Water Process Engineering 7 (2015) 153–160
100 100
Planted Control
95 95
90 90
2 4 6 8 10 2 4 6 8 10
Number of days Number of days
Fig. 6. NO3 − –N reduction (%) as recorded with the planted and control (without plants) CWs at different runs of the experiment.
treatment (Table 3). From the results it could be observed that 3.4. Anionic surfactants
the majority of the NO3 − –N removal occurred within the 2nd
day of each run in all the CWs (Fig. 6). The overall results of the In the present study anionic surfactant reduction was deter-
study shows that the systems planted with A. compressus removed mined from the 3rd run onwards (Fig. 8). Good removal of anionic
1.7–4.9% more NO3 − –N from the system than the control. The aver- surfactant was observed in each run and the majority of the sur-
age removal of NO3 − –N for the planted systems was 98% and for factant reduction was observed within the first 5 days of each run.
the control it was 94%. Nitrate-nitrogen can be reduced by den- CWs planted with A. compressus shows about an average of 95% of
itrification, plant uptake and conversion to bacteria cell [16,32]. surfactant removal whereas in the control, the removal of anionic
Prevailing anoxic and anaerobic conditions within the system offer surfactant was only 85%.
suitable conditions for denitrification in CWs. Degradation of surfactants through microbial activity is the
primary transformation occurring in the environment. Anionic sur-
factants are readily biodegradable under aerobic conditions as
opposed to anaerobic conditions, at which they are unlikely to be
3.3.2. Removal of PO4 3− –P degraded [36]. Leal et al. [37] observed 97% removal of anionic sur-
The constructed wetlands planted with A. compressus have per- factants in an aerobic greywater treatment system compared to
formed with better removal of PO4 3− –P in all the 5 runs of the 27% removal in an anaerobic treatment system. Chemical structure
experiment than the control. The peak removal of PO4 3− –P was and physio-chemical conditions of the environmental media are
observed within the first 5 days of the experiment (Fig. 7). The other factors which affect the biodegradation of surfactants in the
overall results of the study shows that the systems planted with environment [36].
A. compressus removed 5.2–11.5% more PO4 3− –P from the system
than the control. The maximum removal of PO4 3− –P was observed
in the 5th run of the experiment, in which the average removal for 3.5. Plant biomass and number
A. compressus was 75% and for the unplanted system (control) the
removal was 65%. The plants survived well during the experiment and showed an
Subsurface flow CWs has a greater potential to remove nitrogen increase in phytomass (53%) and number (17%) at the end of the
than phosphorus [33]. Brix et al. [34] reported that sorption of phos- experiment. Apart from the number and phytomass increase, sig-
phorus to the bed material was the major removal mechanism for nificant development of the root system was also noticed (Fig. 9).
phosphorus in subsurface flow CWs followed by incorporation into This indicates that A. compressus can be employed as a good bioa-
organisms (biofilms and plants). The phosphorus may be bound gent for the treatment of greywater in constructed wetlands.
to the substrate as a consequence of adsorption and precipitation
reactions with calcium, aluminium and iron in the gravel substrate. 3.7. Greywater treatment – future perspectives
The phosphorus removal efficiency of constructed wetlands can be
enhanced by incorporating a medium with high P-binding capac- One way to address the present day water crisis is to treat and
ity or by establishing a separate filter unit containing a material reuse greywater. The primary objective of greywater treatment
with a high P-binding capacity such as calcite [34,35]. The bet- and reuse is to reduce the demand on freshwater and to prevent
ter removal of phosphorus in the planted systems observed in water pollution due to the discharge of untreated wastewater. Use
the present study could be attributed to plant uptake and micro- of advanced treatment technologies are not sustainable in devel-
bial assimilation. The main role of plants with respect to removal oping countries as a result of high capital investment, requirement
of phosphorus is in the direct uptake and provision of suitable of consistent power supply and skilled labor, high operation and
conditions for microorganisms that use phosphorus as a nutrient maintenance costs. Hence it is better to promote simple and cost
[17]. effective technologies like constructed wetlands which can also
V. Arunbabu et al. / Journal of Water Process Engineering 7 (2015) 153–160 159
80 80
Planted Control
70 70
3-
50 50
40 40
st st
1 run 1 run
nd nd
30 2 run 30 2 run
rd rd
3 run 3 run
th th
4 run 4 run
20 th 20 th
5 run 5 run
2 4 6 8 10 2 4 6 8 10
Number of days Number of days
Fig. 7. PO4 3− –P reduction (%) as recorded with the planted and control (without plants) CWs at different runs of the experiment.
Planted Control
100 100
Reduction in Anionic Surfactants (%)
90 90
80 80
70 70
rd rd
3 run 3 run
60 th
4 run 60 th
4 run
th th
5 run 5 run
50 50
40 40
2 4 6 8 10 2 4 6 8 10
Number of days Number of days
Fig. 8. Anionic surfactant reduction (%) as recorded with the planted and control (without plants) wetlands at different runs of the experiment.
Fig. 9. A. Compressus plants before (a) and after (b) the greywater treatment in the CWs.
160 V. Arunbabu et al. / Journal of Water Process Engineering 7 (2015) 153–160
be operated as decentralized treatment facilities. Constructed wet- [10] F. Li, K. Wichmann, R. Otterpohl, Review of the technological approaches for
lands have been proved to be efficient in the removal of turbidity, grey water treatment and reuses, Sci. Total Environ. 407 (11) (2009)
3439–3449.
organic matter and pathogens, however the removal of nutrients [11] J. Vymazal, Constructed wetlands for wastewater treatment, Water 2 (2010)
and other emerging pollutants such as personal care products are 530–549.
a challenge and require more research. [12] C.G. Lee, T.D. Fletcher, G. Sun, Nitrogen removal in constructed wetland
systems, Eng. Life Sci. 9 (1) (2009) 11–22.
[13] G. Langergraber, K. Leroch, A. Pressl, K. Sleytr, R. Rohrhofer, R. Haberl,
4. Conclusions High-rate nitrogen removal in a two-stage subsurface vertical flow
constructed wetland, Desalination 246 (1) (2009) 55–68.
[14] A. Yalcuk, A. Ugurlu, Comparison of horizontal and vertical constructed
The overall results of the study indicate that the plant species (A. wetland systems for landfill leachate treatment, Bioresour. Technol. 100 (9)
compressus) tested in this study could survive well in the CWs. The (2009) 2521–2526.
plant has tolerated the influent wastewater and exhibited growth [15] L. Yang, H.T. Chang, M.N.L. Huang, Nutrient removal in gravel- and soil-based
wetland microcosms with and without vegetation, Ecol. Eng. 18 (2001)
in terms of increase in number and biomass, with comparable effi-
91–105.
ciency of greywater treatment. [16] T. Bindu, V.P. Sylas, M. Mahesh, P.S. Rakesh, E.V. Ramasamy, Pollutant removal
The performance of A. compressus in the CW system in terms from domestic wastewater with Taro (Colocasia esculenta) planted in a
subsurface flow system, Ecol. Eng. 33 (1) (2008) 68–82.
of COD and nutrient removal is encouraging and comparable with
[17] S.E. Mbuligwe, Comparative effectiveness of engineered wetland systems in
the reported values. However, a scaled-up study may be needed the treatment of anaerobically pre-treated domestic wastewater, Ecol. Eng. 23
for further confirmation of the present findings. As A. compressus (4) (2004) 269–284.
being a plant used in landscaping, its use in CWs will improve the [18] M.E. Kaseva, Performance of a sub-surface flow constructed wetland in
polishing pre-treated wastewater—a tropical case study, Water Res. 38 (3)
aesthetic nature of the treatment yard, further as this plant sur- (2004) 681–687.
vives well in the CWs its prolonged use in the field may reduce [19] C.S. Akratos, V.A. Tsihrintzis, Effect of temperature, HRT, vegetation and
the cost and labor involved in replacing the dead plants with new porous media on removal efficiency of pilot-scale horizontal subsurface flow
constructed wetlands, Ecol. Eng. 29 (2) (2007) 173–191.
ones, thus carpet grass can be used continuously rather sustainably, [20] B.H. Lee, M. Scholz, What is the role of Phragmites australis in experimental
then the greywater management with this kind of system becomes constructed wetland filters treating urban runoff, Ecol. Eng. 29 (1) (2007)
sustainable. 87–95.
[21] T.Y. Ling, K. Apun, S.R. Zainuddin, Performance of a pilot-scale biofilters and
constructed wetland with ornamental plants in greywater treatment, World
Acknowledgments Appl. Sci. J. 6 (11) (2009) 1555–1562.
[22] F. Hourlier, A. Masse, P. Jaouen, A. Lakel, C. Gerente, C. Faur, P. Le Cloirec,
Formulation of synthetic greywater as an evaluation tool for wastewater
Support from the Department of Science and Technology, Gov- recycling technologies, Environ. Technol. 31 (2) (2010) 215–223.
ernment of India, with DST-INSPIRE fellowship to Mr. Arun Babu [23] A.P.H.A. (American Public Health Association), Standard Methods for the
V and support from the University Grants Commission, Govern- Examination of Water and Wastewater, 20th ed., APHA, Washington, D.C,
1998.
ment of India, with the award of Faculty Improvement Programme
[24] Metcalf, Eddy. (2003). Wastewater Engineering: Treatment and Reuse. In: G.
(FIP) to Mr. Ignatius Antony have been gratefully acknowledged. Tchobanoglous, F.L. Burton, H.D. Stensel (Eds.). McGraw-Hill.
The authors also thank Department of Science and Technology, [25] J. Vymazal, Horizontal sub-surface flow and hybrid constructed wetlands
systems for wastewater treatment, Ecol. Eng. 25 (5) (2005) 478–490.
Government of India for the support through DST-FIST and PURSE
[26] C.C. Tanner, J.S. Clayton, M.P. Upsdell, Effect of loading rate and planting on
programmes. The authors gratefully acknowledge the anonymous treatment of dairy farm wastewaters in constructed wetlands—I. Removal of
reviewers for their valuable comments which helped a lot in oxygen demand, suspended solids and faecal coliforms, Water Res. 29 (1)
improving the manuscript. (1995) 17–26.
[27] M. Greenway, A. Woolley, Constructed wetlands in Queensland: performance
efficiency and nutrient bioaccumulation, Ecol. Eng. 12 (1) (1999) 39–55.
References [28] D. Steer, L. Fraser, J. Boddy, B. Seibert, Efficiency of small constructed wetlands
for subsurface treatment of single-family domestic effluent, Ecol. Eng. 18 (4)
[1] R. Kumar, R.D. Singh, K.D. Sharma, Water resources of India, Curr. Sci. 89 (5) (2002) 429–440.
(2005) 794–811. [29] J. Vymazal, L. Kröpfelová, Removal of organics in constructed wetlands with
[2] N.T.D. Trang, D. Konnerup, H.H. Schierup, N.H. Chiem, L.A. Tuan, H. Brix, horizontal sub-surface flow: a review of the field experience, Sci. Total
Kinetics of pollutant removal from domestic wastewater in a tropical Environ. 407 (13) (2009) 3911–3922.
horizontal subsurface flow constructed wetland system: effects of hydraulic [30] C. Münch, P. Kuschk, I. Rske, Root stimulated nitrogen removal: only a local
loading rate, Ecol. Eng. 36 (4) (2010) 527–535. effect or important for water treatment? Water Sci. Technol. 51 (9) (2005)
[3] A. Maimon, A. Tal, E. Friedler, A. Gross, Safe on-site reuse of greywater for 185–192.
irrigation-a critical review of current guidelines, Environ. Sci. Technol. 44 (9) [31] U. Stottmeister, A. Wießner, P. Kuschk, U. Kappelmeyer, M. Kästner, O.
(2010) 3213–3220. Bederski, R.A. Müller, H. Moormann, Effects of plants and microorganisms in
[4] A. Gross, D. Kaplan, K. Baker, Removal of chemical and microbiological constructed wetlands for wastewater treatment, Biotechnol. Adv. 22 (1)
contaminants from domestic greywater using a recycled vertical flow (2003) 93–117.
bioreactor (RVFB), Ecol. Eng. 31 (2) (2007) 107–114. [32] A. Al-Omari, M. Fayyad, Treatment of domestic wastewater by subsurface
[5] B. Jefferson, A. Palmer, P. Jeffrey, R. Stuetz, S. Judd, Grey water flow constructed wetlands in Jordan, Desalination 155 (1) (2003) 27–39.
characterisation and its impact on the selection and operation of technologies [33] J. Vymazal, Removal of nutrients in various types of constructed wetlands, Sci.
for urban reuse, Water Sci. Technol. 5 (2) (2004) 157–164. Total Environ. 380 (1) (2007) 48–65.
[6] R. Otterpohl, Options for alternative types of sewerage and treatment systems [34] H. Brix, C.A. Arias, M. Del Bubba, Media selection for sustainable phosphorus
directed to improvement of the overall performance, Water Sci. Technol. 45 removal in subsurface flow constructed wetlands, Water Sci. Technol. 44
(3) (2002) 149–158. (11–12) (2001) 47–54.
[7] E. Eriksson, K. Auffarth, M. Henze, A. Ledin, Characteristics of grey [35] C.A. Arias, H. Brix, N.H. Johansen, Phosphorus removal from municipal
wastewater, Urban Water 4 (1) (2002) 85–104. wastewater in an experimental two-stage vertical flow constructed wetland
[8] E. Eriksson, E. Donner, Metals in greywater: sources, presence and removal system equipped with a calcite filter, Water Sci. Technol. 48 (5) (2003) 51–58.
efficiencies, Desalination 248 (1) (2009) 271–278. [36] G.G. Ying, Fate, behavior and effects of surfactants and their degradation
[9] D. Konnerup, T. Koottatep, H. Brix, Treatment of domestic wastewater in products in the environment, Environ. Int. 32 (2002) 417–431.
tropical, subsurface flow constructed wetlands planted with Canna and [37] L.H. Leal, H. Temmink, G. Zeeman, C.J.N. Buisman, Comparison of three
Heliconia, Ecol. Eng. 35 (2) (2009) 248–257. systems for biological greywater treatment, Water 2 (2010) 155–169.