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Chitinase Bacillus sp. B26: Antifungal Agent

The study aimed to screen bacteria that produce chitinase enzymes and test their antifungal activity against plant pathogenic fungi. 83 bacteria isolates from marine samples were tested for chitinolytic activity, with 14 showing clear zones of chitin breakdown. Isolate B26 produced the most chitinase when grown in liquid media containing chitin. Crude chitinase from B26 restricted the growth of Fusarium solani by 44.55%. Bacillus sp. B26 was found to have potent antifungal effects and could be developed as a biocontrol agent against phytopathogenic fungi, providing an alternative to chemical fungicides.

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4 views1 page

Chitinase Bacillus sp. B26: Antifungal Agent

The study aimed to screen bacteria that produce chitinase enzymes and test their antifungal activity against plant pathogenic fungi. 83 bacteria isolates from marine samples were tested for chitinolytic activity, with 14 showing clear zones of chitin breakdown. Isolate B26 produced the most chitinase when grown in liquid media containing chitin. Crude chitinase from B26 restricted the growth of Fusarium solani by 44.55%. Bacillus sp. B26 was found to have potent antifungal effects and could be developed as a biocontrol agent against phytopathogenic fungi, providing an alternative to chemical fungicides.

Uploaded by

edy icp
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Screening of chitinase producing Bacillus sp.

and antifungal activity against


phytopathogenic fungi

Abstract

Objective: The objective of the present study was aimed to screen of chitinase
producing bacteria and antifungal activity against phytopathogenic fungi.

Methods: Bacteria isolates from marine samples were obtained from the Microbiology
Lab, Science Department, Science and Technology Faculty, Prince of Songkla
University. Bacteria isolates grown by streak plate technique on nutrient agar. All the
bacterial isolates were tested for chitinase activity using colloidal chitin agar media by
spot inoculation method and the potential isolate was used for chitinase production by
submerged fermentation technique using 1% colloidal chitin in the liquid medium. The
efficacy of chitinase activity was assessed by 3,5-dinitrosalicylic acid (DNS) method
along with growth monitoring. The most potential isolate was identified based on
morphological, biochemical, and physiological tests. The most potential Bacillus sp.
B26 isolate was evaluated for its antagonistic activity of crude chitinase against
phytopathogenic fungi namely Aspergillus flavus, Aspergillus niger, Fusarium solani,
and Penicillium chrysogenium used well diffusion method.

Results: Totally, 83 bacteria isolates from marine samples were screened for chitinolytic
activity. Based on the chitinolytic index, 14 isolates showed clear zone hydrolysis on
solid medium. Among the chitinase activity in submerged fermentation, the isolate B26
had prominent chitinase production after 5 days of incubation. The antagonistic activity
of the isolate B26 was effective in restricting the mycelium growth of Fusarium solani
by 44.55 %.

Conclusion: Bacillus sp. B26 has found to possess potent antifungal activity and it can
be used as a promising biocontrol agent against phytopathogenic fungi, thereby
reducing the use of chemical fungicides.

Keyword: chitinase, colloidal chitin, Bacillus sp. B26, biocontrol, phytopathogenic


fungi

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