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CD36 Transmembrane Protein Analysis

This document analyzes the transmembrane protein CD36 in the Macaca mulatta species using various bioinformatics tools. It provides the amino acid sequence of CD36 and analyzes it using the Kyte & Doolittle hydropathy scale. It also uses TMHMM to predict there are two transmembrane helices in residues 7-29 and 441-463 with the rest of the protein existing outside or inside the membrane.

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Christine Alayon
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6 views2 pages

CD36 Transmembrane Protein Analysis

This document analyzes the transmembrane protein CD36 in the Macaca mulatta species using various bioinformatics tools. It provides the amino acid sequence of CD36 and analyzes it using the Kyte & Doolittle hydropathy scale. It also uses TMHMM to predict there are two transmembrane helices in residues 7-29 and 441-463 with the rest of the protein existing outside or inside the membrane.

Uploaded by

Christine Alayon
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Kho, Nemuel DC.

Alayon, Christine P.

TRANSMEMBRANE PROTEIN: CD36 (Macaca mulatta)


Cell and Molecular Biology
BS Biology 4-1

ProtScale

User-provided sequence:
10 20 30 40 50 60
MGCDRNCGLI TGAVIGAVLA VFGGILMPVG DMLIQKTIKK EVVLEEGTIA FKNWVKTGTE

70 80 90 100 110 120


IYRQFWIFDV QNPQEVMMNS SNIQVKQRGP YTYRVRFLAK ENITQDPKDN TVSFLQPNGA

130 140 150 160 170 180


IFEPSLSVGT EADNFTVLNL AVAAASHIYP NPFVQVVLNS LINKSKSSMF QVRTLRELLW

190 200 210 220 230 240


GYTDPFLSLV PYPVSTRVGM FYPYNNTADG VYKVFNGKDS ISKVAIIDTY KGKRNLSYWE

250 260 270 280 290 300


SYCDMINGTD AASFPPFVEK SQVLQFFSSD ICRSIYAVFE SDVNLKGIPV YRFVLPSKAF

310 320 330 340 350 360


ASPVQNPDNH CFCTEKIISK NCTSYGVLDI SKCKEGKPVY ISLPHFLYAS PDVSETIDGL

370 380 390 400 410 420


NPNEEEHRTY LDIEPITGFT LQFAKRLQVN LLVKPSNKIQ VLKRLKRNYI VPILWLNETG

430 440 450 460 470


TIGDEKAKMF RSQVTGKINL LGLIEMILLS VGVVMFVAFM ISYCACRSKT IK
SEQUENCE LENGTH: 472

Using the scale Hphob. / Kyte & Doolittle, the individual values for the 20
amino acids are:
Ala: 1.800 Arg: -4.500 Asn: -3.500 Asp: -3.500 Cys: 2.500 Gln: -3.500
Glu: -3.500 Gly: -0.400 His: -3.200 Ile: 4.500 Leu: 3.800 Lys: -3.900
Met: 1.900 Phe: 2.800 Pro: -1.600 Ser: -0.800 Thr: -0.700 Trp: -0.900
Tyr: -1.300 Val: 4.200 : -3.500 : -3.500 : -0.490

Weights for window positions 1,..,9, using linear weight variation model:
1 2 3 4 5 6 7 8 9
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
edge center edge
TMHMM result
# WEBSEQUENCE Length: 472
# WEBSEQUENCE Number of predicted TMHs: 2
# WEBSEQUENCE Exp number of AAs in TMHs: 45.12088
# WEBSEQUENCE Exp number, first 60 AAs: 22.53542
# WEBSEQUENCE Total prob of N-in: 0.99500
# WEBSEQUENCE POSSIBLE N-term signal sequence
WEBSEQUENCE TMHMM2.0 inside 1 6
WEBSEQUENCE TMHMM2.0 TMhelix 7 29
WEBSEQUENCE TMHMM2.0 outside 30 440
WEBSEQUENCE TMHMM2.0 TMhelix 441 463
WEBSEQUENCE TMHMM2.0 inside 464 472

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