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Chemostat Study of Yeast Growth Rates

1. A chemostat study was done with yeast to determine kinetic parameters. Analysis of the data yielded Michaelis-Menten kinetics with a maximum rate (Vmax) of 0.481 g/L/hr and half-saturation constant (Km) of 0.268 g/L. The rate equation for cell growth was derived. 2. Another chemostat study with yeast varied the dilution rate and measured cell and substrate concentrations. Analysis of the data determined kinetic parameters of Vmax = 0.263 g/L/hr and Km = 1.63 g/L. The rate equation for cell growth was derived. The minimum flow rate to prevent washout was calculated to be 0.13 L

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Princess Janine Catral
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13 views3 pages

Chemostat Study of Yeast Growth Rates

1. A chemostat study was done with yeast to determine kinetic parameters. Analysis of the data yielded Michaelis-Menten kinetics with a maximum rate (Vmax) of 0.481 g/L/hr and half-saturation constant (Km) of 0.268 g/L. The rate equation for cell growth was derived. 2. Another chemostat study with yeast varied the dilution rate and measured cell and substrate concentrations. Analysis of the data determined kinetic parameters of Vmax = 0.263 g/L/hr and Km = 1.63 g/L. The rate equation for cell growth was derived. The minimum flow rate to prevent washout was calculated to be 0.13 L

Uploaded by

Princess Janine Catral
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Princess Janine B.

Catral BSChE IV

1. Aiba reported the results of a chemostat study on the growth of a specific strain of baker’s yeast. The inlet
streams of the chemostat did not contain any cells or products. Det. a) Km and Vmax, b) rate eqn for cell growth
c) rate eqn for ethanol production.

Dilution Rate (hr- Inlet Steady State


1
) Concentration Concentration (g/L) Column1 Column2 1/D 1/Cs
Glucose (g/L) Glucose(Cs) Ethanol(Cp) Cells(Cx) L/g L/g
0.084 21.5 0.054 7.97 2 11.90476 18.51852
0.1 10.9 0.079 4.7 1.2 10 12.65823
0.16 21.2 0.138 8.57 2.4 6.25 7.246377
0.198 20.7 0.186 8.44 2.33 5.050505 5.376344
0.242 10.8 0.226 4.51 1.25 4.132231 4.424779

A Plot of 1/D vs 1/Cs


16
y = 0.5585x + 2.081
14
R² = 0.9734
12
10
1/D

8
6
4
2
0
-5 0 5 10 15 20 25
1/Cs

a. From graph:
1/Vmax = 2.081
Vmax= 0.481
Km = 0.558Vmax = 0.558(0.481)
Km = 0.268

b. Rate equation for cell growth

Vmax Cs Cx
rx =
K m + Cs

0.481 Cs Cx
rx =
0.268 + Cs
2. A chemostat study was done with yeast. The medium flow rate was varied and the steady state concentration
of cells and glucose in the fermenter were measured and recorded. The inlet concentration of glucose was
determined at 100g/L. The volume of the fermenter contents was 500mL and the inlet stream was sterile. Find
the rate equation for cell growth. What should be the range of flow rate to prevent washout of the cells?

Cell Substrate D=
Flow rate concentration concentration F/Volume 1/D 1/Cs
F, L/hr Cx, g/L Cs, g/L /hr hr L/g
31 5.97 0.5 0.062 16.12903226 2
50 5.94 1 0.1 10 1
71 5.88 2 0.142 7.042253521 0.5
91 5.76 4 0.182 5.494505495 0.25
200 0 100 0.4 2.5 0.01

The plot of 1/D versus 1/Cs


25

20 y = 6.1909x + 3.8
R² = 0.9823
15
1/D

10

0
-1 -0.5 0 0.5 1 1.5 2 2.5 3
1/Cs

1/Vmax 3.8
Km/Vmax 6.19
Km 1.628947368
Vmax 0.263157895
rate equation, rx 0.26CsCx/1.63 + Cs
F 0.12946995
b. Rate equation and Flow rate

Vmax Cs Cx
rx =
K m + Cs

0.263 Cs Cx
rx =
1.63 + Cs
Volume(Csi)Vmax
F=
Km + Csi
0.5(100)(0.26)
= 1.63+100

F = 0.13 L/hr

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