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Monod Kinetics in Ethanol Fermentation

1. The document contains graphs and data tables showing Monod kinetics experiments with varying substrate concentrations. Specific growth rates of microbes decrease as substrate concentration decreases in a characteristic curve. 2. Graphs and equations show kinetics follow first order behavior with respect to substrate concentration. A linear relationship is seen between the log of substrate concentration and time. 3. Data is provided on fermentation kinetics including utilization of sugar, production of ethanol, and depletion of substrate over time. The kinetics follow zero order, first order, and polynomial behaviors depending on conditions.

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0% found this document useful (0 votes)
20 views16 pages

Monod Kinetics in Ethanol Fermentation

1. The document contains graphs and data tables showing Monod kinetics experiments with varying substrate concentrations. Specific growth rates of microbes decrease as substrate concentration decreases in a characteristic curve. 2. Graphs and equations show kinetics follow first order behavior with respect to substrate concentration. A linear relationship is seen between the log of substrate concentration and time. 3. Data is provided on fermentation kinetics including utilization of sugar, production of ethanol, and depletion of substrate over time. The kinetics follow zero order, first order, and polynomial behaviors depending on conditions.

Uploaded by

Kenu Abad
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as XLSX, PDF, TXT or read online on Scribd

Monod Kinetics

0.7

0.6
Specifc Growth Rate U per hr

0.5

0.4

0.3

0.2

0.1

0
0 20 40 60 80 100 120 140 160

Substrate Concentration g/L


frst order
4.000
f(x) = - 0.1287780833x + 3.9161145872
R = 0.9478201673

3.500

3.000

2.500
Axis Title

2.000 frst order


Linea r (frst order)

1.500

1.000

0.500

0.000
0 5 10 15 20 25 30

Axis Title
Fermentation of Sugar Kinetics
200.000

180.000

160.000

140.000

120.000

100.000

80.000

60.000

40.000

20.000

0.000
0.000 20.000 40.000 60.000 80.000 100.000 120.000 140.000
production of ethanol
14

f(x) = 0.5705523684x - 0.1158907359


R = 0.9338826395

12
f(x) = 1.21632678569439E-05x^5 - 0.0006477968x^4 + 0.0085554041x^3 + 0.0163798463x^2 + 0.083473714x + 0.0148101753

10

8
Column R
Axis Title

Li nea r (Column R)
Polynomia l (Col umn R)
6

0
0 5 10 15 20 25 30

Axis Title
Chart Title
200.000

180.000

160.000

140.000

120.000

100.000

80.000

60.000

40.000

20.000

0.000
0.000 5.000 10.000 15.000 20.000 25.000 30.000
Zeroth Order
40.000

35.000

30.000

25.000
Axis Title

20.000 Zeroth Order

15.000

10.000

5.000

0.000
0 5 10 15 20 25 30

Axis Title
Umax 0.65 per hour
Ks 11.39 g/L
Percent Sugar utilization 95.7

Substrate (g/L) U (per hour)


150 0.6041266497 time (h)
149 0.6038406384 0.0224245927
148 0.6035510383 4.0362672618
147 0.6032577814 7.9981667098
146 0.602960798 12.0083162293
145 0.6026600166 16.0475061276
144 0.6023553639 20.0367455114
143 0.6020467647 24.0522089154
142 0.6017341417
141 0.6014174158
140 0.6010965057
139 0.6007713279
138 0.6004417966
137 0.600107824
136 0.5997693195
135 0.5994261903
134 0.599078341
133 0.5987256735
132 0.598368087
131 0.5980054779
130 0.5976377396
129 0.5972647624
128 0.5968864337
127 0.5965026375
126 0.5961132542
125 0.5957181612
124 0.5953172317
123 0.5949103356
122 0.5944973386
121 0.5940781026
120 0.593652485
119 0.593220339
118 0.5927815133
117 0.5923358517
116 0.5918831933
115 0.5914233721
114 0.5909562166
113 0.59048155
112 0.5899991896
111 0.5895089468
110 0.5890106269
109 0.5885040286
108 0.5879889438
107 0.5874651575
106 0.5869324474
105 0.5863905834
104 0.5858393275
103 0.5852784334
102 0.5847076462
101 0.5841267017
100 0.5835353263
99 0.5829332367
98 0.582320139
97 0.5816957284
96 0.581059689
95 0.5804116928
94 0.5797513996
93 0.5790784558
92 0.5783924944
91 0.5776931341
90 0.5769799783
89 0.5762526148
88 0.5755106147
87 0.5747535319
86 0.5739809015
85 0.5731922399
84 0.5723870427
83 0.5715647844
82 0.570724917
81 0.5698668687
80 0.5689900427
79 0.5680938157
78 0.5671775366
77 0.5662405249
76 0.5652820689
75 0.5643014238
74 0.56329781
73 0.5622704112
72 0.5612183715
71 0.5601407938
70 0.5590367367
69 0.5579052121
68 0.556745182
67 0.5555555556
66 0.5543351854
65 0.5530828642
64 0.5517973206
63 0.5504772147
62 0.5491211337
61 0.5477275867
60 0.5462949993
59 0.5448217076
58 0.5433059519
57 0.5417458693
56 0.5401394866
55 0.5384847116
54 0.5367793241
53 0.535020966
52 0.5332071305
51 0.5313351499
50 0.5294021828
49 0.5274051995
48 0.5253409665
47 0.5232060284
46 0.5209966893
45 0.518708991
44 0.5163386893
43 0.5138812282
42 0.5113317101
41 0.5086848635
40 0.5059350068
39 0.5030760071
38 0.5001012351
37 0.4970035131
36 0.493775058
35 0.4904074154
34 0.4868913858
33 0.4832169408
32 0.4793731274
31 0.4753479594
30 0.4711282919
29 0.4666996781
28 0.4620462046
27 0.4571502996
26 0.4519925114
25 0.4465512503
24 0.4408024866
23 0.4347193952
22 0.4282719377
21 0.4214263662
20 0.414144632
19 0.4063836788
18 0.39809459
17 0.3892215569
16 0.3797006207
15 0.3694581281
14 0.3584088224
13 0.3464534645
12 0.3334758444
11 0.3193389906
10 0.3038803179
9 0.2869053458
8 0.268179474
7 0.2474170745
6 0.22426682
5 0.1982916412
4 0.1689408707
3 0.1355107714
2 0.0970873786
1 0.0524616626
0 0
frst order
Substrate Conc. g/L ln substrate t
35.015576324 3.556 0.02242459
29.989615784 3.401 4.03626726
23.759086189 3.168 7.99816671
12.9595015576 2.562 12.0083162
7.559709242 2.023 16.0475061
4.0706126687 1.404 20.0367455
1.5784008307 0.456 24.0522089

zeroth order fermentation


y = -1.5011x + 34.475 y = -4E-05x5 + 0.0022x4 - 0.0342x3 + 0.1229x2 - 1.2425x +
Substrate time (h)
187.539 1.000 187.888 0.571
186.038 2.000 186.807 1.141
184.537 3.000 185.661 1.712
183.036 4.000 184.360 2.282
181.535 5.000 182.844 2.853
180.033 6.000 181.085 3.424
178.532 7.000 179.076 3.994
177.031 8.000 176.828 4.565
175.530 9.000 174.362 5.135
174.029 10.000 171.705 5.706
172.528 11.000 168.881 6.277
171.027 12.000 165.908 6.847
169.526 13.000 162.790 7.418
168.025 14.000 159.513 7.988
166.524 15.000 156.036 8.559
165.022 16.000 152.290 9.130
163.521 17.000 148.164 9.700
162.020 18.000 143.509 10.271
160.519 19.000 138.123 10.841
159.018 20.000 131.750 11.412
157.517 21.000 124.073 11.983
156.016 22.000 114.709 12.553
154.515 23.000 103.198 13.124
153.014 24.000 89.006 13.694
151.513 25.000 71.509 14.265
150.011 26.000 49.995 14.836
148.510 27.000 23.653 15.406
147.009 28.000 -8.430 15.977
145.508 29.000 -47.277 16.547
144.007 30.000 -94.025 17.118
142.506 31.000 -149.934 17.689
141.005 32.000 -216.390 18.259
139.504 33.000 -294.913 18.830
138.003 34.000 -387.161 19.400
136.502 35.000 -494.939 19.971
135.000 36.000 -620.200 20.542
133.499 37.000 -765.059 21.112
131.998 38.000 -931.789 21.683
130.497 39.000 -1122.836 22.253
128.996 40.000 -1340.820 22.824
127.495 41.000 -1588.542 23.395
125.994 42.000 -1868.989 23.965
124.493 43.000 -2185.345 24.536
122.992 44.000 -2540.988 25.106
121.491 45.000 -2939.506 25.677
119.989 46.000 -3384.695 26.248
118.488 47.000 -3880.570 26.818
116.987 48.000 -4431.368 27.389
115.486 49.000 -5041.556 27.959
113.985 50.000 -5715.835 28.530
112.484 51.000 -6459.149 29.101
110.983 52.000 -7276.688 29.671
109.482 53.000 -8173.896 30.242
107.981 54.000 -9156.475 30.812
106.480 55.000 -10230.394 31.383
104.978 56.000 -11401.890 31.954
103.477 57.000 -12677.482 32.524
101.976 58.000 -14063.967 33.095
100.475 59.000 -15568.435 33.665
98.974 60.000 -17198.270 34.236
97.473 61.000 -18961.157 34.807
95.972 62.000 -20865.087 35.377
94.471 63.000 -22918.368 35.948
92.970 64.000 -25129.622 36.518
91.469 65.000 -27507.801 37.089
89.967 66.000 -30062.185 37.660
88.466 67.000 -32802.393 38.230
86.965 68.000 -35738.386 38.801
85.464 69.000 -38880.475 39.371
83.963 70.000 -42239.325 39.942
82.462 71.000 -45825.964 40.513
80.961 72.000 -49651.786 41.083
79.460 73.000 -53728.559 41.654
77.959 74.000 -58068.429 42.224
76.458 75.000 -62683.929 42.795
74.956 76.000 -67587.980 43.366
73.455 77.000 -72793.905 43.936
71.954 78.000 -78315.425 44.507
70.453 79.000 -84166.674 45.077
68.952 80.000 -90362.200 45.648
67.451 81.000 -96916.972 46.219
65.950 82.000 -103846.385 46.789
64.449 83.000 -111166.271 47.360
62.948 84.000 -118892.896 47.930
61.447 85.000 -127042.976 48.501
59.945 86.000 -135633.675 49.072
58.444 87.000 -144682.616 49.642
56.943 88.000 -154207.884 50.213
55.442 89.000 -164228.034 50.783
53.941 90.000 -174762.095 51.354
52.440 91.000 -185829.579 51.925
50.939 92.000 -197450.484 52.495
49.438 93.000 -209645.302 53.066
47.937 94.000 -222435.023 53.636
46.436 95.000 -235841.144 54.207
44.934 96.000 -249885.670 54.778
43.433 97.000 -264591.128 55.348
41.932 98.000 -279980.563 55.919
40.431 99.000 -296077.553 56.489
38.930 100.000 -312906.210 57.060
37.429 101.000 -330491.187 57.631
35.928 102.000 -348857.683 58.201
34.427 103.000 -368031.454 58.772
32.926 104.000 -388038.810 59.342
31.425 105.000 -408906.631 59.913
29.923 106.000 -430662.365 60.484
28.422 107.000 -453334.039 61.054
26.921 108.000 -476950.262 61.625
25.420 109.000 -501540.233 62.195
23.919 110.000 -527133.745 62.766
22.418 111.000 -553761.194 63.337
20.917 112.000 -581453.582 63.907
19.416 113.000 -610242.525 64.478
17.915 114.000 -640160.257 65.048
16.414 115.000 -671239.639 65.619
14.912 116.000 -703514.160 66.190
13.411 117.000 -737017.951 66.760
11.910 118.000 -771785.781 67.331
10.409 119.000 -807853.072 67.901
8.908 120.000 -845255.900 68.472
8.758 120.100 -849071.046 68.529
8.608 120.200 -852899.951 68.586
8.458 120.300 -856742.653 68.643
8.308 120.400 -860599.188 68.700
8.157 120.500 -864469.595 68.757
zeroth order
substrate time time ethanol frst order
35.016 0.0224245927 0.001819 0.0203353982 0.020335
29.990 4.0362672618 3.990362 0.9683288333 0.968329
23.759 7.9981667098 8.008683 3.9451809718 3.945181
12.960 12.0083162293 11.9495 7.5984456908 7.598446
7.560 16.0475061276 16.04723 10.9913680372 10.99137
4.071 20.0367455114 20.00952 11.9220583795 11.92206
1.578 24.0522089154 23.96971 11.6562199533 11.65622

.0342x3 + 0.1229x2 - 1.2425x + 34.996


0.001819
3.990362
8.008683
11.9495
16.04723
20.00952
23.96971

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