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Hybrid Fish Breeding Methodology Guide

The document outlines the methodology for collecting and breeding juvenile fish specimens from Lake Taal in the Philippines to produce hybrids. Live juvenile fish will be obtained from the lake or local market and raised to sexual maturity under controlled conditions outside of normal spawning season. Mature females will be injected with hormones like HCG or Nile tilapia pituitaries to induce spawning. Eggs will be stripped from females and fertilized with sperm before incubating in tanks.

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50 views2 pages

Hybrid Fish Breeding Methodology Guide

The document outlines the methodology for collecting and breeding juvenile fish specimens from Lake Taal in the Philippines to produce hybrids. Live juvenile fish will be obtained from the lake or local market and raised to sexual maturity under controlled conditions outside of normal spawning season. Mature females will be injected with hormones like HCG or Nile tilapia pituitaries to induce spawning. Eggs will be stripped from females and fertilized with sperm before incubating in tanks.

Uploaded by

Nicole
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Methodology

Juvenile male and female specimens of wild Oreochromis niloticus, Parachromis

managuensis, Sarotherodon melanotheron and Cichlasoma citrinellum were collected

via random sampling from Lake Taal, Batangas, Philippines. Due to possible

complications from not being able to collect adequate specimens, live juvenile

specimens may be bought from the local market instead. Collected specimens will be

raised to sexual maturity in a controlled environment. The fishes will be obtained during

a time outside of normal spawning season to produce hybrids with other species. Once

specimens have reached sexual maturity, mature females are injected with hormones in

order to provoke the mating and spawning processes. Hormones that will be used are

either HCG (Human Chorionic Gonadotropin), 25 I.U. per 100 g of female or pituitaries

of the Nile tilapia (Oreochromis niloticus), 3-4 pituitaries of a Nile tilapia (100-150 g) per

female catfish will induce ovulation. The required quantity of powdered acetone dried

pituitary material or the required number of whole pituitaries are pulverised in a

porcelain mortar, mixed with the required quantity of physiological salt solution (9 g of

common salt/litre of water). The pituitaries must be pulverized properly to avoid

complications while injecting the fishes. A syringe is filled with the suspension and the

injection can be given, 1 ml per fish. The container of the fishes will be covered in a

layer of stony gravel and temperature will be maintained between 20C to 30C in order

to mimic the natural environment parameters that Lake Taal possesses. Spawning

usually takes place during the night after the hormone treatment and the breeders then

removed the following morning. The most common method of administering the
hormone solution is by intra-muscular injection into the dorsal muscle. The head of the

fish can be covered with a wet towel during injection. When eyes are obstructed or

covered, fish tend to keep still. This ensures the fish is docile during injection of the

hormones. Stripping of the female spawners is carried out by gently pressing their

abdomen with a thumb from the pectoral fin towards the genital papilla. Ovulated eggs

will flow out easily in a thick jet from the genital vent and are usually collected into a dry

plastic container. The sperm (diluted or non-diluted) is added to the stripped eggs, and

the eggs fertilized by adding an equal volume of clean water. The water and egg mass

are then mixed by gently shaking the bowl. Eggs must be stirred continuously until they

are placed in the hatching tanks as the eggs become sticky and without stirring will stick

together into one clump. About 60 seconds after fertilization has taken place and the

sperm has lost its activity, the fertilized eggs are then ready for incubation in tanks.

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