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Cold Water Immersion Blunts Muscle Growth

This study examined the effect of cold water immersion (CWI) after resistance exercise on muscle ribosome biogenesis. Nine men performed resistance exercise followed by either active recovery or CWI. Biopsies before and after exercise showed that markers of ribosome biogenesis like UBF1, c-Myc, and pre-rRNA transcripts increased more after active recovery than CWI. CWI also blunted the exercise-induced increases in proteins like MNK1 and eIF4E that activate protein synthesis. Therefore, CWI appears to attenuate the molecular signaling pathways involved in muscle hypertrophy after resistance exercise.

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5 views1 page

Cold Water Immersion Blunts Muscle Growth

This study examined the effect of cold water immersion (CWI) after resistance exercise on muscle ribosome biogenesis. Nine men performed resistance exercise followed by either active recovery or CWI. Biopsies before and after exercise showed that markers of ribosome biogenesis like UBF1, c-Myc, and pre-rRNA transcripts increased more after active recovery than CWI. CWI also blunted the exercise-induced increases in proteins like MNK1 and eIF4E that activate protein synthesis. Therefore, CWI appears to attenuate the molecular signaling pathways involved in muscle hypertrophy after resistance exercise.

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api-289666693
Copyright
© All Rights Reserved
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Available Formats
Download as PDF, TXT or read online on Scribd

The effect of resistance exercise on muscle ribosome biogenesis

is blunted by cold water immersion.


Vandr C. Figueiredo 1, Llion A. Roberts 2,3, James F. Markworth 1, Jonathan M. Peake 3,4, David Cameron-Smith 1.
1 The

BACKGROUND
Cold water immersion (CWI) is a recovery strategy applied after intense exercise.
Recent evidence shows that CWI blunts muscle hypertrophy in response to
resistance exercise (RE)1.

c-Myc and UBF1, but not UBF2, protein abundances were up-regulated at latter
time points only in ACT trial.

METHODS
UBF 1

9 young trained men completed two RE sessions followed by 10min of:


q active recovery (ACT, low intensity cycling, ~35 watts) or
q lower body CWI (10C).

2 .5

ACT

*#

CW I

2 .0

#
*

1 .5
1 .0
0 .5
0 .0
P re

2h

24h

( F o ld C h a n g e t o P R E )

c -M y c

( F o ld C h a n g e t o P R E )

Liggins Institute, The University of Auckland, Auckland, New Zealand.


School of Human Movement and Nutrition Sciences, The University of Queensland, Brisbane, Australia.
Centre of Excellence for Applied Sport Science Research, Queensland Academy of Sport, Brisbane, Australia.
School of Biomedical Sciences and Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia.

2 .5

#
*

#
*

2 .0
1 .5
1 .0
0 .5
0 .0
P re

48h

2h

24h

48h

Pre-rRNA transcripts (45S rRNA) were elevated during late stages of recovery
(24-48h post-RE).

*#

24h

#
*

0 .5

0 .0
2h

24h

0 .0
P re

*#

1 .0

P re

0 .5

48h

e IF 4 E

( F o ld C h a n g e t o P R E )

1 .5

2h

1 .0

48h

2h

2 .0

24h

48h

0 .0
2h

2 .0

24h

#
*

1 .5

1 .0

0 .5

0 .0
2h

24h

*#

*#

1 .5

1 .0

0 .5

0 .0

48h

#
*

P re

( F o ld C h a n g e t o P R E )

0 .5

2 8 S - IT S p r e r R N A

1 .0

2 .0

P re

2h

P re

2h

24h

48h

2 .0

1 .5

1 .0

0 .5

0 .0

48h

24h

48h

CONCLUSIONS

Upstream effectors (MNK-1, eIF4E and cyclin D1) and transcription factors of
ribosome biogenesis (UBF and c-Myc), together with the primary rRNA transcript
are up-regulated following RE. The application of CWI markedly blunted this effect
providing a possible mechanism for diminished muscle hypertrophy

1 .5

1 .0

0 .5

0 .0
P re

2h

24h

48h

Cyclin D1 protein levels were increased acutely at 2 and also at 24 and 48h post-RE,
while no change was observed at mRNA level

Figure legends: different from PRE within the same trial (p<0.05),
*
#, different between trials within the same time-point (p<0.05).
Reference:
1. Roberts LA, Raastad T, Markworth JF, Figueiredo VC, Egner IM, Shield
A, Cameron-Smith D, Coombes JS, Peake JM. Post-exercise cold water
immersion attenuates acute anabolic signalling and long-term adaptations
in muscle to strength training (under review).

1 .5

( F o ld C h a n g e t o P R E )

1 .5

p = 0 .0 6

2 8 S rR N A

10

*#

2 .0

#
*

P re

IT S - 5 .8 S p r e r R N A

15

P re

C y c lin D 1

ACT
CWI

( F o ld C h a n g e t o P R E )

*#

( F o ld C h a n g e t o P R E )

20

e - e IF 4 E S e r 2 0 9

( F o ld C h a n g e t o P R E )

p -M N K 1 T h r1 9 7

Phosphorylation of MNK1 (Thr297) and eIF4E (Ser209), key translation activators


of Cyclin D1, were increased 2h post-exercise.

2 .0

( F o ld C h a n g e t o P R E )

RESULTS

( F o ld C h a n g e t o P R E )

Vastus lateralis biopsies were taken at baseline, 2, 24 and 48h post-RE. Protein
levels and mRNA and rRNA were measured by Western blot and RT-PCR
respectively.

4 5 S p r e r R N A ( 5 'E T S )

p = 0 .0 8

P
MNK1

CWI

P
eIF4E

Increased protein
synthesis
capacity

CWI

CDK4
Cyclin D1

P
UBF

c-Myc

45S Pre-rRNA
Mature rRNAs

CWI

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