EFFECTS OF VARIATION IN ALKALINE CONCENTRATION IN THE
LIGNOCELLULOSIC COMPOSITION OF DRIED RIPE CARABAO MANGO
PEELINGS
Airra Mhae G. Ilagan, Angeliza Piarose C. Lawas and Michael Robert G. Samar
Bachelor of Science in Chemical Engineering
College of Engineering, Architecture and Fine Arts (CEAFA)
Batangas State University
Batangas City
ABSTRACT
Many agricultural raw materials rich in fermentable carbohydrates were tested
worldwide for bioconversion from sugar to ethanol, but the cost of carbohydrate raw
materials has become a limiting factor for large scale production by the industries
employing fermentation processes. The ripen fruit biomass as raw materials for
fermentation, enzymatic hydrolysis using microbial enzymes could be a possible
solution to reduce the energy and input costs in ethanol production. Among the fruit
crops, mango is at the fifth rank of the most significant foodstuffs after rice, corn and
milk. This pulpy fruit is more prone to spoilage due to their nature and this spoilage
occurs at the time of harvesting, storage, marketing and processing resulting in wastes.
Peels are the major by-products obtained during the processing of various fruits
and these were shown to be a good source of various bioactive compounds which
possess various beneficial effects. But, significant quantities of fruit peels (30-50% for
mango) are discarded as waste by the processing industries which cause real
environmental problems. These fruit processing wastes can be used as potential
feedstock for bioethanol production and this could also be an attractive alternate for
disposal of the polluting residues.
Pretreatment of lignocellulosic materials aims to decrease crystallinity of
cellulose, increase biomass surface area, remove hemicellulose, and break the lignin
barrier. This study aimed to determine the effects of variation in dilute alkaline
concentration in the lignocellulosic composition of dried ripe Carabao mango peelings.
The ripe Carabao mango peelings were dried and pulverized. The untreated
sample was analyzed to determine its initial lignocellulosic concentration. The sample
was found out to contain 41.51% extractives, 7.79% acid soluble lignin, 32.10%
holocellulose, 25.94% alpha-cellulose, and 6.16% hemicellulose. Based on the result,
the peelings have a high concentration of extractives and lignin thus should undergo
pretreatment to increase the cellulose content and to decrease the lignin content and
the extractives content.
The remaining sample was treated using three different concentrations of NaOH
(0.6 M, 0.8 M and 1.0 M). After two days of treatment, the sample was neutralized using
distilled water and dried to reduce the moisture content. Then, it was sent for analysis to
determine if there was a significant difference in the lignocellulosic composition. The
results was found that using 0.6 M, the sample contained 12.57% extractives, 2.37%
acid-soluble lignin, 67.43% holocellulose, 38.59% alpha-cellulose and 28.84%
hemicellulose, while using 0.8 M there was 11.10% extractives