GROUP MEMBERS
25 Letran. Rachelle Ann D.
26 Matias, Charmaine Joyce M.
27 Miranda, Danielle Allyson O.
28 Modina, Stella Madj Y.
29 Ortiz, Carla Jellyn
30 Panugayan, Shawn Wilgie M.
I. Title: Necessity of Oxygen in Respiration
II. Materials: germinating mung beans
25ml o f 10% KOH
25ml pyrogallic acid
50 ml distilled water
250 ml Erlenmeyer flask (2)
Stopper (2)
Tulle bag
Carbon paper
III. Objectives:
To know the necessity of the oxygen in
respiration
To know the role of KOH and
pyrogallic acid in the set-up.
To differentiate the result between the
two flasks with different solutions.
Procedure:
IV. Results:
H
2
o Pyrogallic
acid and KOH
GERMINATION Germinated Did not
germinate
Mongo Seeds suspended in 50mL water
Mongo Seeds suspended in 50mL Pyrogallic
acid and KOH
V. Explanation:
The results showed that the mongo
seeds suspended in the flask which contained
H
2
Ocontinued to germinate and give rise to
more roots while the mongo seeds suspended in
the flask which contained pyrogallic acid
(C
6
H
6
O
3
) and Potassium hydroxide (KOH) were
not able to germinate. C
6
H
6
O
3
together with
KOH absorbs the oxygen, carbon dioxide and
water needed for respiration. Because the
chemicals absorbed everything needed for
respiration, the mongo seeds were not able to
germinate. Unlike the other flask filled with
distilled water, the seeds were able to germinate
because nothing hinders the uptake of oxygen.
VI. Conclusion
Plants need oxygen in respiration and
the mung beans in the flask containing
pyrogallic acid and KOH absorbed the oxygen
needed by the plants, therefore the mongo seeds
were not able to germinate. The flask that was
filled with water did not contain any chemical
that hinders the uptake of oxygen so the mongo
seeds germinated. Therefore, the mongo seeds
need oxygen in order to germinate. These types
of plants that need oxygen in order to germinate
are called strict or obligate aerobes.
VII. References
[Link]
11/[Link]
[Link]
p
Botany: An Introduction to Plant Biology by
James D. Mauseth
2 tbsp of mung beans soaked overnight in water
Tulle bag with 30 pieces mung beans
Suspend 1 bag in a 50 mL
distlled water is a flask.
Suspend 1 bag in a 50 mL
KOH & pyrogallic acid in a
flask.
Cover both flasks with carbon paper.
Put the flasks in a dark chamber for
two days
Record the results.