Ch4 Identification Notes
Ch4 Identification Notes
Learning Objectives
Define identification and the corpus delicti, and list the complete identification data set.
Determine race from skull, complexion, eyes, and hair characteristics.
Determine sex from external characteristics, sex chromatin, and the complete skeletal comparison (skull, mandible, pelvis, long bones).
Classify intersex conditions (Klinefelter's, Turner's, true and pseudo-hermaphroditism).
Determine age from teeth (eruption, Gustafson's method), ossification centres, and other age landmarks across the lifespan.
Describe stature estimation formulas and anthropometry (Bertillon system).
Describe fingerprint classification, poroscopy, and the medicolegal use of dactylography, footprints, lip/ear prints, and retina identification.
Describe skull-photo superimposition and facial reconstruction techniques.
Describe scars, tattoo marks, occupation marks, hair examination, and forensic odontology (including bite marks) as identification tools.
Apply Nepal's legal age thresholds for criminal responsibility, marriage, and majority.
Quick Navigation: Introduction & Corpus Delicti · Identification Data · Race · Sex (External, Chromatin, Skeletal) · Intersex · Age (Teeth, Ossification, Landmarks) · Age of
Foetus · Stature · Anthropometry · Dactylography/Fingerprints · Poroscopy · Footprints/Lip/Ear/Retina Prints · Superimposition · Scars · Tattoo Marks · Occupation Marks ·
Complexion & Classic Cases · Eyes (Poison Pupil Table) · Hair · Forensic Odontology & Bite Marks · Handwriting/Voice/Gait · Nepal Law · Rapid Revision · Exam Questions
Definitions
Identification = determination of the individuality of a person based on physical characteristics (exact fixation of personality). Partial/incomplete identification =
only some facts (race, age, sex, stature) are determined.
Corpus delicti ("body of the offence") = the facts constituting a crime, e.g., in murder, the fact that a person died from unlawful violence — includes the victim's body,
the fatal weapon/bullet, bloodstained clothing, and photographs of fatal injuries. Establishing the identity of the dead body is the main part of corpus delicti. A
conviction does not strictly require corpus delicti if there are eyewitnesses or strong corroborative evidence.
1. Race & religion 2. Sex 3. Age 4. Development & stature 5. Complexion & features
10. Personal effects 11. Handwriting 12. Speech/voice 13. Gait/habits 14. Memory/education
⭐ Exam Point
Items 11–14 are useful only in the living. Sex, age, and stature are the primary characteristics of identification — no single feature alone is reliable; a combination
is always used.
2. Race
Feature Details
Complexion Limited value — brown (Indians), fair (Europeans), black (Negroes); altered by decomposition/burning
Hair Indians: black, thin, straight/wavy. Europeans: fair/light-brown/reddish, straight/wavy. Negroes: woolly (tight spirals). Mongolian hair: coarse, circular cross-
section, dense uniform pigment. Negro hair: elongated, oval cross-section, irregular pigment. Caucasian hair: round/oval cross-section, uniform fine/coarse
pigment
Skeleton Cephalic Index is most important (see below); measured with calipers between parietal eminences (breadth) and glabella–external occipital protuberance
(length) — not tape
Cephalic Index = (Max breadth of skull ÷ Max length of skull) × 100
Race can be determined from skull measurements in 85–90% of cases. Brachycephaly = coronal suture fusion; dolicocephaly = sagittal suture fusion. The Indian skull is
Caucasian with some Negroid characters.
Caucasian Orthognathism (straight lower face); rounded skull; triangular orbits; elongated nasal aperture, sharp sill; triangular palate
Mongolian Anterior malar/midnasal projection (flatter face); square skull; rounded orbits/nasal aperture/palate; smaller limbs
Negro Facial prognathism (forward lower face); narrow elongated skull; square orbits; broad nasal aperture, guttered sill; rectangular palate; longer limbs
Religion Markers
Hindu males: uncircumcised, sacred thread, wooden bead necklace, forehead caste marks, back-of-head hair tuft, pierced ear lobes. Hindu females: vermilion on head,
silver toe rings, thali, tattoos, nose-ring in left nostril. Muslim females: nose-ring in septum only, several ear-helix piercings, usually no tattoos. Muslim males:
circumcised, knee/foot corn marks (prayer posture).
3. Sex
Sex Chromatin
Barr body (Dixon & Tarr) — planoconvex mass near the nuclear membrane in buccal smear; 0–4% of nuclei in males, 20–80% in females. Davidson body ("drumstick") —
neutrophil nuclear attachment, up to 6% of cells in females, absent in males. Quinacrine staining shows a bright Y-chromosome body in 45–80% of male cells (0–4% female);
Feulgen reaction shows fluorescent X-bodies in 50–70% of female cells (0–2% male). Sex chromatin CANNOT be assessed in decomposed bodies. Y chromosome
demonstrable in dental pulp up to 1 year postmortem.
Pubic hair Thick, rhomboidal (extends to umbilicus) Thin, triangular (mons veneris only)
Body/head hair Facial/chest hair present; head hair shorter, coarser Facial/chest hair absent; head hair longer, finer
Intersex — 4 Groups
Type Features
2a. Klinefelter's syndrome Gonadal dysgenesis; anatomically male, nuclear sex female; 47XXY; delayed puberty, gynaecomastia, azoospermia, ↓testosterone, sterility,
eunuchoidism, ↑height; hyalinised seminiferous tubules; hypergonadotropic hypogonadism
2b. Turner's syndrome Gonadal dysgenesis; anatomically female, nuclear sex male; 45XO; commonest female sex-chromosome abnormality (1:2500); webbed neck, shield
chest, wide-set nipples, short stature, primary amenorrhoea, coarctation of aorta, streak ovaries (no primordial follicles)
3. True hermaphroditism Very rare; ovary + testis, or ovotestis, with ambiguous external genitalia; gonad may be abdominal/inguinal/labioscrotal; neither gonad fully functional
4. Pseudohermaphroditism Male: XY nuclear sex but female external form (testicular feminisation, 5-α-reductase deficiency); testes in abdomen/inguinal canal. Female: XX
nuclear sex but virilised external genitalia (adrenal hyperplasia, usually 21-hydroxylase deficiency)
General Larger, massive, rougher; long bones ~8% longer; larger articular surfaces; Smaller, slender, smoother; weight 2.75 kg
weight 4.5 kg
Skull architecture Rugged, sloping forehead, prominent glabella, square lower/smaller orbits, Smooth, vertical/rounded forehead, small/absent glabella, rounded/larger orbits,
prominent mastoid, U-shaped palate, large foramen magnum small mastoid, parabolic palate, small foramen magnum
Mandible Larger/thicker, square (U-shaped) chin, gonion angle <125° (everted), Smaller/thinner, rounded chin, more obtuse gonion angle (inverted)
prominent mental tubercle
⭐ Pelvis — The Single Most Reliable Bone for Sexing (Master Table)
Acetabulum Large (52 mm), directed laterally Small (46 mm), directed anterolaterally
Obturator foramen Large, oval, base upward Small, triangular, apex forward
Pubic ramus (body of pubis) Narrow, triangular Broad, square; parturition pits posteriorly
Mnemonic — Male vs Female Pelvis: "SHiVA" for Male, "WRO" for Female
Male pelvis: Small inlet (heart) · High & narrow subpubic angle (V) · V-shaped arch · Acetabulum large-lateral. Female: Wide inlet (oval) · Rounded (U) subpubic angle ·
Obturator foramen small-triangular.
Sternum (Ashley's rule) Body >2× manubrium length; total length >149 mm Body <2× manubrium; total length <149 mm
Femur head Vertical diameter >47 mm, ⅔ sphere <45 mm, <⅔ sphere
4. Age Determination
Age is determined from: (1) teeth (2) bone ossification (3) secondary sex characters (4) general development in children. In children, teeth give better age estimates than
the skeleton.
Number 20 (4 incisors, 2 canines, 4 molars per jaw) 32 (4 incisors, 2 canines, 4 premolars, 6 molars per jaw)
Third molar full eruption indicates age >17 years. Dentition occurs earlier in the lower jaw (except lateral incisors); lower teeth erupt ~1 year before upper; wisdom tooth
erupts lower-left first. Female eruption may be ~1 year ahead of males, but female skeletal age is generally 1 year ahead of dental age.
Each criterion is scored 0–3 (0=no change, 3=maximum); error ≈ ±4–7 years (increases after 50 years). Boyde's method: counts daily cross-striation incremental lines in
enamel from the neonatal line — useful for infant age. Stack's method: uses weight/height of erupting teeth in infants.
806 ossification centres at 11 intrauterine weeks → ~450 at birth → 206 bones in the adult skeleton.
Ossification begins centrally in the epiphysis and spreads peripherally.
General rule: secondary centres that appear first are the last to fuse; late-forming epiphyses fuse sooner.
Upper limb: union occurs earlier at the elbow, later at the wrist; head of humerus is the last long-bone epiphysis to unite.
Lower limb: union occurs later at the knee, earlier at the hip and ankle.
Ageing is generally more accurate from appearance of ossification centres than from fusion/union of epiphyses.
Age Event
End of 1st year 2 carpal bones visible on X-ray (then 1 more carpal bone per year, ages 2–6, roughly)
16 years Lower end of humerus, olecranon-ulna, upper radius, metacarpals, proximal phalanges fuse — elbow joint fusion complete
17–18 years Hip joint fusion; head/trochanters of femur, acromion, lower ulna fuse
18–19 years Knee, shoulder, wrist joints fuse; inner end of clavicle centre appears
20–21 years Lower femur, upper tibia/fibula, humeral head, lower radius fuse; iliac crest, ischial tuberosity, inner clavicle end fuse (medial clavicular
epiphysis is the LAST in the body to fuse)
Age Landmark
25 years Coronal, sagittal, lambdoid sutures begin closing on the inner side
25–35 years Skull sutures start closing (outer/ectocranial); symphysis pubis changes become important
30–40 years Posterior ⅓ sagittal suture closes (outer); scapula glenoid lipping begins
40 years Xiphoid unites with sternal body; scalp hair greying begins (temples first)
40–50 years Anterior ⅓ sagittal + lower coronal close (outer); all vault sutures united both endo- and ectocranially
Arcus Senilis
Grey opaque corneal ring, separated from the limbus by clear cornea; appears from lipoid degeneration around 50+ years, complete by 60. In young adults (<40 yrs)
from hyperlipidaemia, it is called arcus juvenilis.
Doli incapax (absolute) Act by a child <7 years is never an offence (S.82)
Doli incapax (rebuttable) Child 7–12 years presumed incapable unless proven to have "sufficient maturity of understanding" (S.83)
Consent for beneficial acts Child <12 cannot validly consent to harm from a good-faith beneficial act (S.89); person >18 can consent to harm not intended to cause
death/grievous hurt (S.87)
Rape Intercourse with a girl <15 (even if wife) or any girl <18 (even with consent) is rape (S.375)
Marriage Female <18, male <21 cannot validly marry (Child Marriage Restraint Act)
Nepal Law Box — Age Thresholds (Do NOT quote IPC sections for Nepal exams)
Criminal responsibility — National Criminal Code (Muluki Aparadh Samhita), 2074 (2017), Section 13: no act by a child under 10 years constitutes
an offence (absolute incapacity — doli incapax, irrebuttable).
Children's Act, 2075 (2018): defines a "child" as anyone under 18. For children aged 10–14, there is a rebuttable presumption of incapacity (doli incapax)
— the prosecution must prove the child knew the act was seriously wrong, not merely mischievous.
Consent (National Criminal Code, Sections 15–20): consent from a person below 18 years is legally invalid; consent obtained through mistake, fear,
threat, unsoundness of mind, or undue influence is also invalid.
Marriage — Muluki Civil Code, 2074, Section 70: minimum marriage age is 20 years for both parties, with no exceptions for parental consent,
pregnancy, or custom; underage marriage is void ab initio (S.72) and criminally punishable (up to 3 years' imprisonment + NPR 30,000 fine) under National
Criminal Code S.173.
Age of sexual consent in Nepal is 18 years — sexual intercourse with a person under 18 is a criminal offence regardless of "consent."
Common exam question: "What is the minimum age for criminal responsibility and marriage under Nepali law?" — Answer: 10 years (absolute) / 10–14 years
(rebuttable presumption) for criminal responsibility under the National Criminal Code S.13; 20 years for marriage under the Muluki Civil Code S.70.
5. Stature
Variation: ~1.5–2 cm taller in morning than evening (intervertebral disc elasticity); decreases ~0.6 mm/year after age 30; 1–3 cm taller lying down; increases ~2 cm after
death (loss of muscle tone, joint/disc relaxation).
Principle: After age 21, skeletal dimensions are fixed, and body-part ratios vary considerably between individuals. Data: descriptive (hair/eye colour, nose/ear/chin shape), body
marks (moles, scars, tattoos), and body measurements (height, head diameters, arm span, finger/forearm/foot length, ear dimensions, iris colour) + front/profile photographs.
Now superseded by dactylography (fingerprinting).
⭐ Individuality of Fingerprints
Identification is by comparing ridge characteristics (endings, bifurcations, lakes, islands) and their sequence — not the overall pattern. 10–12 matching points are
accepted as proof of identity. Patterns are not inherited and cannot prove paternity; even identical twins have different fingerprints. Chance of two people sharing
identical prints ≈ 1 in 64 billion. Quetlet's rule: every natural object shows infinite variation — no two patterns are ever alike.
Poroscopy (Locard)
Study of sweat-gland pore patterns on ridges (9–18 pores/mm of ridge) — permanent, individual-specific; useful when only fragmentary prints (no full pattern) are available.
Ridgeology = study of friction ridge unit shape/size/alignment. Edgeoscopy = study of ridge edge characteristics (7 features).
Latent print development: grey powder (chalk+mercury) for light surfaces, white powder (lead carbonate/French chalk) for dark surfaces; silver nitrate for paper/wood/fabric;
iodine or osmium tetroxide vapour; laser fluorescence — even 10-year-old prints can be developed. Decomposed bodies: skin removed as a "glove," preserved in formalin;
usable dermal prints down to 0.6 mm depth even after epidermis is lost. Shrivelled fingers: 20% acetic acid soak, or paraffin/glycerin injection. Mutilation: not destroyed unless
the true dermis is destroyed (superficial burns/erosion insufficient); leprosy, electrical injury, and radiation cause permanent impairment.
Medicolegal Importance
1. Links a suspect to a crime scene/weapon
2. Identifies suicides, amnesia victims, unconscious/decomposed persons
3. Prevents newborn interchange and impersonation
4. Maintains identity records; authenticates documents
Criminals: all 10 fingers recorded. Civil purposes: left thumb impression only.
Footprints (podogram) As distinct/permanent as fingerprints; used in maternity wards to prevent infant swap; walking print > standing print; sand imprint smaller than foot,
mud/clay imprint larger; adult female step length 45–55 cm, male 63–70 cm
Lip prints (cheiloscopy) 6 characteristic patterns (vertical, branched, intersected, reticular, etc.); 24 details identified; match needs 7–9 tallying characteristics
Ear prints 4 basic shapes (oval, round, rectangular, triangular); 3 pressure levels recorded for comparison (functional, gentle, more pressure)
Retina/iris Unique from birth to death, differs even in identical twins — considered the most precise/reliable biometric; altered by cataract, glaucoma, diabetes
9. Skull-Photo Superimposition
History
First applied by Glaister & Brash in 1935 (Ruxton case). Determines whether a skull could be that of a specific missing person.
Skull and face photograph negatives are aligned using the interpupillary distance as a constant scale reference. 12 comparison points checked: eyes/canthuses, nasion,
prosthion, nasal spine, lower nasal border, upper jaw lower border, zygomas, supraorbital ridges, jaw angle, external auditory meatus, teeth.
10. Scars
Definition
Fibrous tissue covered by epithelium, without hair follicles, sweat glands, or pigment — results from healing of a dermal injury (superficial epidermis-only injuries
leave no scar). Scars are permanent.
Age of a Scar
5–6 days: firm union, reddish/bluish "angry" scar. 14 days: pale, soft, tender. 2–6 months: white, glistening, tenderness fading. >6 months: tough, no further change.
Medicolegal importance: identification; weapon-type clues; age correlates with time of assault (circumstantial evidence); linea albicantes suggest prior pregnancy; disfiguring
scars or those causing joint contracture = grievous hurt.
Multiple small puncture wounds with pigment (Indian ink/carbon-black, cinnabar-red, chromic acid-green, indigo/cobalt/Prussian-blue). Fading: superficial (epidermal-adjacent)
pigment fades over ~10 years (vermilion, ultramarine fade fastest); deep dermal pigment persists (removed only slowly by phagocytes); clothed areas retain tattoos longer than
exposed/friction areas (hands fade fastest). Faded tattoos are revealed by UV light or infrared photography; visible even in decomposed bodies after wiping off macerated
epidermis.
Erasure methods: surgical excision/grafting, thermal burn, scarification, CO₂ snow, electrolysis, caustic agents (papain-glycerine, zinc chloride, tannic acid), laser (vaporises
dye particles).
Medicolegal Importance
Positive identification (initials, dates, regimental marks); indicates religion, deity of worship, culture/lifestyle, geographic origin; indecent designs suggest perversion;
reveals travel/war/occupation history; gang or drug-user affiliation markers; homosexual identifier tattoos (historically, base of thumb-forefinger web).
Type Examples
Recent/temporary Paint (painters), grease (mechanics), flour (bakers), dye (dye-workers), sawdust (timber workers)
Permanent Palmar thickening (butchers); needle marks on left index finger (tailors); "blue scars" on face/arms from coal dust (miners); hand burn scars
(blacksmiths); index/thumb cuts (opticians); discoloured nails (chemical/photography workers); thumb-index callosities + uneven shoulders
(carpenters); flattened left thumb/index (bricklayers); hardened left fingertips (violinists)
Category Findings
Identification Artificial eye, absence of eye(s), iris colour/shape, deep-set/bulging, squint, nystagmus, cataract
Injury Black eye, orbital fracture effusion/proptosis, gouging, lacerations, foreign bodies, chemical burns, corneal ulceration/opacity, vitreous haemorrhage,
choroid/retinal rupture, traumatic cataract
Dilated (mydriasis) Datura, atropine, belladonna, cannabis, cocaine, alcohol, CO, ergot, HCN, antihistamines, cyclic antidepressants, amphetamines
Constricted (miosis) Opium/opioids, organophosphorus compounds, physostigmine, pilocarpine, nicotine, barbiturates, benzodiazepines
Trichology = study of hair. Grows at ~0.4 mm/day. 3 layers: cuticle (outer, non-pigmented scales), cortex (middle, pigment-bearing fibrils), medulla (inner, keratinised).
Cortex Thick, well-striated, 4–10× medulla width Thin, rarely >2× medulla width
Medicolegal Importance
1. Connects weapon/accused/scene to victim (crime investigation)
2. Identifies the causative weapon from tip/shaft damage pattern
3. Aids identification (dyeing, bleaching, waving)
4. Estimates age and sex
5. Singeing indicates burns or close-range firearm discharge
6. Differentiates scalds from burns
7. Detects chronic heavy-metal poisoning (arsenic) via hair analysis
Scope
Application of dentistry to justice — bite-mark analysis, injury interpretation, malpractice, and dental identification. No two persons have identical dentition (adult
teeth have 160 comparable surfaces).
Pink Teeth
Seen in decomposed/skeletonised bodies near the gum-line, from protoporphyrin deposition — cause unknown; asphyxia/CO poisoning have been disproved as
causes (a classic exam trap).
Changes over time Compatible inconsistencies (wear, dental work) Unchanged for life
Records Useless without antemortem dental records Possible even without prior records (fresh comparison)
Bite Marks
Human bites: semicircular/crescentic (front teeth — incisors/canines), gap at each side; appear as two opposing "bows." Sucking action → petechiae (may become confluent
contusions in forcible bites). Sexual (love) bites: breast, neck, cheek, abdomen, arms, thighs, genitalia — teeth marks may be absent, only suck-mark petechiae seen. Self-
inflicted bites: typically shoulders/arms.
System Method
Universal System 1–16 upper right→left, 17–32 lower left→right (used by ASFO)
Haderup System Like Palmer's, but "+" for upper teeth, "−" for lower
FDI two-digit system First digit = quadrant, second = tooth number (e.g., lower right canine = 43)
Handwriting Individual-characteristic, especially rapid writing; can be disguised/forged; altered by mental/nervous disease, rheumatism; expert evidence is opinion
evidence only, not conclusive (see Beck Case)
Speech/voice Stammering/stuttering/lisping/nasal twang more evident under excitement; alterable at will — risky for identification alone; acoustic spectrogram analysis is
objective and used to trap anonymous callers
Gait Recollection-based identification (by friends/relatives) is unreliable; alterable by accident or deliberate disguise
Corpus delicti = facts proving a crime occurred; establishing victim identity is its core.
Cephalic Index = breadth/length ×100: Dolico (70–75, Aryan/Negro) < Mesati (75–80, European/Indian) < Brachy (80–85, Mongolian).
Sex chromatin unreadable in decomposed bodies; Barr body (0–4% male, 20–80% female); cannot be used post-decomposition.
Intersex: Klinefelter (47XXY, anatomic male/nuclear female) vs Turner (45XO, anatomic female/nuclear male, commonest female chromosomal abnormality).
Pelvis is THE most reliable bone for sexing: subpubic angle (V=male, U=female), sciatic notch, obturator foramen, preauricular sulcus.
Gustafson's 6 criteria (adult age from teeth): attrition, periodontosis, secondary dentin, cementum apposition, root resorption, root transparency (most
reliable, ±4–7 yrs error).
3rd molar full eruption → age >17. Elbow fuses first (16), medial clavicle fuses LAST (20–21+) among all epiphyses.
Age estimation grows unreliable after 25 years — always give a decade-range, not a precise figure.
Stature ↑2 cm after death (muscle relaxation); ↓0.6mm/yr after age 30.
Fingerprints: loops (60-70%) > whorls (25-35%) > arches (6-7%) > composite (1-2%); identified by 10-12 matching ridge points, NOT pattern alone; not
inherited; unique even in identical twins.
Assailant fingerprints on a victim register only after death (pre-death sweat cancels prints).
Skull-photo superimposition is exclusionary (can rule OUT, cannot definitively confirm).
Scars require dermal injury (epidermis-only heals without scarring); age: angry-red (5-6d) → pale/tender (14d) → white/glistening (2-6mo) → tough (6mo+).
Pink teeth in decomposed bodies = protoporphyrin deposit; NOT diagnostic of asphyxia/CO (classic trap).
Teeth resist fire/putrefaction far better than fingerprints — key in mass-disaster identification.
Poison pupil table: dilated (atropine/CO/cocaine/HCN) vs constricted (opioids/OP compounds/pilocarpine).
Medullary index: human hair <0.3, animal hair >0.5.
Nepal: criminal responsibility absolute floor = 10 years (National Criminal Code S.13); doli incapax presumption 10–14 years; marriage age = 20 years (Muluki
Civil Code S.70); sexual consent age = 18.
Short Notes
1. Cephalic index
2. Poroscopy
3. Pink teeth
4. Klinefelter's syndrome
5. Corpus delicti
6. Arcus senilis
7. Medullary index of hair
8. Sydney Shark Case
Clinical/Medicolegal Scenario
A skeletonised body is recovered. The pelvis shows a wide sciatic notch, U-shaped subpubic angle, and a deep preauricular sulcus. The pubic symphysis shows early
breakdown of the ventral margin. Estimate the sex and approximate age, and justify your reasoning.
Expected approach: Identify the pelvic features (wide sciatic notch, U-shaped/rounded subpubic angle ~90–100°, deep/frequent preauricular sulcus) as classic female
pelvic morphology; correlate the pubic symphysis changes (breakdown of ventral margin) with the decade-based age progression described in this chapter (consistent with
the fifth–sixth decade range); note that multiple skeletal criteria (skull, long bones if available) should be cross-checked before finalising an opinion, and that the final age
must be expressed as an approximate range, not an exact figure.
References: Reddy KSN, Murty OP. The Essentials of Forensic Medicine and Toxicology, 33rd Edn, Chapter 4 "Identification" (primary source). National Criminal Code (Muluki Aparadh Samhita),
2074 (2017), Nepal. Muluki Civil Code, 2074 (2017), Nepal. Children's Act, 2075 (2018), Nepal.
Prepared for Third Year MBBS (TU, Nepal) — Forensic Medicine & Toxicology self-study notes | Chapter 4 of 38