RESEARCH PROJECT

FORMULATION AND EVALUATION OF SKIN-FRIENDLY HAND SANITIZER USING

70% ETHYL ALCOHOL, ALOE VERA, LEMON EXTRACT, AND GUAVA LEAVES
EXTRACT

A Study Presented to the Faculty of

Sibulan National High School-Balugo Extension

In Final Fulfillment of the Requirements for the

Second Semester of Senior High School

BY:
JOSH MARTIN D. SIBALA

GRADE 12-HUMSS
FEBRUARY 2026

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Introduction

Hand hygiene is one of the most effective ways to prevent the spread of

infectious diseases. While proper handwashing with soap and water is highly

recommended, it is not always possible in all situations. Because of this, hand sanitizers

are widely used as an alternative for maintaining hand cleanliness, especially when

access to water and soap is limited.

Most commercially available hand sanitizers contain high levels of alcohol and

synthetic chemicals that may cause skin dryness or irritation when used frequently. This

has led to growing interest in the development of hand sanitizers made from natural and

plant-based ingredients. Aloe vera is known for its moisturizing and soothing properties,

lemon contains natural antibacterial compounds, and guava leaves have been found to

have antimicrobial potential. The combination of these ingredients may result in a hand

sanitizer that is both effective and gentle on the skin.

Rationale

This research project aims to develop hand sanitizer using aloe vera, lemon juice,

and guava leaves extracted as natural ingredients. The study seeks to explore the

potential of these components as an alternative to commercially available hand

sanitizers that rely heavily on synthetic substances.

The findings of this research may benefit individuals by providing a safer and

more natural option for hand hygiene. Schools, communities, and health-related

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institutions may also benefit from the results by using the study as a reference for

promoting affordable and locally sourced hygiene products. Furthermore, this research

may contribute to existing knowledge on herbal-based hand sanitizers and serve as a

foundation for future related studies.

Overall, this study is significant because it supports the development of effective

and skin-friendly hand hygiene products using natural resources.

Problem Statement

Proper hand hygiene is essential in preventing the spread of harmful

microorganisms. However, many commercially available hand sanitizers rely on high

alcohol content and synthetic ingredients, which may cause skin dryness, irritation, or

discomfort when used frequently. This creates a need for alternative hand hygiene

products that are both effective and safe for regular use.

This study seeks to address the following research problems:

Can a hand sanitizer formulated using 70% Ethyl Alcohol, aloe vera, lemon juice,

and guava leaves extract serve as an effective and skin-friendly alternative to

commercially available hand sanitizers?

Specifically, this research aims to answer the following questions:

What are the observable properties (such as texture, scent, and appearance) of the

hand sanitizer made from 70% Ethyl Alcohol, aloe vera, lemon juice, and guava leaves

extract?

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1. How acceptable is the formulated hand sanitizer in terms of usability and skin feel?

2. What potential benefits can be observed from using natural ingredients in hand

sanitizer formulation?

By addressing these questions, the study intends to determine the feasibility of

producing a natural-based hand sanitizer that supports proper hand hygiene without

causing adverse effects on the skin.

III. Participants

The participants of this study are high school students who have sensitive skin

and can only use hand sanitizers formulated with natural resources, specifically aloe

vera, lemon, and guava leaves extract. These participants were selected because they

are more likely to experience skin irritation from commercially available hand sanitizers

that contain strong chemical components. By involving students with sensitive skin, the

study aims to evaluate the safety, acceptability, and effectiveness of the formulated

natural hand sanitizer in preventing skin irritation while maintaining proper hand

hygiene.

IV. Action Plan / Procedure

This study will be conducted through a systematic process to develop and

evaluate a hand sanitizer formulated with 70% Ethyl Alcohol, Aloe Vera, Lemon extract,

and Guava leaves extract. The procedure will be carried out in the following steps:

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 First, all necessary materials will be prepared. These include 70% Ethyl Alcohol,

fresh aloe vera leaves, fresh lemons, guava leaves, clean containers, measuring tools,

stirring rods, strainers, and other basic household equipment. All tools and containers

will be cleaned and sanitized before use to ensure safety and prevent contamination.

Second, the plant-based ingredients will be extracted. The aloe vera gel will be

obtained by thoroughly washing the aloe leaves, peeling off the outer skin, and

collecting the clear inner gel. The lemon extract will be prepared by washing and

squeezing fresh lemons, then filtering the juice to remove seeds and pulp. The guava

leaves extract will be prepared by washing the leaves thoroughly, boiling them in water

for several minutes, allowing the solution to cool, and filtering it to remove solid

particles.

Third, the extracted ingredients will be measured and combined with 70% Ethyl

Alcohol in appropriate proportions to create the hand sanitizer solution. The mixture will

be stirred thoroughly to ensure uniform blending and proper consistency. Once fully

mixed, the finished product will be transferred into clean, sterilized, and properly labeled

containers for storage and evaluation.

V. Data Gathering Method

The data for this study were gathered through direct observation. After

successfully formulating the hand sanitizer using 70% ethyl alcohol, aloe vera, lemon

extract, and guava leaves extract, the research proceeded with the testing phase of the

study.

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 The researcher applied the formulated hand sanitizer to the selected senior high

school students with sensitive skin during the designated testing period. Before

application, the researcher ensured that their hands were clean to avoid any external

factors that could affect the results of the observation.

During and after the application of the product, the researcher carefully observed

the participants for any visible signs of skin irritation. These included redness, itching,

swelling, rashes, excessive dryness, or any indication of discomfort. The researcher

monitored the condition of their skin immediately after use and throughout the testing

period to determine whether any delayed reactions would occur.

To ensure systematic recording, the researcher used an observation checklist to

document all visible physical responses. The researcher made sure that each

observation was recorded accurately and consistently. All gathered data were compiled

at the end of the testing period for further analysis.

VI. Data Analysis

After completing the observation period, the researcher reviewed and organized

all the recorded data. Since this study relied solely on direct observation, the researcher

analyzed the data using descriptive analysis.

The researcher examined the observation records to determine whether any

participant showed visible signs of skin irritation. The presence or absence of redness,

itching, swelling, dryness, or discomfort served as the basis for my analysis.

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Because the study focused on observable physical reactions rather than numerical

survey responses, the researcher did not use complex statistical tools. Instead, the

researcher summarized the results clearly and described the overall outcomes based

on what I directly observed during the testing period.

VII. Findings and Results

Based on direct observation throughout the testing period, none of the

participants showed visible signs of skin irritation after using the formulated hand

sanitizer. The researcher did not observe any redness, itching, swelling, rashes, or

excessive dryness on the hands of the participants.

During and after application, the participants appeared comfortable and did not

demonstrate any signs of discomfort. The skin remained in normal condition, indicating

that the formulation did not cause adverse reactions under the conditions of this study.

These findings suggest that the hand sanitizer that was formulated using 70% ethyl

alcohol, aloe vera, lemon extract, and guava leaves extract is safe for external use

among senior high school students with sensitive skin. The absence of irritation

supports the effectiveness of combining natural plant-based ingredients with alcohol to

create a hand hygiene product that is both safe and gentle on the skin.

Overall, the results indicate that the developed product may serve as a suitable and

skin-friendly alternative to commercially available hand sanitizers.