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Bioinformatics Practical Manual

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7 views1 page

Bioinformatics Practical Manual

Uploaded by

aminmehar40
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Bioinformatics Practical Manual (BSc / MSc)

List of Experiments
1. Introduction to Bioinformatics databases (NCBI, GenBank).

2. Retrieval of nucleotide sequences from NCBI.

3. Sequence similarity search using BLASTn.

4. Protein similarity search using BLASTp.

5. Translation of nucleotide sequences using ExPASy Translate.

6. Identification of Open Reading Frames (ORF Finder).

7. Multiple sequence alignment using Clustal Omega.

8. Phylogenetic tree construction using MEGA software.

9. Protein structure visualization using PyMOL.

10. Motif and domain search using PROSITE.

Example Practical: BLASTn


Aim: To compare a nucleotide sequence with sequences in biological databases using
BLASTn.

Procedure:

1. Open the NCBI BLAST website.

2. Select BLASTn.

3. Paste the query DNA sequence in FASTA format.

4. Choose nucleotide database (nr/nt).

5. Click BLAST.

6. Analyze alignment results including E-value, identity and query coverage.

Result
The BLAST program identifies similar sequences present in the database and displays
alignments and statistical scores.

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