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Laboratory Algorithm LID

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Shantadevi Sajjan
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2 views1 page

Laboratory Algorithm LID

Uploaded by

Shantadevi Sajjan
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Clinical samples from

IgM ELISA in CSF for


patient with AES
respective positive
1. IgM ELISA (Serum): JEV (CSF & Serum), DENV, pathogen(s)
CHIKV, OT, Leptospira sp
First line tests Positive
2. NS1 ELISA (Serum)*: DENV

3. Rapid Diagnostic Test (Whole Blood): Malaria


Confirmatory test for
pathogens (based on
result of first line tests)
Negative/ Clinical
suspicion
Real time PCR (CSF) JEV : Real time PCR (CSF)
1. Bacterial PCR (Streptococcus pneumoniae, Neisseria
meningitidis, Hemophilus influenzae) DENV : Real time Serotype 1-4 PCR (CSF & Blood), NS1 ELISA
Second line tests
2. Viral PCR (Herpes simplex virus 1&2, Varicella zoster CHIKV : Real time PCR (CSF & Blood)
virus, Mumps virus, Enterovirus, Parechovirus)
Negative/ Clinical OT : Real time PCR (CSF & Blood)
Suspicion

IgM ELISA in CSF for


Third line tests IgM ELISA (Serum): Measles, West Nile Virus Positive respective positive
pathogen(s)

Negative

1. Real time PCR


Additional tests on CHIKV (CSF & Blood)
stored samples with OT (CSF & Blood)
unidentifiable Mycobacterium tuberculosis (CSF)
aetiology
2. IgM ELISA (CSF): DENV, CHIKV, OT, Leptospira sp

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