ch8 Key
1. Which of the following sigma specificity factors is involved in middle gene transcription
during SPO1 phage infection of bacteria?
A. gp33
B. gp28
C. gp34
D. gp43
E. host sigma
Blooms Level꞉ 1. Remember
Section꞉ 08.01
Weaver - Chapter 08 #1
2. Which of the following explains the events in late stage T4 infection in bacteria?
A. The host sigma factor specifies transcription of genes.
B. There is a high level of transcription of host genes.
C. Host polymerase holoenzyme directs transcription.
D. The host core enzyme participates in transcription conjunction with phage-encoded
specificity factors.
E. All of the choices are correct.
Blooms Level꞉ 2. Understand
Section꞉ 08.02
Weaver - Chapter 08 #2
3. Which of the following is mostly likely to occur when sporulatiom occurs in B. subtilis?
A. complete shutdown of transcription
B. activation of vegetative genes
C. deletion of vegetative genes
D. complex sigma-switching
E. reduction of endospore formation
Blooms Level꞉ 2. Understand
Section꞉ 08.01
Weaver - Chapter 08 #3
4. An experiment is planned to look at the transcription start site for the spoIID gene during
sporulation. Which of the following techniques would yield the most information in this
experiment?
A. southern analysis
B. northern anlaysis
C. S1 mapping
D. run-on assays
E. DNAse footprinting
Blooms Level꞉ 5. Evaluate
Section꞉ 08.01
Weaver - Chapter 08 #4
5. You are designing an experiment to investigate the presence of promoter binding sites for
holoenzymes bearing B and E. in B. subtilis. A DNA template is available along with the two
holoenzyme complexes and the in vitro transcription buffers. Which of the following is a
suitable negative control for this experiment?
A. a reaction containing a DNA lacking the two promoter regions
B. a reaction containing all the components minus UTP
C. a reaction containing DNA lacking the -10 region
D. a reaction containing DNA lacking the -35 region
E. both a reaction containing a DNA lacking the two promoter regions and a reaction
containing all the components minus UTP are correct
Blooms Level꞉ 5. Evaluate
Section꞉ 08.01
Weaver - Chapter 08 #5
6. Which of the following conditions does not lead to a change in gene expression patterns in
bacterial cells?
A. sporulation
B. heat shock
C. nutrient availability
D. nitrogen deprivation
E. none of the choices are correct.
Blooms Level꞉ 2. Understand
Section꞉ 08.01
Weaver - Chapter 08 #6
7. O, P, S, and R genes are part of the lambda phage genome. A new clone of lambda was
constructed that lacked the O and P region. Which of the following would be observed if this
new clone were used to infect bacteria?
A. The clone would not enter the cell.
B. The clone would be destroyed by the bacterial host.
C. Replication of this would be impaired in the lytic phase.
D. A rapid burst of replication would occur.
E. The clone would not enter the cell and replication of this would be impaired in the lytic
phase are correct.
Blooms Level꞉ 5. Evaluate
Section꞉ 08.03
Weaver - Chapter 08 #7
8. Predict the outcome of the removal of the N gene from the DNA sequence of the phage꞉
A. lysogenic cycle will be induced.
B. entry into the bacterial host will be blocked.
C. antitermination will be affected.
D. transcription from the left promoter will be interrupted.
E. antitermination will be affected and transcription from the left promoter will be interrupted
are correct.
Blooms Level꞉ 5. Evaluate
Section꞉ 08.03
Weaver - Chapter 08 #8
9. Which of the following is true about the early phase of T7 infection of bacteria?
A. Class II genes are the first to be transcribed.
B. The host polymerase transcribes class II genes.
C. The phage-encoded polymerase transcribes the class I phage genes.
D. Class I genes are the first to be transcribed.
E. All of the choices are correct.
Blooms Level꞉ 5. Evaluate
Section꞉ 08.02
Weaver - Chapter 08 #9
10. Which of the following proteins is not involved the lytic phase of infection of bacteria?
A. CIII
B. S10
C. NusA
D. NusG
E. NusB
Blooms Level꞉ 1. Remember
Section꞉ 08.03
Weaver - Chapter 08 #10
11. Which of the following is a possible mechanism for the action of NusA in termination?
A. It binds to the RNA polymerase causing it to stall.
B. It stimulates termination at intrinsic terminator by facilitating hairpin loop formation.
C. It binds to NusB to promote detachment of the RNA polymerase for the DNA template.
D. It interacts with S10 to promote dissociation of the RNA polymerase.
E. It binds to the RNA polymerase causing it to stall and it stimulates termination at intrinsic
terminator by facilitating hairpin loop formation.
Blooms Level꞉ 2. Understand
Section꞉ 08.03
Weaver - Chapter 08 #11
12. Which of the following is an incorrect statement regarding antitermination?
A. N is involved in termination of the later genes during l phage infection.
B. Antitermination in the l late region requires Q.
C. Five proteins collaborate in antitermination in the l immediate early gene terminators.
D. Both N is involved in termination of the later genes during l phage infection and
Antitermination in the l late region requires Q are false.
E. N is involved in termination of the later genes during l phage infection, Antitermination in
the l late region requires Q, and Five proteins collaborate in antitermination in the l immediate
early gene terminators are false.
Blooms Level꞉ 2. Understand
Section꞉ 08.03
Weaver - Chapter 08 #12
13. Which of the following is involved in maintaining lysogeny in phage?
A. PRE
B. PRM
C. PRL
D. PRO
E. all of the choices are correct.
Blooms Level꞉ 1. Remember
Section꞉ 08.03
Weaver - Chapter 08 #13
14. Which of the following statements is true about the cro gene?
A. It must be stimulated during lysogeny.
B. Its product promotes repressor activity.
C. cro repression is important to lysogeny.
D. It is adjacent to the cIII gene.
E. It must be stimulated during lysogeny and its product promotes repressor activity are
correct.
Blooms Level꞉ 1. Remember
Section꞉ 08.03
Weaver - Chapter 08 #14
15. Which of the following is true about the interaction with the repressor and RNA
polymerase at PRM?
A. Interaction between repressor and polymerase is essential for activation of transcription.
B. Interaction involves region 4 of -factor.
C. Interaction can be disrupted by the binding of CIII.
D. Interaction between repressor and polymerase is essential for activation of transcription
and interaction involves region 4 of -factor are correct.
E. Interaction between repressor and polymerase is essential for activation of transcription
and interaction can be disrupted by the binding of CIII are correct.
Blooms Level꞉ 2. Understand
Section꞉ 08.03
Weaver - Chapter 08 #15
16. Which of the following operators is a target of cI gene production?
A. OR1
B. OR2
C. OL1
D. OL2
E. all of the choices are correct.
Blooms Level꞉ 1. Remember
Section꞉ 08.03
Weaver - Chapter 08 #16
17. During an experiment to study the rate of infection of bacteria with T4, the bacterial
cultures were accidentally exposed to a UV light source. Which of the following would be
expected for this exposure?
A. recA gene is turned off.
B. Coprotease activity in RecA protein is activated.
C. SOS response is induced.
D. Coprotease activity in RecA protein is activated and SOS response is induced are correct.
E. recA gene is turned off and coprotease activity in RecA protein is activated are correct.
Blooms Level꞉ 5. Evaluate
Section꞉ 08.02
Weaver - Chapter 08 #17
18. Superinfection of lysogenized -infected E. coli is prevented because
A. the superinfecting phage DNA is rapidly degraded by the host.
B. the superinfecting phage DNA is inhibited by excess repressor binding.
C. the superinfecting phage cannot replicate due to insufficient nucleotide precursors.
D. the superinfecting phage DNA is rapidly degraded by the host and the superinfecting phage
DNA is inhibited by excess repressor binding are correct.
E. the superinfecting phage DNA is rapidly degraded by the host, the superinfecting phage DNA
is inhibited by excess repressor binding, and the superinfecting phage cannot replicate due to
insufficient nucleotide precursors are correct.
Blooms Level꞉ 2. Understand
Section꞉ 08.03
Weaver - Chapter 08 #18
19. Which of the following is a target of the Cro protein?
A. OR2
B. OR3
C. OL3
D. OR2 and OR3
E. OR3 and OL3
Blooms Level꞉ 1. Remember
Section꞉ 08.03
Weaver - Chapter 08 #19
20. ___________ ____________ bind to unfolded proteins and help them to fold properly.
Molecular chaperones
Blooms Level꞉ 1. Remember
Section꞉ 08.01
Weaver - Chapter 08 #20
21. PRE is the promoter used to establish _____________ in page.
lysogeny
Blooms Level꞉ 1. Remember
Section꞉ 08.03
Weaver - Chapter 08 #21
22. _________________ suppression occurs when a mutation in one gene suppresses the
mutation in another gene.
Intergenic
Blooms Level꞉ 1. Remember
Section꞉ 08.01
Weaver - Chapter 08 #22
23. When a lysogen suffers DNA damage, it induces the ____________ response.
SOS
Blooms Level꞉ 1. Remember
Section꞉ 08.03
Weaver - Chapter 08 #23
24. Hairpin formation is necessary for ____________________.
termination
Blooms Level꞉ 1. Remember
Section꞉ 08.03
Weaver - Chapter 08 #24
25. When phage infects a bacterium, it usually subverts the host's transcription machinery to
use as its own.
TRUE
Blooms Level꞉ 2. Understand
Section꞉ 08.01
Weaver - Chapter 08 #25
26. Transcription in eukaryotes is mediated by sigma factors.
FALSE
Blooms Level꞉ 2. Understand
Section꞉ 08.01
Weaver - Chapter 08 #26
27. Transcriptional changes that occur when organisms respond to environmental changes are
in part due to changes in RNA polymerase.
TRUE
Blooms Level꞉ 2. Understand
Section꞉ 08.01
Weaver - Chapter 08 #27
28. Heat shock genes encode proteins that lead to the rapid death of cells when they are
exposed to heat.
FALSE
Blooms Level꞉ 2. Understand
Section꞉ 08.01
Weaver - Chapter 08 #28
29. T7 phage encodes a new RNA polymerase for use in the later stages of its bacterial host.
TRUE
Blooms Level꞉ 2. Understand
Section꞉ 08.02
Weaver - Chapter 08 #29
30. Lysogeny is established in the production of sufficient repressor to bind to early operators
and prevent further early gene synthesis.
TRUE
Blooms Level꞉ 2. Understand
Section꞉ 08.03
Weaver - Chapter 08 #30
31. UV radiation can trigger the lytic cycle in phage.
TRUE
Blooms Level꞉ 2. Understand
Section꞉ 08.03
Weaver - Chapter 08 #31