Va cu ta in er s
• A Vacutainer blo od coll ecti on tube is
a sterile gla ss or plastic test tube with a
col ore d rub ber stopper creating a vacuum seal
inside of the tube, facilitating the drawing of a
pre det erm ine d volu me of liquid.
Ident ify 1 M
Ment ion conta lnt• and USES
Contains £OTA •Chela tes cakkam , i.e factor IV
hence acts as antkoa 1ulant
lthvlene l iamin e f etra lceta te
(may" - pottw lum lt3 or ltZ or Sodium Na solu}
1.a mc/EDTA/ml blood
• USEa :
• Hetn0 9ram I W■C (TID),
Platel et count ,Rae Coun t,
..... Comb
.lletlc ulocy
"••--·
tea count
• HefflO llobln ■lectropflorea,•
• HbA1 C
Citrate Tube( Blue Top )
Contains 3.8 % Trisodium
Citrate in Liquid form
• USES :
• (PT)Prothrombin time
• aPTT :Activated partial
thromboplastin time tests
• TT : Thrombin time
• 0-diamer test
• ESR estimation by Westergren
method (Black Topped Tube
available)
Fluoride (Glucose ) TUBE (GRAY TOP)
! .Identify.
[Link] containt.
3.'USe.
Contains :Sodium fluoride
and Potassium oxalate
Fluoride is an inhibitor of
glycolytic enzyme enolase.
•Use:
• blood plasma sugar
Plain tube ( )
* No Contains
• elood clots after contact
with glass and separates
serum
• USE
• All serologic tests like :
• LFT
• RFT
• Lipid Profile
• Thyroii d function tests
Identify and mention uses
It is a Wii ntrobe's tube
• Uses
• l)For ESR : red
markings from top (O)
to bottom (10)
• 2)For PCV : White
markings 10 at top and
Oat bottom.
• 3 )Preparation of Buffy
coat
[Link]
[Link] Use
[Link] Anticoagulant
It Is Westergren's Tube (Pipette)
It is used for ESR (Erythrocyte
Sedimentation Rate.-(More accurate
than Wintrobe's Method]
Normal values
Female - 0 to 10 mm at the end 1 Hr
Male - - 0 to 20 mm at the end 1 Hr
Anticoaeulant used is Trisodium
citrate 3.8 " ( ratio of 3.8 W /V )
Blue /or Black capped vial
WBC Pipette 1 Identify
[Link] Uses
.Whlta bead (Arrow) in the bulb
helps to identify the pipette ,helps
in mlxin1, wet or dry •
[Link] has markings of 0.5 ,1, 11
(Dilution is 1 in 20 as 0.5 vol.
remains In the stem , Is undiluted.)
• USES
• wee count (4000-10,000/Cmm)
• Cell counts of body fluids like: CSF,
Pleural, Asdtic ,fluid.
• Sperm count
• Platelet count
lde tlfy
USE RBC pipette for RBC
Count , High WBC count as in
Leukemias & Sperm count
• The bulb contains red
bead ( Arrow)
• Markings .5,1,101
• Dilution factor is 200 as
0.5 vol is undiluted
!.Identify
[Link] the parts
It is: Sal ah 's bone
marrow aspiration
needle
The parts are
Needle (Blue Arrow )
Stylet (Red Arrow )
Guard -Screw(Yellow arrow)
Identify
Mention Sites
• This is Jamshiedi Needle for
bone marrow biopsy
• Sites :
• 1. Posterior superior iliac
spine (PSIS)
• [Link] Superior iliac
spine (ASIC)
• [Link] process of
vertebrae
• In Infants :Upper portion of
tibia i.e tibial tuberosity
1. ld en tlf y
2. M en tlo n U se s This is deposable Lu mb ar
Pu nc tur e (L.P.) Needle.
USE :CSF Examinations
• Dia gn os tic ind ica tio ns
• Menln1ttis
• Subarachnoid haemorrh•1•
• Spinal tumours
• Th era pe uti c ind ica tio ns
• Chemotherapeutic Dru1s
• Anastasia and radio1raphic dyes
[Link] [Link] is Vim Silverman - Liver
Biopsy Needle
[Link] Uses [Link] : Canula, Stylet
(trocar)and Bifid Needle (with
fork or pron1 at the end )
• USES(lndica tions)
• Evaluation of Jaundice i.e Viral hepatitis
• Liver cirrhos;s ,Type ,Progressjon
• Storage disorders :Glycogen s.d.,
hemochromatosis, Wilson [Link]
• Evaluation of Neoplastk lesions
,Primary - Secondary
• PUO : T.8.,Sarcoidosis, leishmaniasis
Identify mention use and normal values
The instrument is Urinometer
for specific gravity of urine.
It Is calibrated at 20 degree
Celsius
([Link] required for room
temp for every 3 degree rise in
T.T. 0.001 is added & for 3
degree fall 0.001 is subtracted
from the reading.)
Normal ran1• Specific 1ravity :
Random sample •1.003 - 1.030
24 HR urine sample+ 1015 •l02S
What is value for fixed sp. p-avlty or
ISOSHENURIA 1.010
Occurs In chronic rena l failure
Ide ntif y
Me,n tion Contents [Link] astk ba1 (Sin1le) for bloo d
colle ctio n.
2. It con tain s ant i~s ula nt CPO A
Citra te ,
Sodium bi-PHOSPHTE,
Dex tros e
Aden ine
IU CU IID FL U a.;
BLOOD IMO CPDA 31ChnL ..,
Identify
Mention Use
• [Link] Is Improved
Neubars Chamber
•USES:
·• RBC Count : Ssmall
squares in the centre
• 4 large squares in the
corner(W):
• TLC, Sperm Count, AEC
• Central Square :
Platelet Count
ldenit lfy
Mention Use
• Leuckhrt's (L Molds)
metal molds
• Used For Embedding
and block making with
molten wax ,in the
tissue processing.