ARPIT GUPTA
Ph: +91-9008053963/ 7666359717 Email: [Link]@[Link] ADDRESS: 21-A, New Colony,Gumanpura, Kota (Raj) 324007
Professional Overview Over 1 year of experience as a professional Research Executive committed to maintaining cutting edge technical skills in the field of Biotechnology and to work for an organization where I can apply my acquired knowledge and work as a team to contribute to the larger success of the organization. Areas of Strength Good analytical abilities Highly motivated and a natural self starter Diligent worker and a balanced composure Good group interacting skills Integrity and commitment Accomplishment Hybridoma Techniques for Monoclonal Antibody Production: Mouse Immunization, Cell Fusion, Selection of Antibody-Secreting Hybridoma, Single-Cell Cloning for selecting Antibody-Secreting Monoclone, Enzyme Conjugation of Antibodies, Match Pair Analysis Transfection in Mammalian cells: Handling of Mammalian cells, Studying growth rate of cells, Adaptation of cells in serum free media, Gene transfection by Chemical and lipid based methods Protein Purification: Ion Exchange Chromatography, Affinity Chromatography, Gel permeation Chromatography, HPLC, Ammonium Sulfate Precipitation, Cell Culture: Hybridoma Cell Maintenance, Mass Culture of Hybridoma Cells (Invitro), Ascites Production of Monoclonal Antibodies (In-vivo), Myeloma Cell-lines Maintenance, Cryopreservation of cells Diagnostic Kit development: ELISA based diagnostic kit development and stability studies of kit against various pH, temperature and time for screening cancer markers. Immunological Assays: ELISA, Western Blot, 2-D gel electrophoresis, Immuno dot Blot, Immuno-precipitation. Protein Estimation: Lowrys Method, Bradford Method, BCA Method. Enzyme reaction studies: Studies of enzyme reaction on proteins and protein stability studies in various parameters.
Instruments Handled Analytical HPLC Column Chromatography Spectrophotometer ELISA reader and washer Electrophoresis chamber Dot Blot Western Blot and 2D Blot apparatus Inverted phase contrast Microscope And related instruments. Professional Profile Company: Biocon Ltd., Bangalore Profile: Research Intern Responsibilities: Optimization of enzyme reaction of Insulin Analogues. Crystallization of proteins Effect of different parameters on crystallization/precipitation of proteins. Forced degradation studies of end products for stability studies. Tenure : March 2012 till date Company: Yashraj Biotechnology Ltd (YBL), Navi Mumbai. YBL is a part of the YASHRAJ GROUP having diversified interests in finance, construction, agriculture, chemicals and biotechnology. Company is approved by FDA (India) licensed and ISO 13485:2003 certified. It has R & D and product development approved by the Department of Science and Industrial Research, Ministry of Science and Technology, Government of India; CPCSEA (Government of India) approve Experimental and Animal Facility GMP Documentation Cell Culture Facility as per EMEA and US-FDA Guidelines. Profile: Research Executive Responsibilities: Development of Monoclonal Antibody against Cancer Markers Development of Polyclonal Antibody against Cancer Markers Purification of Antibodies and antigen using chromatography techniques Transfection of cancer marker genes in Mammalian Cells Making project plan and doing risk assessment Documentation according to ISO 13485 guidelines Tenure: July 2010 till March 2012
Thesis / Dissertation
Production & Characterization of Murine Monoclonal Antibodies against Human Prostate Specific Antigen. Responsibilities: Immunization of antigen in mice, collection of spleen, collection of macrophages from mice, Fusion, ELISA, Western blot, Single Cell Cloning, Maintenance of hybrids, Freezing and Mass culture of clones, purification of antibodies. Tenure: July 2009- May 2010 Company: Yashraj Biotechnology Ltd. Academic Qualification Year 200610 200405 200203 Degree M. Tech (Integrated) Biotechnology Higher Secondary (XII standard) Senior Secondary (X standard) Institute Dr. D. Y. Patil Biotechnology & Bioinformatics Institute, Pune CBSE board - Emmanuel School, Kota (Science-Biology, physics, Chemistry) CBSE board - St. Pauls Senior Secondary School, Kota Outcome 70% 72% 74%
Computer Proficiency Bioinformatics Tools Proficiency for Drug Designing & Molecular Modeling C and C++ Programming certification from NIIT Microsoft Office Suite (Power Point, Excel, Access, Word etc) Extracurricular Activities Active participant in intra-college competitive and cultural activities Secured 92% in All India Mathematics Olympiad in 2003 (Xth Standard) Institutional & industrial visits to MERCK Pharma, NIO Goa, IISC Active member of college Placement Cell Personal Details DOB : 18th Dec, 1986 Sex : Male Nationality : Indian Marital Status : Single Languages Known : English, Hindi Hobbies : Networking, Travelling, Reading books. Declaration I do here by confirm that the information given in this form is true to do the best of my knowledge and belief. Arpit Gupta