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Tutorial 1 24

The document presents a tutorial on cell growth kinetics, fermentation, and chemostat operations, including calculations for maximum growth rate, yield on substrate, mass doubling time, and saturation constant based on provided data. It also covers metabolic heat generation from substrate consumption and the dynamics of batch culture growth with specific substrate allocation. Additionally, it discusses the use of ethanol in a chemostat for single-cell protein production, including oxygen transfer rates and dilution rates for optimal biomass productivity.

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MK Bhai
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4 views2 pages

Tutorial 1 24

The document presents a tutorial on cell growth kinetics, fermentation, and chemostat operations, including calculations for maximum growth rate, yield on substrate, mass doubling time, and saturation constant based on provided data. It also covers metabolic heat generation from substrate consumption and the dynamics of batch culture growth with specific substrate allocation. Additionally, it discusses the use of ethanol in a chemostat for single-cell protein production, including oxygen transfer rates and dilution rates for optimal biomass productivity.

Uploaded by

MK Bhai
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Tutorial 1; CLL-271_2024

Cell growth kinetics, fermentation and chemostat

Question 1.
A simple, batch fermentation of an aerobic bacterium growing on methanol gave the results
shown in the table. Calculate the following items given below-
a. Maximum growth rate (µmax)
b. Yield on substrate (YX/S)
c. Mass doubling time (td)
d. Saturation constant (Ks)

Time (h) X (g/L) S (g/L)

0 0.2 9.23
2 0.211 9.21
4 0.305 9.07
8 0.98 8.03
10 1.77 6.8
12 3.2 4.6
14 5.6 0.92
16 6.15 0.077
18 6.2 0

Question 2.
The maximum growth yield coefficient for Bacillus subtilis growing on methanol is 0.4 g X/g
S. The heat of combustion of cells is 21 kJ/g cells and for substrate it is 7.3 kcal/g. Determine
the metabolic heat generated by the cells per unit mass of methanol consumption.

Question 3.
Consider the growth of a microorganism in batch culture. When the substrate concentration is
high, the cell density doubles every 0.75 h, the observed substrate yield coefficient is 0.3 g
DCW/g, and substrate consumption is allocated towards biosynthesis (60%), maintenance
(10%), as well as product formation (30%). The product formation is strictly growth associated.
The batch reactor is inoculated with 0.01 g DCW/L and 10 g/L substrate.
i. Estimate the maximum cell density and the time after lag phase required to achieve it.
ii. Determine the value of the maintenance coefficient (g substrate)/g [Link]). 10% of the
substrate goes to maintaining the cells.

Question 4.
Ethanol is to be used as a substrate for single-cell protein production in a chemostat. The
available equipment can achieve an oxygen transfer rate of 10 g O2/l of liquid per hour. Assume
the kinetics of cell growth on ethanol is of the Monod type, with µm = 0.5 h–1, Ks = 30 mg/l,
YX/S = 0.5 cells/g ethanol, and YO2/S = 2 g O2/g EtOH. We wish to operate the chemostat with an
ethanol concentration in the feed of 22 g/L. We also wish to maximize the biomass productivity
and minimize the loss of unused ethanol in the effluent. Determine the required dilution rate
and whether sufficient oxygen can be provided.

Question 5.
Pseudomonas sp. has a mass doubling time of 2.4 h when grown on acetate. The saturation
constant using this substrate is 1.3 g/l (which is unusually high), and cell yield on acetate is
0.46 g cell/g acetate. If we operate a chemostat on a feed stream containing 38 g/l acetate, find
the following items mentioned below-
a. Cell concentration when the dilution rate is one-half of the maximum
b. Substrate concentration when the dilution rate is 0.8 Dmax
c. Maximum dilution rate
d. Cell productivity at 0.8 Dmax

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