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Instruments Path

The document outlines various laboratory procedures and methods for analyzing blood and tissue samples, including the preparation of dilutions, the use of specific gravity measurements, and techniques for hemoglobin estimation. It also discusses the causes of abnormal specific gravity and provides details on equipment like the Sahli's hemoglobinometer and ESR testing methods. Additionally, it mentions the use of vacutainers for blood collection and the importance of proper anticoagulants in sample processing.

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n64352271
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0% found this document useful (0 votes)
6 views17 pages

Instruments Path

The document outlines various laboratory procedures and methods for analyzing blood and tissue samples, including the preparation of dilutions, the use of specific gravity measurements, and techniques for hemoglobin estimation. It also discusses the causes of abnormal specific gravity and provides details on equipment like the Sahli's hemoglobinometer and ESR testing methods. Additionally, it mentions the use of vacutainers for blood collection and the importance of proper anticoagulants in sample processing.

Uploaded by

n64352271
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

For solute

(protein/sugar) - dilute to make 70 - 80 mL and


multiply the dilutional facior.
Other methods :
distilled [Link], Bead Method, Weigh against
Causes of Higt1 specific gravity - DM,
Causes of Low pecific gravity - proteinuria
Diabetes
Low fixed specific gravity (1.010) Insipidus
(due to chronic chronic Renal Failure
glomerulonephritis)
For solute
(protein/sugar) - dilute to make 70 - 80 mL and
multiply the dilutional facior.
Other methods :
distilled [Link], Bead Method, Weigh against
Causes of Higt1 specific gravity - DM,
Causes of Low pecific gravity - proteinuria
Diabetes
Low fixed specific gravity (1.010) Insipidus
(due to chronic chronic Renal Failure
glomerulonephritis)
EosiHematoxylin
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mark 0WESTERGREN'S
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0 ESR-
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Made up of Paraffin PARAFFIN BLOCKS:
wax.
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sectioning is krnown as embeddingfirm moulds to facilitate
Procedure blocking.
Moulds are made with
wax. L-pieces and filled with molten
Tissue is transferred to the
base of mould with
forceps.
Wax is allowed to
warm
solidify to hold the tissue.
After solidification,
remove the
Sections are cut on Rolary moulds with
from the blocks.
4-5um. microtome thickness at
Test for ESR
Two methods [a] Wintrobe's [b] Westergren's
[a] WINTROBE TUBE
NB:Always ESR is estimated first followed by PCV.

dentification: glass tube closed at one end.


It measures 11 cm in length and 2.5mm in diameter.
It is calibrated as 0-10 on left side from above downwards
and from 0-10 on the right from below upwards.
Use
Measurement of ESR - Marking 0-10 from above downwards
Measurement of PCV (hematocrit) Marking 0-10 from below upwards

Method for ESR- blood is filied with the help of Pasteur pipette up to mark,
0
in Wintrobe's tube &placec vertically.
Normal value Males :010m1hour
Females: 0-20mm 1hour
Causes of Low specific gravity
Lowfixed specific gravity Diabetes Insipidus
(1.010) in chronic renal failure (due to
chronic glomerulonephritis)
SAHLI'S HEMOGLOBINOMETER
It is used for
manual Hemoglobin estimation in a
given blood sample
It consists of
tube and sahli'sComparator,
pipette or
sahli'shemoglobin
hemoglobin pipette

SAHLI'S HEMOGLOBIN TUBE


Marked in grams percentage% (2-22) and
percentage %(10-140 below
Used for mixing blood with HCLupwards)
to
manual Hemoglobin estimation by perform
Sahli'smethod
SAHLI'S PIPETTE OR HEMOGLOBIN PIPETTE
Marked at 20ul or 0.02 ml.
No bulb
Used for pipetting blood sample for
manual
Hemoglobin estimation by Sahlils method
Causes of Low specific gravity- Dlabetes
Insipidus
Low fixed specific gravity (1.010) in chronic renal failure (due to
chronic glomerulonephritis)
SAHLI'S HEMOGLOBINOMETER
It is used for manual
Hemoglobin estimation in a
given blood sample
It consists of Comparator, sahli's
tube and sahli's pipette or hemoglobin
hemoglobin pipette

SAHLI'S HEMOGLOBIN TUBE


Marked in grams percentage% (2-22) and
percentage % (10 -140 below upwards)
antam

Used for mixing blood with HCL to perform


manual Hemoglobin estimation by Sahli's method
SAHLI'S PIPETTE OR HEMOGLOBIN PIPETTE
Marked at 20ul or 0.02 ml.
No bulb
Used for pipetting blood sample for manual
Hemoglobin estimation by Sahli's method
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