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MicroscopeNotes 7

The document provides an overview of light microscopes used in labs, detailing their advantages, disadvantages, and parts. It explains how to find and focus on a specimen, calculate total magnification, and the process for drawing specimens accurately. Key components include the eyepiece, objective lenses, stage, diaphragm, and adjustment knobs.

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5 views9 pages

MicroscopeNotes 7

The document provides an overview of light microscopes used in labs, detailing their advantages, disadvantages, and parts. It explains how to find and focus on a specimen, calculate total magnification, and the process for drawing specimens accurately. Key components include the eyepiece, objective lenses, stage, diaphragm, and adjustment knobs.

Uploaded by

gajgracie
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Microscope Notes

Microscopes
• The microscopes we use in lab are light
microscopes.
• Light microscopes work by focusing visible light on
specimens and magnifies using a system of glass
lenses.
• Advantage of light microscopes– can view living
cells and cell processes
• Disadvantage– Limited powers of magnification.
Only up to 1000x larger before things become
blurry.
Early Light Microscopes
Parts of the Light Microscope
Eyepiece/
ocular lens

Nose
Arm
Objective lenses
Stage w/
Stage clips
Diaphragm Coarse adjustment

Fine adjustment
Light source

Base
1. eye piece, contains the ocular lens. Magnifies 10x.
2. nose piece, Holds the high and low power
objective lenses; can be rotated to change
magnifications.
3. Objective lens. Magnification ranges from 4x to 10x
to 40x.
4. Stage clips- hold the slide in place.
5. Stage- supports the slide being viewed.
6. Light source- projects light upwards through the
diaphragm, the specimen (on a slide), and the
lenses.
7. Base- supports the microscope.
8. Diaphragm- regulates the amount of light on the
specimen.
9. Fine adjustment knob- moves the stage slightly to
sharpen the image. Used only with a high power
lens (10x or 40x)!
10. Coarse adjustment knob- moves the stage up and
down for focusing. Used with the low power lens
(4x).
11. Arm- used to support and hold the microscope
when carried.
To find & focus on a specimen…
1. Start with stage @ lowest point and use 4x
objective lens.
2. Move stage up with coarse adjustment knob
slowly until image is as clear as possible. Do
while looking through lens.
3. Clarify image with fine adjustment knob.
4. Switch to 10x lens & re-focus as needed.
5. Repeat with 40x if needed.
To get total magnification…
• Multiply the power of the objective lens (either 4x,
10x or 40x) by 10x– the power of the ocular lens (in
the eyepiece).
To draw your specimen…
• ON EVERY SINGLE LAB, EVER!
1. Draw specimen as accurately and honestly as
you can.
2. Color your specimen neatly if required.
3. Label drawing: Include total magnification, name
of specimen and any specific details/features of
your specimen.

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