ALYSSA AMPER – BMLS-3D NOTE:
AUBF LAB Bacteriologic examination is done first if
requested
SPECIMEN COLLECTION, HANDLING AND
Urine less than the standard volume
EVALUATION
requirements is accepted only for the
SPECIMEN COLLECTION following cases:
o Acute Renal Failure
Urine is a biohazardous substance that o Pediatric Patients
requires observance of Universal Precaution. o Geriatric Patients
Gloves should be worn at all times.
Specimen containers souhlve be clean, dry, DECREASE ANALYTES
leak – proof, with a wide mouth and flat
1. Clarity – bacterial growth and precipitation
bottom.
of amorphous material
Recommended capacity of specimen
2. Glucose – Glycolysis and bacterial use
containers is 50 mL.
3. Ketones – volatilization and bacterial
ALL SPECIMEN MUST BE LABELED
metabolism
PROPERLY
4. Bilirubin – phot-oxidation
o Patient’s Name
5. Urobilinogen – oxidation or urobilin
o Identification Number
Liver
o Date and Time of Collection
6. RBCs & WBCs – disintegration to dilute
o Additional information such as
alkaline urine
patient’s age and physician’s name
Decrease and damage RBC and
REQUISITION FORMS MUST
WBC
ACCOMPANY SPECIMENS delivered to the
7. Trichomonas – loss of motility, death
laboratory and the time the specimen id
received should be recorded on the form. TYPES OF URINE SPECIMEN
SPECIMEN IS ACCEPTED IF: 1. RANDOM SPECIMEN
Most commonly received specimen
Proper labeling
Ease of collection and convenience for
Sufficient quantity
patients
Proper container
Collected at any time
No contamination
Used for ROUTINE SCREENING
Proper preservation
TESTS
Storage condition
2. FIRST MORNING
Proper transport of specimen
Also called as 8-hour specimen
SPECIMEN IS REJECTED IF: Collected immediately on arising,
immediately delivered
Specimens is unlabeled containers Concentrated and acidic
Nonmatching labels and requisition form Prevents false-negative pregnancy
Specimens contaiminated exteriors test result
Insufficient transport For evaluating orthostatic proteinuria
For nitrate testing
3. FASTING SPECIMEN 10. MIDSTREAM-CLEAN CATCH SPECIMEN
Second voided specimen after period of Alternative to catheterized specimen
fasting Safer, less traumatic method of
Does not contain any metabolites obtaining urine for bacterial culture
Recommended for Glucose Monitoring Provide patients with: appropriate
4. 2-HOUR POST PRANDIAL cleansing material, sterile container,
Collected shortly after consuming a instructions for cleansing and voiding
routine meal and collecting again 2 11. SUPRAPUBIC ASPIRATION
hours after eating. Collected by external introduction of
For monitoring of INSULIN THERAPY needle through abdomen into the
o Test glucose or sugar bladder.
5. GLUCOSE TOLERANCE SPECIMEN For bacterial culture
Collected to correspond with the blood Also used for cytologic examination
samples drawn during an OGTT 12. POSTATITIS SPECIMEN
Tested for glucose and ketones and AKA three-glass collection
results are reported along with the blood For prostatic infection determination
test Quantitative cultures are performed on
6. 24-HOUR (TIMED) SPECIMEN ALL SPECIMENS
For analytes that exhibit diurnal variation
Patients must be instructed on the FIRST SPECIMEN: first portion of the urine, increase
procedure number of bacteria and WBC can be seen
Must begin and end the collection period
SECOND SPECIMEN: midstream portion of the urine,
with an empty bladder used as a control for bladder and kidney infection. If
All specimens should be refrigerated or positive, results from the third specimen and INVALID
kept an ice during the collection period because infected urine has contaminated the
7. EARLY AFTERNOON SPECIMEN
specimen.
For urobilinogen determination
Collected between 2pm to 4pm THIRD SPECIMEN: PROSTATE IS MASSAGED
This collection correlated with the FIRST so that prostate fluid will be passed with the
“alkaline tide” remaining urine.
8. 12-HOUR URINE
For Addis Count 13. PEDIATRIC SPECIMEN
Quantitates formed elements using Soft, clear plastic bags with
hemocytometer hypoallergenic skin adhesive to attach
9. CATHETERIZED to the genital area of both boys and
Collect under sterile conditions girls.
For bacterial culture Sterile specimens may be obtained by
Also be used to measure function of CATHETERIZATION or by
individual kidney SUPRAPUBIC ASPIRATION.
14. DRUG SPECIMEN COLLECTION
Forms of tampering of specimen include
substitution, adulteration or dilution
Urines specimen collections may be
witnessed or unwitnessed.
URINE SPECIMEN FOR DRUG ANALYSIS
VOLUME 30 to 45 mL
TEMPERATURE 32.5 to 37.7 taken within
4 minutes
CONTAINER 60 mL
Bluing dye is added to the toiler water
reservoir, to avoid adulterated specimens.