OfMMRUB

INSTRUCTIONS

BX60
SYSTEM MICROSCOPE

This instruction manual to for tho Olympus System Microscope Model BX60. To obtain optimum
performanca and to familiariie yourself fully whh the use of your microscope, we recommend
that you read this manual thoroughly before operating the microscope.
 BX60
IMPORTANT

This unit employs the UIS (universal infinit system) optical design and should be used only with UIS
eyepieces, objectives, and condensers. Less than optimum performance may result if inappropriate
accessories are used.
BX6O is a versatile microscope which can be used both with transmitted light for use in the biological
field and with reflected light for metallurgical research.
Accordingly, this instruction manual contains:

• Separate outlines of the observation procedures when the microscope is used in biological or
metallurgical research purposes.
• Explanation of the use of controls is divided into sections.
• The most important points of the various observation methods are explained.
For reflected light fluorescence microscopy also refer to the manual pertaining to the reflected
light fluorescence attachment BX-fLA.

Getting Ready

1. A microscope is a precision instrument. Handle it with care and avoid

subjecting it to sudden or severe impact

2. The 8X60 series can be used with any kind of one additional inter
mediate tube excluding U-DO dual-viewing attachment and U-MDO10
multi-viewing attachment.

3. Do not use the microscope where the following conditions exist: direct
sunlight high temperature and humidity, dust or vibrations. (Working
environment ambient temperature and hunrmfity should be in the range
of 0-40°C. 30-90%.)

4. When moving the microscope, carefully cany it with both hands by

grasping the arm as shown in the figure on the left-
* You can damage the microscope if It it lifted from the ttage.
coarse adjustment knob, or lamp housing.

5. Set the voltage selector switch on the rear of the base to the 100 -120V
or 220-240V position to match the local line voltage, using a flat-head
screwdriver. (Before shipment from the factory, the voltage selector
switch is set to 220-240V position). (See figure on the left)

6. To avoid potential shock hazard, be sure to properly ground the power

cord.

7. Always turn OFF the main switch and disconnect the power coed before
replacing the halogen bulb or fuses.
 I
Maintenance and Storage;

1 Clean lenses by wiping gently with gauze. To remove fingerprints or oil stains, wipe with gauze slightty moistened
with xylene or a mixture of ether (70%) and alcohol 130%).
* SIneo ethar and alcohol are highly flammable, be careful to keep these chemicals away from an open fire
and potential sources of electrical sparks, such as main switches.
2. Do not attempt to use organic solvents to clean the microscope components especially plastic pans To dean use
a neutral detergent
3. Do not disassemble any pan of the microscope.
4. When not using the microscope, keep it covered with the provided dust cover.

Symbols on the Microscope Frame

Caution "...

If the microscope is operated in a manner not specified by this manual, the safety of the equipment may be
impaired. In addition, the equipment may also be damaged. Always operate the equipment as outlined in this
instruction manual.
 ,• M

BX60
CONTENTS

2-1 Assembly Diagram

2-2 Detailed Assembly Procedure.

Biological Microscopy 13
Metallurgical Microscopy 15

5-1 Base 17
5-2 Brightfield/Darkfield Vertical Illuminator 20
5-3 Stage 22
5-4 Observation Tube 25
5-5 Condenser 27
5-6 Focusing Adjustment Knobs 29
5-7 Immersion Objectives (for biological purposes only) 30
5-8 Photomicrography 30

!
6-1 Transmitted Light Brightfield Observation 32
6-2 Reflected Light Brightfield/Darkfield Observation 32
6-3 Reflected Light NcmarsH Differential Interference Contrast Observation 33
6-4 Reflected Light Simple Polarized Light Observation 34
6-5 Reflected Light Fluorescence Observation 35
 NOMENCIA TVRE

1
u

Observation tuba I

Brightflold/darkfieki
collector lens unit or
universal collector
lens unit
.Eyepiece

Lamp Housing
for reflected light

^\ Microsccpo frame |

Brightfield/darkfleld mirror
cuba housing or universal
I cube housing Objective

Revolving
INosepiece

Lamp housing for

CondansarI transmitted light

Largo
stage
Inghi-r

1
 BX60

ASSEMBLY

The diagram below shows how to assem&te the various components. The numbers indicate the order of assembly.
* When assembling the microscopo, make sura that all parts are free of dust and dirt and avoid scratching
any parts or touching glass surfaces.

Ump
housing

BriohtfWdMtrkfiald
Collector tans unit
IBrightfldd/dark-
ftakf or unhmsal)

Mtchtniol nag*
with WWund
(right-hind) low driv*
co»xl»l controls ,

Largi nMchtnlctl
sum with l«fl-h«nd
(right-hand) low (Irhw
coaxial controls

* The DF (dsrkfield) insert is attached when the microscopo is used for reflected light microscopy. Be
sure to remove the Insert when the microscope is used for transmitted light microscopy.
 Attaching the Stage (U-SIC4, U-SV) ,Rg.,,

1. FuUy loosen the clamping screw © at the rear of the stage (at the
front n the case of U-SV).
2.
then tighten the clamping screw.

fig. 1

Mounting the Condenser )FIa. a

1. Turn the coarse adjustment knob © to raise the stage to its upper
limit
lAssuming there are no objectives mounted on the nosepiece yet.)
2. Tumiheco^r^heiBht
holder to its tower Emit
3. Loosen the condenser clamping screw.
4. Position the condenser with the scale markings in front and insert it
Rg.2 into the substage fork as far as it wB go.
5. Tighten the condenser clamping screw, then raise the condenser to
its upper limit

in tha substage fork.

™Ullin9 *• U'SC «•**•<"« «chrom«le condenser or the

U-UCO universal condenser, swing the top lens out of the way
before inserting the condenser.
 BX60

Mounting tKS . (Rg*. 3,*)

(Universal COBe Housing)-

\
O The following procedure does not apply to the briflhfieMWartfield mirror
cube housing.
* Loosen the screw that secures tha cube housing to the frame
with tha provided Allan screwdriver. It is located In the hole at
the right side of the arm.
1. Invert the cube housing so that the cube dovetail mounts on the turret
@ point upward.
(Dummy plates are mounted in three of the four cube positions. When
Fig. 3
you wish to use only one cube, mount it in tha empty position. When
using tw6 or more cubes, loosen the clamping screw <D and remove
the dummy ptatels) by puffing in the direction infested by the arrow,
and then mount tha actual eubslsl in its place.)

2. Hold tha cube to be mounted with its index side facing upward and
slide it all the way onto the dovetail mount Next be sure to tighten
fio-4
the cube damping screw © immediately. (Tighten all (our cube damp
ing screws.)
3. Remove the cube's magnetic index sticker rj>* end affix it to the corre
sponding turret position. (Fig. 4)
*U» a sharp abject such as the tip of a ballpoint pan or macfiinical pared to
lift tha Cuba's mfignatic indax sockar.
* The cube indices A, B.C. D® on the dovetail mount correspond
to the turret's A, B, C, D Indices (JX Make sure to match tha
attached cube correctly with the position of the removed magnetic
index sticker on the turret (Fig. 5)

Mounting the Cub« Housing. (Ro,«

1. Align the cube housing dovetail © with the dovetai)® at the front of
the arm and fully slide it into tha arm.
2. insert the Allen screwdriver through the hole ® on the right side of
the arm. then securely tighten the cube housing damping screw.

Fig. 8
 Mounting the Collector Lens Unit (Fig. 6)

1. Gently insert the collector lens unit as far as it will go into the opening
located at the rear of the arm.
2. Insert the provided screws in the four screw holes ana tighten securery
with the Allen screwdriver

Fig. 6

Attaching the Field Iris Diaphragm

(Rg.7»
and Aperture Iris Diaphragm Knobs

Rg.7
1. Insert the provided field iris diaphragm and aperture iris diaphragm
knobs through (he holes 0 on the right side of the aim. then screw
in the knobs until tight
O When using the LMJflBL universal vertical illuminator lens unit the
shutter knob should also be attached in the same manner.
2. Insert the provided field iris diaphragm and aperture iris diaphragm
centering knobs through the holes (D on both sides of the arm. then
screw in the knobs until light.
3. Insert the provided pinhda knob through the hole on the left side of
the arm, then screw in the knob until tight (U-RLBL only).
 -j

BX60

Installing therHalogen Bulb (Rga. B,

(for both Transmitted/Reflected Ughts) 9.10)

O The appropriate bulb is a 12V. 100WHAL halogen bulb (PhBips 77241.

1. Futry loosen the lamp housing damping screw© on top of the lamp
housing cover with the provided AQen screwdriver.
2. Lift the lamp housing cover Q) upward to remove it (fig. 81
3. Turn the lamp socket Q) 90* in the direction indicated by the arrow.
4. Holdtng the bulb® with gloves or a piece of gauze, depress the bulb
damping levers ® and insert the bulb pins ® MV into the pin holes
Fig. B ffl. Gently release the bub damping levers ® to their original positions
to secure the bulb. (figs. 9. 10)

* Do not touch the bulb with bare hands. If fingerprints are

accidentally left on the bulb, wipe the bulb with toft doth.

S. Slide the lamp housing cover onto the housing base from above.
fig. 9 Tighten the damping screw ® whle pressing downward on the cover.
(Fig. 3)
* Whenever you replace the bulb, first tum OFF the main switch
and wait for ths bulb and lamp socket to cool down.

fig. 10
 Attaching the Lamp Housing (Figs. 11.
to the Microscope 12.13)

| Transmitted Light Lamp Homing |

1. Using the Allen screwdriver, fully loosen the lamp housing clamping
screw © on the microscope.

2. Insert the lamp housing eoUactor unit mto the microscope unU it touches
tha lamp housing bracket on the back of tha base (lower part), then
tighten the clamping screw © (Fig. 11)
fig, 11

| Reflected Light Lamp Homing |

1. Using the Allen screwdriver, fully loosen the lamp housing damping
screw © on the reflected Tight port on the microscope. (Fig. 12)

2. Insert the lamp housing collector unit into the lamp port unfl it touches
the lamp housing bracket on the back of the base (upper pan), than
tighten the clamping screw ®. (Fig. 13)

Rg. 12

3. Insert the cord plug from the transmitted light lamp housing securely
into tha power outlet ©. Insert the cord plug from tha reflected light
lamp housing securely into the power outlet ©. IFig. 13)

Fig. 13

Mounting the Objectives (Fig. 14)

C For reflected tght observation, make sure to mount metallurgical UIS

objectives.
Mount the objectives on the revolving nosepiece in such a manner
lhat the magnification increases from tow to high power in a clockwise
direction. (Fig. 14)

Fig. 14
 BX60

Mounting tfie Revolving Noseplece* * (Rg.15)

* Whan used for metallurgical obaarvatien:

Mount tha DF insert ©. The insert will be held in place by the
magnet on tha underside of the arm.
1. Turn tha coarse adjustment knob to lower the stage all the way.
2. Using the Allen screwdriver, loosen the nosaptece clamping screw @
on the microscope.
3. Carefully slide the nosepisce along the dovatail, in the direction of the
arrow, all the way in
4. Clamp the nosepiece by tightening the nosepiace clamping screw.

Mounting the Observation Tuber * r " {Rg. is>

1. Using ihe ASen screwdriver, loosen the observation tube damping screw
©■
2. Insert the circular dovetail mount at the bottom of the observation tube
into the opening on the microscope frame, placing the observation
tube to point the binocular eyepieces towards the front Fasten the
observation tube by tightening the clamping screw.

Mounting the Eyepieces;-/ (Fig* 17,18)

go. (Fig. 17)

Whan Using the Trinocular Observation Tube (U-TR30) or

the Supar-widaflold Trlnocular Observation Tuba IU-SWTR)

When using a finder eyepiece or an eyepiece with irtci xijustn

insert this eyepiece into the right-hand eyepiece sleeve. When doing
so. make sure that the eyepiece positioning pin <j) fits into the notch
® at the bottom of tha eyepiece sleeve. (Fig. 18)

8
 I
Connecting the Power Cord (R9.
1. Make sure that the main switch (?) a on OFF.
2. Before shipment from the factory, the voltage selector switch fj) u set
to the 200-240V position. In C33e your local line vortaga is 10O-120V,
move the switch to tha 100-120V position using a flat-head screwdriver.
tnu
3. Insen the power cord into the AC receptacle ® on the frame.
4. Connect tha power cord's ground wire to the ground terminal on the
wall outlet then plug the power cord into the wall outlet, (in case of
Fig. 19 a three-prong power cord and wall outlet separate grounding is not
necessary.)
Dm
ad.
Fuse Replacement (Fig*. 20, 21)
O Before replacing fuses, set tha main switch to OFF and unplug the
Cut
power cord, (the power cord should be unptugged from the AC recepocle
to allow removal of the fuse holder.)
1. Remove the fuse holder (t) by squeezing it at both sides and puling
outward. fFtg. 20)
2. Replace both fuse3 © with new ones. {Fig. 21) Spt
* Use only specified fuses.
COr
Rg. 20 ■ni
Applicable fuse: 260V, SA slow-blow (Tims Lag)
High-Breaking-Capacity, 2 fuses
(UTTLEFUSE 215005)
Fie
Rg.21
 BX60
CONTROLS

Antlyisr insertion slot

Iwhtouh
safacur knob
Potariwr tfldor
insertion slot
Raid trie
dtaphreofn knob

tnterpupitlery distance Aperture int

adjustment tctlt ■uaphnom knob

Diopter
adjustment ring Aperture m» duphraem
ccntsrino knob

Fwld ifis
csnttring knob

Tnn«mltudA*fl«ciad
Saht (elector witeh

Mjcfoacop# mountiiig
bolt holn

filter lever (LBD) Teneion

ediuetment knob
Filter lover INWI

O The above illustration shows the microscope with the U-RIBC mirror cube housing for the brightfiQkVdarfcfiold
vertical illuminator. In the case ol the IHJRBC cube housing lor the universal vertical illuminator, the cube
selector knob is replaced by a turret.
 [I

Thaixfletononly
Be used for both
modal* U-00 and
U-MOOB.

11
 BX60

I BrightfleloVDarfcfield Vertical Illuminator Attachment (U-RLBC/U-RLBU

Collector Lens Unit

(U-RLBU

Hoto for imchflMfit of

the »p»rtur« Ut» dliprtrtflro fcnob

Hoi* for ttuchment of the tpanura

Hols for m#thimiu of

\lh« fitid lrl» (Utphrwm Imeb

Hole for •ttKhrmnt of trw fWd Irii

^diaphnflm etnmtng knob

| Universal Vertical Illuminator Attachment (U-RLBC/U-RLBL)

O Refer to section 6-5. Reflacted Light Fluorescence Observation, for operation details.

Collector lent unit

(U-URBU

12
 Biological Microscopy
The Jcflcwng section outfines the operations) procures v^ me microscope o used fw rwmid
(transmitted fight brightfieW observation). ^^
* UlS objectives tor biological microscopy should be used. Using the universal cube housing, rotate the cube
turret to en empty position. Using the mirror cube housing, slide tho cube selector cube to the DF position.
1. Press the transrruttedAellected light selector switch to select the transmitted
light mode. Turn ON the main switch and adjust the tight intensity with the
light intensity lever. (At this point leave the tight intensity preset button OFF)
(Page 17)
2. Disengage aU filters from the light path. (Pages 17. 181
a. Accesssory fitter cassette
b. Fitters built into the base
o 3. Turn the revolving noseptece to engage the 10X objective. Make sure the
revolving rtosepiece dicks into position.
4. Place a specimen on the stage. (Page 22)
(Using a Trinocular Observation Tubel
6. Push the observation tube's tight path selector knob to "binocular eyepiece
100%' (the pushed-in position). (Page 26)
& Looking through the nght eyepiece with your tight eye. turn the coarse adjustment
knob to bring the specimen into focus. After obtaining approximate focus, use
the fine adjustment knob to make final adjustments. (Page 25)
13
 j. ait

BX60

7. Looking through the left eyepiece with your left eye. turn ttie diopter adjustment
ring to focus on the specimen. (Page 25)

8 Adjust the werpuftflary distance of the eyepieces. IPage 25)

9. Adjust condenser centering and focusing. (Page 27)

10. Engage the objective to be used and readjust the tight intensity to the desired
level for observation, then readjust the focus.

11. Engage your choice of fitters into the fight path. (Pages 17, 18, 19)
a. Accessory filter cassette
b. Filters built into the base

12. Adjust the field iris diaphragm. (Page 27)

13. Adiusi the aperture ens diaphragm, (Page 28)

14
 Metallurgical Microscopy

The following section outlines the operational procedures when the microscope o used to normal metallurgical rrucroscopy
(reflected light brigritfteldAJarkfield observation) with the brightfield/darkfiald vertical illuminator. In the case of the universal
vertical illuminator. the cube selector knob is replaced by a turret.

l. Select the cube according to the observation purpose. Set the cube selector
knob © to BF.

2. Disengage the filter slider © (left side of arm), analyzer i

slider 0. and NO filter from the light path. (Page 33)

I
3. Press the transmitted/reflected light selector switch to select the reflected
light mode. Turn ON the main switch and adjust the light intensity with the
light intensity lever. (At this point leave the light intensity preset button OFF.)
(Page 171

2
m

4. Place a specimen on the stage. (Page 22)

5. Turn the revolving nosepiece to engage the 10X objective. Bring the specie'
into focus.

6. Adiusi the mterpupillary distance of the eyepieces. (Page 2SI

15
 BX60

7. Looking through the right eyepcece with your light eye. turn the coarse adjustment
knob to bring the specimen into focus. After obtaining approximate focus, use
the fine adjustment knob to make final focus adjustments. (Page 25)

B. Looking through the left eyepiece with your left eye, turn the diopter adjustment
ring to focus on the specimen. (Page 25)

9. Confirm that the illumination is adequate for the observation purpose.

IPage 32)

10. Engage the required filter.

11. Engage the objective to be used for observation, then readjust the focus and
adjust the light intensity to a suitable level for observation.

12. In case of reflected light brightfteU observation, select the best field and
aperture iris diaphragm settings in accordance with the objective and specimen.
(Pages 20. 21)

16
 USING THE CONTROLS
Voltage Indicator (Fig. 22)
1 Sliding the tight intensity lever © upward increases the voltage, making
illumination brighter.
2. The numerals to the right of the voltage indicator LEOs <D indicate the
voltage.
O For photorrttcrography. tho voStege shcukJ be approximately at the tevel
indicated by the camera symbol (r£> >.
Fig. 22
Using the Transmitted/Reflected Light
Selector Switch ~ ~- ' (Rg.22)
1. Press the transmitted/reflected light selector switch ® to select trans
mitted bght illumination or reflected light illumination.
V?Z ■■ Reflected light
o ;<■£ : Transmitted light
Engaging the Light Preset Button
J
8 [fig. 23)
in
The light preset button © makes it possible to set the light intensity
to a preselected level regardless of the position of the fight intensity
o
lever.
Fig. 23
1. Push the light preset button <D to the ON position.
2. Liang a small screwdriver, turn the preset adjustment screw ® to
obtain the required light intensity. Turning the screw docfcwse increases
brightness.
3. Switch the light preset button OFF and brightness returns to the tevsl
set by the light intensity lever.
* Moving the light intensity (ever does not affect brightness while
the light preset is ON.
Using the Light Prewt Button f*
The light preset button aDows you to temporaray adjust brightrmsu a preset level for apportions such as photorn
making it unnecessary to manually adjust the brightness each time you take a photograph.
O Before shipment from the factory, the preset level is set to an intensity that is suitable for photomicrography.
0 The light preset button is also useful when using two oWerent c^ectivm altematingty, helping y^ to avrj^ rnanuany
adiusting the brightness each time you change magnification.
Use of Accessory Filters
iRgs. 24,25,2a 27,2& 29>
(for Transmitted Light)
Accessory Filter (Fig. 241
You can place one 45 mm diameter fitter m the filter holder on the
light exit at the base of the microscope. H you need to use two or
mere filters at once, use a fitter cassette.
* When using a filter cassette, you can additionally use a single
filter with thickness of toss than 3 mm ovgr the light exit glass.
Fig. 24
17
 BX60

I Utlng Built-in Fillers (Fig. 25)

Three fitters are built into tha base of the microscope. These filters
can be engaged and disengaged using the levers located on the right
side of the base.
Each of tha three Hters Q to <$ can be engaged (IN) by turning its
lever so that the • mark on the lever is aligned with the • mark on tha
base. It can be dsengaged(OUT) by turning its lever so that the o mark
is aligned with the o mark on the base.
O Each of tha three filters can be switched IN and OUT independently
of the others.

I Using the Filter Cosaetto (Figs. 26. 27. 28. 291 I

I Loading Fitters into the Fitter Housing (Fig. 27) |

O The filter housing accommodates filters wHh a diameter of 45 mm and.

a thickness of 2.7 mm or less.
0 The filter housing has two filter levers en tha right side and one on tfta
left side.
1. Move all filter levers to the OUT position except for the one belonging
to the slot into which the filter is to be inserted.
2. Slide lever 0 to the IN position. Make sure that it clicks securety into
place. (Fig. 26)
3. Holding the lever in the position shown, put the filter into tha cassette
by inserting it in the direction indicated by tha arrow.
4. insert the other (wo filters in the same manner.

1 Mounting the Filter Housing (Figs. 27. 28 I

1. Fully loosen the filter cassette clamping screw ®. (Fig. 28)

2. Holding the filter housing above the light exit glass, align the key ©
with the slot @ and snap the filter housing into place from above.
3. Rotate the filter housing to align its sides with the base. (Fig. 28)
4. Align the damping screw © with the positioning hole ® en tha light
exit then tighten the screw to fasten the filter cassette.
* Whan the fitter cassette is installed, the stage may hit it when
lowered. Therefore, exercise caution when lowering tha stags
with the fitter cassette installed.

18
 Using tha Biter Cassette (Fie. 29) I

Fig. 29

Up to three of the above filters can be inserted into the filter cassette.
Moving the levers ® on the left and right sides of tha cassette to the
IN position bnngs the corresponding filter into the light path.

19
 BX60
(Rg.30)
Light Path Selection
aid Vertical Illuminator Attaehmant
%^^—'
1U-RLBC/U-RLBL)
Select the light path by sliding the cube selector © to tha position
indicating tha desired observation method.
* Make sure that the cube selector is moved all the way to tha
stop position*.
Universal Vertical Illuminator Attachment
IU4JRBCAJ-URBU
Fig. 30 Rotate the cube turret to engage the cube for the desired observation
method.
Q Centering the Field Iris Diaphragm <R» 3D
1. Slide the cube selector knob <J) to the BF position.
2. Rotate the revolving nosepiece to engage tha 10X objective, then place
a specimen onto the stage and bring the image into approximetB focus.
3. Pull out the field iris diaphragm knob ® on the vertical illuminator to
where the diameter of the diaphragm a at its smallest
4. Turn the two field iris diaphragm centering knobs® to adjust so that
the image of the diaphragm is centered in the field of view.
5. To check centratkm. open the diaphragm by pushing in the field iris
diaphragm knob (D until the diaphragm image touches the periphery
Fig. 31 of the field of view. H the image is not centered precisely, center it
again until so.
6. Further enlarge the field iris diaphragm diameter until Ha image just
circumscribes the field of view.
Rdd litt diaphragm lm»g»
I Using the Field Iris Diaphragm |
. Reflected light Briphtfield Observation
To obtain good image contrast adjust the diameter of the illuminating
beam in accordance with the objective in use.
Using the field iris diaphragm knob ® on the vertical illuminator, adjust
the diaphragm so that the fiald of view is circumscribed by the field
ins diaphragm in order to exclude stray light
/Ey«pi«c« fi«ld at vt«w • Reflected Unht DarHiald Observation
Always keep the field iris diaphragm knob @ pushed in to leave the
diaphragm open.
dtl Cantering the Aperture Iris Diaphragm (Figs. 32,33)
1. Slide the cube selector knob © to the BF position.
2. Rotate the revolving nosepiece to engage the 10X objective, than place
a specimen on the stage and bring the image into focus.
3. Remove the eyepieces. Looking through the eyepiece sleeves, pull
out the aperture iris diaphragm knob (3) to leave the diaphragm
approximately 70% open.
4. At this point, if the diaphragm is not centered precisely, center it again
by manipulating the aperture iris diaphragm centering knobs ®.
Rg. 32
20
 I Using the Aperture Iris Diaphragm I

• Reflected light BrightfieM Observation

In general, a good image is obtained if the diaphragm is stopped down
to 7080% of tha objective's numerical aperture.
• Reflected light Oarkfield Observation
Always keep the aperture iris diaphragm knob @ pushed in to leave the
diaphragm open.
0 Depending on the specimen, an image with good contrast and little
Rare may sometimes be obtained by keeping the aperture iris diaphragm
stopped down a Unto more. Please experiment with this to see if it
works with the particular specimen.

1 Using the Pinhole Diaphragm (Ras, 32.33) I

O When using a 2S0X objective, use the pinhole diaphragm to enhance

the effect of the aperture iris diaphragm.
1. Push in the aperture iris diaphragm knob ® to open the diaphragm.
2. Pull out the pinhole knob© to bring the pinhole diaphragm into the light
path. (Fig. 33)
* Tha pinhole diaphragm is placed at the same position as the
aperture iris diaphragm, and centration may bo lost when the
aperture iris diaphragm Is adjusted.
3. Place a mirror or other highly reflective specimen on the stage. With the
eyepieces in place, rotate the fine adjustment knob in the direction where
Fig. 33 the specimen and objective move away from each ether unta the contour
of tha pmhoie becomes voible.
4. At this point if centration of the pinhole diaphragm is imprecise, use the
two aperture tris diaphragm centering knobs (3) to adjust the centration.
5. Bring the specimen into focus again.
* If the aperture iris diaphragm is stopped down when using the
pinhole diaphragm, flare may occur.
* The pinhole diaphragm is placed so that it can be centered at the
same position as the aperture iris diaphragm. However, due to
constfuction considerations, e certain play ftKTffra. This is required
for the pinhole diaphragm performance and it does not indicate
a malfunction.
* When using the pinhole diaphragm, contaminants on the eye
pieces and photo-eyepiece may become noticeable. To prevent
this, dean eyepieces periodically.

Using the Filters (Fig. 34)

Engage the filters suitable for the particular observation.

Fig. 34

21
 , -iS

BX60

Specimen Placement (figs. 36,36.37.38.39k©

Biological Specimen (Fig*. 35.38.37)

Specimen Holder lor 2 Specimen Slides (Fig 351 |

V Turn the coarse adjustment knob © to lower the stage.

2. Open the spring-toaded curved finger (f) on the specimen holder and
place tha specimen slides on the stage horn the from.
3. After placing the slides as far as they wiD go. gently release the curved
finger.

Specimen Holder for Single Slides IFips. 36.371 |

The specimen can easily be placed by sliding it into the specimen holder
from the front (Fig. 36)
* With single slide observations, the maximum slide dimensions
are 26 X 76 mm. with a thickness of 03-1.4 mm and cover glass
thickness of 0.17 mm.
When observing very large specimens, remove the specimen
holder and use the stage as a plane stage.

Using an Oil Immersion Objective I

Adsorption of immersion oil can cause the specimen to drift In such

cases, it is recommended to use the optional specimen dip (BH2-
SCB-3) for oa immersion objectives Q). (Fig. 37)

(Figs. 38.39.40)

Race a lamp of day (Ptastidnel on a metal slide ©, place the specimen

on the day. and gently press the specimen with a hand press to stick
the specimen to the metal slide. Ifig. 39)
Be sure that the surface of tha specimen to be observed is
perpendicular to the optical axis of the microscope.

22
 1. Turn the coarse adjustment knob ® to lower the stage.
2. Open the spring-loaded curved linger (D on the specimen holder and
place tha specimen slides en the stags from the front
3. After placing the specimen as far as it will go. gentty release the curved
finger. (Fig, 39)
♦ When obsarving very large specimens, remove the specimen
holder and use the stage es a plane stage.

fig 39

U-S1C4 I

After loosening the specimen holder clamping screws ©i slide tha

specimen holder apart to place the specimen. Clamp the specimen
holder by tightening the clamping screws ©. (Fig. 40)

Adjusting the TenslorTof

the X-axis and Y-axis Khbfi* >*> "i *

O The tension of the X-axis and Y-exis knobs can be individuatty adjusted.
Turning the X adjustment knob ® or the Y adjustment knob <j) counter-
dodcwise increases tension, and turning them clockwise reduces tension.
When adjusting the tension, hold the X-axis and Y-axis knobs to keep
them from turning along with the tension adjustment knobs.
O If the tension is adjusted too tight creaking sounds may be heard
Fig, 41
dunng stags travel, and the stage may return back to its original position
when stopped. (Fig. 41)

I U-SIC4 I

O This stage has no provision for tension adjustment

1. When the Y-axis lock lever <J is engaged, travel along the Y-exis is
blocked while scan in the X-axis direction is free.
2. When releasing the lock lever, make sure to return the lever to its
original position. (Fig. 42)

Fig. 42

23
 BX60

Rotating the Stags (Rg.43)

1. Slightly loosen the stage damping screw ©.

2. The stage can be rotated both to the left and to the right by turning
it with the stage clamping screw.
* A dick may be heard and fett during rotation. However, this is
duo to the construction of the stage bfsckot and does not indicate
a malfunction.
O The rotation angle changes depending on the position of the stage
knobs.

O The U-SIC stage cannot be rotated.

Stage Height Adjustment (Rgs.44,46)

O By lowering the position of the substage. the microscope will

accommodate specimens with maximuin heights of 40 mm. This is
useful when observing metallurgical specimens end other thick objects.
1. Lower the stage to its tower Emit then remove the stage from the
microscope. (See page 3)
2. Using the Allan screwdriver, loosen the substage bracket clamping
screw © and remove the substage. (Fig. 44)
3. Turn the coarse adjustment knob and raise the focusing block (J) to
where the stopper screw (D in the ami becomes visible. (Fig. 4S1
4. Using the Allen screwdriver, loosen and remove the upper stopper
screw®.
5. Reattach substage bracket and stage.
O Store the removed stopper screw ® in a safe place so that it win not
be lost il needed again.

24
 I Interpuplllary Distance Adjustment (Fig. 46)
While looking through the eyepieces, adjust the binocular vision untl
the left and right fields ol view coincide completely. The index dot •

O Note your interpupillaiY distance so that it can be quiddy duplicated.

Fig. 48

Diopter Adjustment (Figs. 47.48)

1. Looking through the right eyepiece with your right eye. rotate the
the specimen into focus.

adjustment ring © to focus on the specimen. (Fig. 47)

I Using a Finder Eyepiece I

1. Looking through the right eyepiece with your right eye. turn the knurled

'. (Figs. 47.

2. Looking through the right eyepiece, rotate the fine adjustment knob
to bring the specimen and rectides into simultaneous focus.
3. Looking through the left eyepiece with your left eye. turn the diopter
adjustment ring (J) to locus en the specimen.

Using the Eye Shades (Fig. 49)

I When Not Wearing Eyeglasses

With the eye shades in their normal extended position, observe with
your eyes close to the eye shades.

I When Wearing Eyeglasses I

Fold the eye shades down with both hands. (Fig. 49)

Fig. 49

25
 BX60

Using' Eyepiece Micrometers <Rg. so)

Eyepiece micrometers can be inserted into WH10X-H and WH10X

Use 24 mm diam. X 1.5 mm micrometer discs. Following Fig. SO.

unscrew the micrometer frame © from the eyepiece and place a
micrometer disc © into the frame. The engraving on the micrometer
disc © should face downward into the micrometer frame ®.
Screw the micrometer frame into the eyepiece as it was before.

Light Path Selection (U-TR30, U-SWTR) (Fig. 51)

SWa the light path selector knob © to select the desired light path.
O The selector knob is ordinarily at the middle position. With dark
specimens, push the knob in. If additional light is needed for television
or photomicrography, pull the knob out

lioM pith Intensity

Symbol Application
•ahcter knob ratio

Pushod « 100* forbino- ObMivstion of dtik

i

Obsarvstion of bnght
20% for binocular
MttdlO position iionSa photo*
eyop«M. 80%
. IV obMivt-
for TV/bhatogrept-y

Puttod out 10D%forTVA)hoto- , TV ob*

grapny

26
 Condenser Centering (Figa. 62,63,64)

1. Turn the condenser height adjustment knob® and raise the condenser
to its upper limit. (Fig. 52)

2. Focus on the specimen using the 10X objective.

* When using the U-SC swing-out condenser, move the top lent
Into the tight path.

3. Rotate the field iris diaphragm ring fj> in tha direction of the arrow to ot*
where the diameter of the diaphragm image is at its smallest

fig. 62 4. Turn the condenser height adjustment knob <J) to where the diaphragm
image is seen in sharp focus within the field of view.

5. Turn the two condenser centering screws ® to move the diaphragm

imago to the center of the field of view.

6. Gradually open the field iris diaphragm. The condenser is properly

centered if the diaphragm image is centered and inscribed in the field
of view.

7. During actual use. open the field diaphragm slightly until the image
circumscribes tha field of view.

Field Iris Diaphragm J

The field iris diaphragm restricts the diameter of the beam of light
entering the objective and thus excludes extraneous ight, improving
image contrast. The diameter of the field iris should be adjusted for
objective power to the extent that it just circumscribes the field of
view. (See "Compatibility of Objectives and Condensers" on the next
page I

27
 BX60

| Aperture Iris Diaphragm

53> I

illumination system. Matching tho numerical aperture ol the illumination

system with that of the objective provides better image resolution
and contrast, and also increases the depth of focus.
• Since the contrast of microscope specimens is ordinarily low. setting
the condenser aperture iris diaphragm to 70*0% o! the NA of the
objective in use is usually recommended. When necessary, adjust the
ratio by removing the eyepiece and looking into the eyepiece sleeve
while adjusting the aperture iris diaphragm ring (J) until the image
shown in Fig. S3 is seen.

O Using the Numerical Aperture Seals

the
. (fig. 54)

Example:
With the Plan 40X (NA 0.651. set the scale to 0.65 X 0.8 - 0.5.

Compatibility of Objectives and Condernari |

•I W'w usir* ir» U-SC siwnfrou a(±>omat condenw toc^^

and um Ota field ins itsphngm m tha bssa n aperture diashrscm.

•2 AllhoushslishttyinadoQuataNA rosults in a wnwrvtat daitcr feld of vi«w vwth a I00X objacUw. tlw o(OT*™
O To otaoi banar JBumrowm. usa of tha UHJLC a (ocemmendad m pnmomoography whan using tha 2X or 4X cbfiar*.

28
 Adjusting the Coarse Adjustment (Fig. gs>

O Adjust tha coarse adjustment knob tension using the tension adjustment
ring®.
The coarse adjustment knob tension is preadjusted for easy use.
However, if desired you can change the tension using the tension
adjustment ring (JX Turning the ring in the (fraction of the arrow increases
tension, and vice versa.
The tension is too low if the stage drops by itself or focus is quickly
lost after adjustment with the fine adjustment knob. In this case, turn
Fig. SS tha ring in tha direction of the arrow to increase tension.

Using the Fine Adjustment Knob Rubber Cap (Rg. 66)

O Ordinarily, the fine adjustment knob bused with the rubber cap attached.
However, if space between the knob and Die sage consols is insufficient
the cap may be removed. Tha cap makes it easier to turn the fine
adjustment knob and to obtain more accurate focus.

Pre-focusing Lever (Rg. 67) 5

O Tha pre-focusing lever ensures that tha objective does not come in c
contact with tha specimen and simplifies focusing. After focusing on
tha specimen with the coarse adjustment knob, turn this lever 0 in
tha direction of the arrow to set an upper limit on coarse adjustment
movement After changing specimens, refccuang is easry accompSshed
by rotating die coarse adjustment knob to reach tha pre-fbcused position,
then making fine adjustments with the fine adjustment knob.
O Stage movement with the fine adjustment knob is not affected by
Fig. 57 this pre-focusing lever.

29
 BX60

Use-of Immersion Objectives (Fig. 68)

1. Focus on the specimen with a low power objective.

2. Place a drcpol immersion oil (provided] onto the specimen at the area
to be observed.
3. Turn the revolving nosepiece to engage the immersion objective, then
focus using the fine adjustment knob.
* Since any bubbles In the oil will impair the image, make sure
that the oil is free of bubbles,
a. To check for bubbles, remove the eyepiece and fuffy open the field
O WwtUWUVQ

inside the observation tube. (The pupil should appear round and bright)
b. To remove bubbles, rock the nosepecesJghtiy to move the al immersion
objective beck and forth a few times.
O If the condsnser marking shows a numerical aperture INA) of 1.0 or
more, the number applies only when oil is present between the slide
glass and the top surface of the condenser. When oil is not present
the NA is about 0.9.
4. After use. remove oil from the objective front lens by wiping with
gauze sfightty moistened with an ether (7 partsVslcohol (3 pans) mixture
or with xytene.
* Using too much xylene can dissolve the lens adhesive.

O Use a Binocular observation tube (U-TR30. U-SWTRI for photomicrography.

Piwtomicroflnjphy can be performed using the PM-10. the f>[Link]«photomieroo^[Link]«idures
for operating the photomicrographic units are described in their respective instruction manuals. Procedures specific
to this microscope are described below.

H Attaching Straight/ Photo Tube (U-SPT) - (Fig. 59)

1. Using the Allen screwdriver, loosen the damping screw (T) on the
trinocular tube photo port.
2. Align the vertical index line ® with the index dot © on the straight
photo tube, then mount the straight photo tube on the trinocular tube
photo port.
3. Tighten the damping screw ©.

30
 •■si

Photo Eyepiece (Fig. 60)

Use onfy the PE photo eyepiece for photomicrogfspny. Insert the photo
eyepiece © of your choice into the straight photo tube mounted on
the trinocular observation tube.

fig. 60

Mounting the Camera Unit (Fig. 61)

Place the camera unit directly over the circular dovetail of the straight
photo tube. Make sure the index dots 0 on the straight photo tube
and the camera unit are aligned, then damp the unit.

Selecting the Observation Tube Light Path

a
See page 26 of the 'Observation Tube" section.

I fig. 61

Focus Adjustment (Ffg. 82)

1. Camera focusing is done using the binocular section of the trinocular

observation tuba.
* Whenever you remove the focusing telescope from the camera
unit be sure to install the dust cap.
2. Insert a finder eyepiece into the right eyepiece sleeve.
3. The finder eyepiece has a built-in focusing tens with four masks and
a double crosjline. and the focus is practically the same for the focusing
lens and the camera film plane. The masks indicate the areas covered,
fig. 62 and the numerals next to the masks correspond to the magnification
of the photo eyepiece. Different finder eyepieces are available for afferent
cameras. Select the type that is appropriate for the camera being used.
4. Because of the great depth of focus of 1.25X to 4X objectives, use
of the focusing magnifier (OFT) is recommended for accurate focusing.
O When using low power objectives, focusing may be accomplished
easier usmg the focusing telescope on the camera unit rather than the
finder eyepiece.

31
 BX60
OBSERVATION METHODS

* Disengage analyzer, polarizer, glare shialding ND filter, and NO fitter. No Influence on reflected light observation.

flj Selecting the Light Path for Observation (Ftg.63)

I Mirror Cuba Housing |
Slide the cube selector knob Q all the way to the DF position.

| Cube Housing |
Rotate the cube turret 10 engage the empty cube compartment.

Selecting the Light Path for Observation (Fig. mi

I Mirror Cube Homing!

Select the light path by sliding the cubo selector 0 to the position
indicating the desired observation method (BF or OF).

I Cube Housing \

Rotate the cube turret to engage either the BF or OF cube.

Glare Shielding ND Filter (U-DND) ff^j. ea

1. Insert the glare shielding NO filter 0 with the side with inscription
facing forward into the filter slot on the right side of the vertical
illuminator.
2. As you insert the filter, you will hear two dicks. At the first the filter
is in the empty position, and at the second the filter is in the light
path.
3. Ordinarily, if the NO filter is in the light path, it wdl prevent the glare

O When the illumination is too low during brightfiaW observation, or if

needed to shorten the exposure time during photomicrography, or to
brighten the field of view during darfcfield observation remove the filter
from the light path.

32
 Selecting the Light Perth for Observation (Fig. 66)

I Mirror Cube Housing"! 1. Pi,

1. Slide the cube selector knob 0 all the way to the BF position. 2. Ac

3. Si
Cube Housing J 4. Re
Rotate the cube turret to engage the BF cube in the light path. to
O When the U-MDIC differential interference contrast cube is inserted (1)
in the cube cassette, engage the U-MOIC in the light path.

Fig. 66

Installing Analyzer and Polarizer'

O When the U-MOIC differential interference contrast cube is inserted

in the cube cassette, it is unnecessary to mount and adjust the analyzer
and polarizer. Ql
1. Insect the U-AN360 analyzer with the inscription facing upward into ©
and the U-PO polarizer with the inscription facing forward into © to
engage them both into the light path.
2. Rotate the U-AN360 polarizer dial <D until complete extinction is
obtained. 1. Lc-
Fig. 67 int
O When the rial's index mark is located on the outside, away from the
microscope, this position will almost correspond to the crossed Nicols Z Dc
position. Turn the dial around this point until complete extinction is tht
obtained.

Installing the Nomarski Prism (Fig. 68)

1. Loosen the QIC damping screw 0 at the front of the revolving nose-
piece and remove the dummy slider. Then insert the U-OICR differential
interference contrast prism @ with the side with the inscription facing
e-:
upward. Tighten the clamping screw to secure the prism.
2. If a UMPton objective is used, push in the selector lever fj). If an
LMPlan objective is used, pull out the selector lever.

1. PU
Rg. 68
Sir
2. Ac

3. Sf

33
 BX60

Ottsefvaiflbn

1. Place the specimen on the stage and move the stage to bring the specimen into focus.
2 Adjust the field iris diaphragm until the diaphragm opening circumscribes the field of view.
3. Stopping down the aperture iris diaphragm somewhat may increase the contrast
4. Rotate the prism control knob 0 of the DIC prism slider to adjust the interference color of the background, and
tQ achieve maximum contrast depending on the specimen under observation, as outlined below:
(II Rotating the prism control knob of the slider win continuously change the interference color of the background
from gray to magenta (-100-600 nm).
• if the background color is black ((tatter fringe), darkfietd like observation is possible.
• if the background color is gray, a three-dimensional looking; image with maximum contrast with grey sensitivity
can be obtained.

• If the background color is magenta, even a minor optical retardation can be observed as a color change.
• Care should be taken to keep the specimen surface dean, as even a small amount of contamination on
the surface may show up dua to the exceptionally high sensitivity of the differential interference contrast
method.

121 As differential interference contrast exhibits directional sensitivity, the use of a rotatable stage is recommended.

SKTand DarWTeld 0
1. Loosen the DIC clamping screw 0 at tho front of the revolving nosepiece. and gentfy pun the U-OICR deferential
interference contrast prism <J> out Insert the dummy slider until a dick is heard. Tighten the damping screw again.
2. Disengage both the U-AN360 analyzer and the U-PO polarizer from the light path. Rotate the turret to disengage
the U-MDIC differential interference contrast cube.

0 To prepare for simple polarized light observation using the vertical illuminator, perform step fl and in Section
6-3 Reflected Light Nomarski Differential Interference Contrast Observation outlined on page 33.

V Place the specimen on the stage and then operate the coarse and fine focus knobs to bring the specimen into focus.
Simple polarized fight observation is now possible.
2. Adjust the field iris diaphragm until the diaphragm opening circumscribes the field of view.
3. Stepping down the aperture iris diaphragm may increase the contrast somewhat

34
 (110
SV
The U-URBC cube housing to be used offers the same performance as the UHJRAunivenal reflected fight fluorescence 12) Tr
vertical fflummatcf used with the BX-HA reflected light fluorescence attachment Operation is almost similar. For
delate on reflected light fluorescence observation, see tha manual pertaining to the 8X-FLA reflected light fluorescence
attachment.
Nomenclature ,..*•«■-■ --.-•: *-. v. r, , ...-..,
6 When me U-URBC cube housing and the U-URBL collector Ions unit are combined with the BX60 frame, you get
the same configuration as in the case of the U-URA.
Universal Vertical Illuminator Univarsal Vertical Illuminator
Cuba Housing (U-URBCJ Collector Lena Unit (U-URBU
Holt for machmmt of
trftpwturn Iff* ditphrtflm knob
(4) El*
Hotf for aitachmtnt 3y:
of th* iptrtura irte
diaphragm centering knob IW
Kola for anadtrmm of
'■9'
rer
lh* <i«M \ti» <L\*ptimqm knob
Koto for anadirrant
of the fl*W Iri*
(5)Fc
, Hota for mMhmwu
I of th» thunarknob
I «6>
Observation ■'■"-\_J^: . ■'■..■•v-r-^vw ■:-.--*<&:■ ■ --■ --
• •:■ 0:
For details on observation, see the manual pertaining to tha U-FLA universal reflected tight fluorescence attachment
Also refer to tha sections on simultaneous reflected Eght fluc<esiMrcearK)trEnsnW9dl^t prase contrast obserraiion,
and simultaneous reflected Gght fluorescence and transmitted tight Nomarsfci Differential interference contrast observation.
35
 BX60
SPECIFICATIONS

36
 Bloic
cms
* Ono-hsnd operation slide hclder
URm
Urtvo
(FN26
en
Plan A
I (FN26
NoCc
UMPts
(FNM
NoCc
MPt»n
IFN2E
37
 BX60
(Q) OPTICAL CHARACTERISTICS
Biological Microscopy
Magnification
Ntnw
s
Machanical luba length Numerical apanuw
*"■■'•* ""V^CovOT gtaa tMctown
38
 Magnification Nam*
Metallurgical Microscopy
Numerical aperture
Cover glut thicbwtK
-: May bo wed with or without
Mechanical tub* length a caver glue 1. Op
O: Used without • cover gltn
a) La
b) La
we
0 fit
sc
is
na
dl C
V
•Whan MPtBveO obisctiva are usod in combination vwtn me LWJLH Ioto housing Imereury burner and xenon socket) for dsrtfald
oMervtoon. flumnation at tns ceriaheiy may ba sbghUy insuHcont depending on the specimen.
39
 BX60
W TROUBLESHOOTING GUIDE
Under certain conditions, performance of tha unit may be adversely affected by factors other than defects. If problems
occur, please review the following list and take remedal action as needed. If you cannot solve the problem after
checking the entire list, please contact your local Olympus representative for assistance.
40
 T>
I:
b
I T'
Li
a c
a) T>
kr
bl V
o\
d;
Cl T>
et
(0 C
nc
aTE
nc
dc
fl T>
cc
or
ot
«. 0
aiT
dc
tr
5. S
a\ T
v
. Cl T-
41
 BX60
CD
I
o
o
42
» tr
 OLYMPUS
OLYMPUS OPTICAL CO., LTD.
43-2, Hatagaya 2-chome, Shibuya-ku, Tokyo, Japan

OLYMPUS OPTICAL CO., (EUROPA)GMBK

(Premises/Goods delivery) Wendenstrasse 14-16,20097 Hamburg, Germany
(Letters) Postfach 1&49 08,20034 Hamburg, Germany

OIYMPUS AMERICA INC.

2 Corporate Center Drive, Melville, N.Y. 11747-3157, USA

OLYMPUS OPTICAL CO. (UK.) LTD.

2-8 Honduras Street London EC1YOTX, United Kingdom