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Microbial Count Protocol

The document outlines a protocol for microbial count in sugarcane juice, including sample collection, total plate count, coliform detection, E. coli identification, and yeast and mold counting. Samples must be collected in sterile conditions and analyzed promptly. Specific incubation temperatures and methods are provided for each type of microbial analysis.

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aakriti696gupta
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3 views1 page

Microbial Count Protocol

The document outlines a protocol for microbial count in sugarcane juice, including sample collection, total plate count, coliform detection, E. coli identification, and yeast and mold counting. Samples must be collected in sterile conditions and analyzed promptly. Specific incubation temperatures and methods are provided for each type of microbial analysis.

Uploaded by

aakriti696gupta
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Microbial Count Protocol for Sugarcane Juice

1. Sample Collection
Collect sample in sterile bottles under aseptic conditions. Store at 4°C and analyze within 1 hour.

2. Total Plate Count (TPC)


Prepare serial dilutions (10^-1 to 10^-4). Take 1 mL sample into sterile petri plates. Add Plate Count
Agar, mix and incubate at 35°C for 48 hours. Count colonies and express as CFU/mL.

3. Total Coliform & Fecal Coliform (MPN)


Inoculate 1 mL sample into lactose broth with Durham tubes. Incubate at 35°C for 24-48 hrs. Gas
formation indicates positive. Confirm using BGB broth (35°C) and EC broth (44°C). Express as
MPN/mL.

4. E. coli Detection
Streak positive EC broth on EMB agar. Incubate at 35°C for 18-24 hrs. Green metallic sheen
colonies indicate E. coli.

5. Yeast and Mold Count


Prepare dilution, take 0.1 mL and spread on PDA. Incubate at 28°C for 48 hrs. Count colonies and
express as CFU/mL.

6. Ice Sample Analysis


Melt ice aseptically and perform same tests: TPC, coliform and E. coli.

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