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Practical Pointers

The document outlines various laboratory procedures and safety precautions for conducting experiments, including data representation, substance testing, and error management. It details methods for testing for starch, reducing sugars, fats, and proteins, along with necessary steps and observations. Additionally, it emphasizes the importance of controls, reliability, and accurate measurements in experimental setups.

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xinlong
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0% found this document useful (0 votes)
2 views11 pages

Practical Pointers

The document outlines various laboratory procedures and safety precautions for conducting experiments, including data representation, substance testing, and error management. It details methods for testing for starch, reducing sugars, fats, and proteins, along with necessary steps and observations. Additionally, it emphasizes the importance of controls, reliability, and accurate measurements in experimental setups.

Uploaded by

xinlong
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

PRACTICAL'll

v e v i s i on n
PO INT ERS
DRAWINGS labels Kiwi ? ?

hair
o
tz of space provided 0 Skin
,
flesh , vascular bundles ,
seed ,

0 Line

in X ( at least 2)
pencils
a Label -

length of drawing
o magnification or write statement : magnification = -

length of specimen
D V
REPRESENTATION -2
-

DATA TABLES
.

o line graph ,

0 bcrvchhrt

o histogram o average always I dp more than reading

0 pie fuckin Mar t .


o seconds
-
whole number

' '
volume lap
USE DATA numbers o -

→ trend 0
temperature -
.
0°C I . 50L

for
EXPLAIN → reason menus

SUBSTANCES N

u potassium hydroxide : absorbs cuz rapidly

✓ Sodium hydrogen carbonate : provide cuz

0 Cobalt chlorine paper : turns from blue to pink when exposed to water vapour

o sodium carbonate I Nant :


alkaline

PLANNING

i. independent

1 .
Dependent

} .
control ( at least 2)

¢ . Repeat -

11W reliability

CONTROL : act as a control to show that - - - -


-

( eg .
enzyme is needed to digest fat ,
o enzyme experiment → substrate is always in excess .

o yeast exp why mean time taken for the indicator to turn yellow should not be calculated

( Ot
Q from all of the results for each temperature ?

µi¥µ
" '

'e "
'

for both much a inner than


o le 't tune 1 experiments is an

¥g
-
-

I me vest of the results

mailmen o The time taken for test tube I includes the time taken for coz to accum -

mute and have , into indicator while me others already have CO2 .

SAFETY PRECAUTION
o wear goggles

o use test tube under

u don't place finger below knife lol

calculate CHANGE 1 PERCENTAGE

o starting temp for - might be different →


change in temp can give the rate to give an

accurate comparison

o maximum size of cell may be different → % can give us accurate comparison


change apparatus ,

way to conduct exp .

now res n Its affected


( experimental error l
← [Link] are inaccurate X
T A ! R ! p
repeat

SOURCES of ERROR IMPROVEMENTS ( 9 accuracy 11 Veria biting ,

:÷ ÷:¥ ÷ ÷ : ÷
o biological variability leg .
Chlorophyll Cohl .
) A : shorter time interval between readings

concentration at suspension does not equate to me A: mix water before taking temperature

when Nation of in w runny 11 A : use digital thermometer instead .

A : use stopwatch instead of wall


Ex : time taken for indicator to turn colourless win clock ,

Shuter ( effect Mann 11 'M 't results Repeat the experiment to find the average

ye
or anger

temperature of suspension due to heat >


Repeat experiment to obtain average

put water wall between tube and lamb


fwm liant
.

source

o ( respiration ) irregular breathing results in

the amount of CO2 being varied

results obtained every time experiment is

conducted will be different


min .

o talk of incubation time results in inaccurate → ensure incubation time for everyone is vent at con

results as
me hurting times are different

o lice bath ) temperature may not reach 00C → add salt

÷÷÷÷:÷÷÷÷:÷÷÷÷÷÷÷÷÷÷ :: ::

rate of photosynthesis & A : start counting bubbles after bubbles are

o water potential in potatoes differ from produced at a [Link] rate

→ use 1 potato for all 12 potato strips


potato to potato

→ differing results

o water in sucrose solution might have → cover Demi dish with lid during experiment .

evaporated → lower water potential

O height of foam not consistent →

⑤? → measure height point every nine .


E : heat lost human holes between u 'd and cub because . -
-

I: Scotus tu ne l bine talk to cover holes


FOOD TESTS !

IODINE TEST for STARCH

1 .
Add a few drops of iodine solution to sample .

I .
Observe cvlonv change

3 .
it iodine solution remains brown
,
starch is absent .

It iodine solution turns from brown to bine blanc


,
starch is present

BENEDICT 'S TEST for REDUCING SUGARS

I .
Ada eanai volume of benedict 's solution to sample
boiling

z . mix mixture thoroughly neat mixture in a water bath


7 n

3 .
remove from water when colour change is observed .

4 . If benedict , solution remains bine , reducing sugars are absent


Drill -

If benedict 's solution turns from blue to Ven precipitate , reducing sugars are present
n
-

|
ETHANOL EMULSION 1757 bine to :

for fats .
- green pot trace amounts of reducing sugars

, .
[Link] ma was of , am me . ye , ,wpp , unaware ammn , * reaming sugar,

brick Ved Mt amounts rt reducing sugars


large
-

thoroughly
-

z .
Shake mixture

'
3 .
Ada mixture to 2cm a- water

4 . it white emulsion is observed ,


tuts are present .

If white emulsion is not observed fats are absent


,

Blu REI TEST for PROTEINS

1 .
And ennui volume of sodium hydroxide solution to sample

z . shave mixture thoroughly


shake well after each drop N

3 .
And copper cu ) sulfate solution drop by drop . Observe colour
change .
a

4 .
If copper all sulfate solution turns violet , proteins are present

If cooper ( Il ) sulfite solution remains blue , proteins are absent


.

[Link]#uube
DECANTING : FATS OTHERS

Z .
Add ethanol Add water

[Link]#
#

a . Decant into -
IV

\ distance → light intensity


(V

(V

w
"

j
"
s #¥ .

!IIM
Yas

⑤ ? oI¥

to
④ D,
I
tonearms
.

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