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SYLLABUS

The syllabus outlines various spectroscopic methods of analysis, including UV-Visible Spectroscopy, Atomic Absorption Spectroscopy, Infrared Spectroscopy, Mass Spectrometry, Chromatography, Nuclear Magnetic Resonance Spectroscopy, Potentiometry, Voltammetry, and Flame Photometry. Each unit covers fundamental principles, instrumentation, applications, and specific techniques related to the respective methods. The document serves as a comprehensive guide for understanding analytical methods in spectroscopy and chromatography.

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5 views4 pages

SYLLABUS

The syllabus outlines various spectroscopic methods of analysis, including UV-Visible Spectroscopy, Atomic Absorption Spectroscopy, Infrared Spectroscopy, Mass Spectrometry, Chromatography, Nuclear Magnetic Resonance Spectroscopy, Potentiometry, Voltammetry, and Flame Photometry. Each unit covers fundamental principles, instrumentation, applications, and specific techniques related to the respective methods. The document serves as a comprehensive guide for understanding analytical methods in spectroscopy and chromatography.

Uploaded by

tpvp02051
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

========= SYLLABUS ============

---------------- UNIT I ----------------------

• Introduction to spectroscopic methods of analysis:


- Origin of spectra
- Interaction of radiation with matter
- Fundamental laws of spectroscopy and selection rules
- Beer-Lambert’s law
- Classification of analytical methods
- Types of instrumental methods
- Consideration of electromagnetic radiation
• UV-Visible Spectroscopy:
- Basic principle
- Molar absorptivity
- Types of electronic transitions
- Effect of conjugation and solvents
- Instrumentation for single and double beam instruments:
▪ Choice of source
▪ Monochromator
▪ Detector
- Applications of UV-Vis spectroscopy
• Atomic Absorption Spectroscopy (AAS):
- Introduction and principle
- Sample atomization
- Flame atomizer
- Types of flames
- Factors affecting atomization efficiency
- Radiation sources: HCL, EDL
- Instrumentation for AAS
- Standard addition method of analysis
- Internal standard method of analysis
- Applications of AAS

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----------- UNIT II -----------

• Infrared Spectroscopy:
- Basic principle
- Hooke’s law
- Modes of molecular vibrations
- Fingerprint and functional group region
- Structural illustration through interpretation of data
- Instrumentation:
▪ Source
▪ Monochromator
▪ Detector
- Sampling techniques
- Applications of IR spectroscopy
• Mass Spectrometry:
- Basic principle
- Fragmentation methods:
▪ EI, CI, Electrospray, ED, LD, FAB
- Fragmentation of simple organic molecules
- Base peak and molecular ion peak
- Instrumentation (brief idea)
- Applications of IR spectroscopy

--------- UNIT III ------------

• Chromatography:
- Classification
- Basic principle
- Mechanism of separation:
▪ Adsorption

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▪ Partition
▪ Ion exchange
- Development of chromatograms:
▪ Frontal
▪ Elution
▪ Displacement methods
- Qualitative and quantitative analysis Of chromatographic
methods of analysis -
▪ Paper chromatography
▪ TLC (Thin Layer Chromatography)
▪ HPLC (High-Performance Liquid Chromatography)
▪ GLC (Gas Liquid Chromatography)

------------- UNIT IV ----------------------

• Nuclear Magnetic Resonance Spectroscopy:


- Introduction and basic principle
- Instrumentation
- Nuclear shielding and deshielding
- Chemical shift and factors affecting chemical shift
- Spin-spin interaction
- Coupling constant
- Area of signals
- Interpretation of PMR spectra of:
▪ Ethyl bromide
▪ Ethanol
▪ Acetaldehyde
▪ 1,1,2-tribromoethane
▪ Ethyl acetate
▪ Toluene
▪ Acetophenone

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-------- UNIT V ---------

• Potentiometry:
- Basic principle
- Instrumentation
- Ion-selective electrodes
- Applications
• Voltammetry:
- Basic principle
- Instrumentation
- Classification:
▪ Cyclic voltammetry
▪ Linear sweep voltammetry
▪ Pulse voltammetry
- Applications
• Flame Photometry:1
- Basic principle
- Instrumentation
- Applications

===================================================

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