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Microcystis Countstar Mira FL Pro Protocol

The protocol outlines the procedure for counting Microcystis cells using the Countstar Mira FL Pro, which employs advanced imaging and AI algorithms for accuracy. It includes steps for sample preparation, instrument setup, analysis, and data interpretation, as well as optional viability staining and quality control measures. Troubleshooting tips are provided to ensure optimal results.

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Osmin Said
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3 views1 page

Microcystis Countstar Mira FL Pro Protocol

The protocol outlines the procedure for counting Microcystis cells using the Countstar Mira FL Pro, which employs advanced imaging and AI algorithms for accuracy. It includes steps for sample preparation, instrument setup, analysis, and data interpretation, as well as optional viability staining and quality control measures. Troubleshooting tips are provided to ensure optimal results.

Uploaded by

Osmin Said
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Protocol: Counting Microcystis Cells Using the Countstar Mira FL Pro

1. Principle
The Countstar Mira FL Pro uses 10x magnification, an 8.3 MP CMOS camera, and AI algorithms
for accurate microbial cell counting.

2. Materials
- Microcystis culture or environmental sample
- Countstar Mira FL Pro instrument
- 60 µm depth Countstar disposable slides
- Pipettes and sterile tips
- PBS or filtered water

3. Sample Preparation
- Mix samples gently
- Dilute to estimated 5×10^5 – 2×10^8 cells/mL range
- Filter environmental samples with 70 µm mesh

4. Instrument Setup
- Use Brightfield or Brightfield + Fluorescence modes
- Autofluorescence from chlorophyll can help detection

5. Loading Sample
- Pipette 6 µL into the counting chamber
- Insert slide into the 5■position holder

6. Running Analysis
- Select microbial mode
- Choose imaging mode
- Automated image capture and AI-based counting

7. Data Interpretation
- Concentration, size histogram, and fluorescence (optional)

8. Viability Staining (Optional)


- Compatible with SYTO■9/PI staining

9. Quality Control
- Validate using standard beads

10. Troubleshooting
- Adjust sample dilution
- Filter samples to reduce debris

End of Protocol

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