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Basic Microscope1 Compressed

The document outlines a 20-hour course on microscopy techniques, covering optical microscopy, its principles, and limitations, as well as electron microscopy and its advantages. Key topics include the nature of light, numerical aperture, resolution, and historical milestones in microscopy development. It highlights the evolution from early microscopes to advanced electron microscopy, emphasizing the importance of these tools in biological studies.

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sirshendumukhopadhyay23
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5 views27 pages

Basic Microscope1 Compressed

The document outlines a 20-hour course on microscopy techniques, covering optical microscopy, its principles, and limitations, as well as electron microscopy and its advantages. Key topics include the nature of light, numerical aperture, resolution, and historical milestones in microscopy development. It highlights the evolution from early microscopes to advanced electron microscopy, emphasizing the importance of these tools in biological studies.

Uploaded by

sirshendumukhopadhyay23
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Microscope

SEM 1
Unit 3: Microscopy Techniques (20 hours)
Optical microscopy, the nature of light—its particle and wave character. Ray
diagrams and image formation.(4 hours) Simple and compound microscopes,
Applications of optical microscopes, Numerical Aperture (NA) Resolution,
Contrast, depth of f ield and depth of focus, Angular magnif ic ation, Spherical
ab er rat io n, Chro mat ic ab er rat io n o f o p t ical syst em (d ef in it io ns o nly).
Mathematical expression for limit of resolution in terms of Rayleigh criteria.
Empty magnification.(6 hours)
B as ic p r incip les o f o il immer s io n micro s co p e. Limit at io ns o f o p t ical
microscopes.(2 hours)
Elect ron microscopy---b asic working p rincip le, ad vant ag es of elect ron
m i c r o s c o p e o ve r o p t i c al m i c r o s c o p e , O p t i c al M i c r o s c o p y vs . T E M ,
Electrostatics and magnetostatics electron microscopes, Relation between the
applied voltage and wavelength of electrons.(8 hours)

Jayanti Guha
Ray
Mol. Biol. Dept.
Historical bacground

1665 : Robe r t Hooke const r uct e d com pound


microscope.
1674 : Anton van Leeuwenhoek, the father of biology,
was the first to use microscope for biological studies.
1830 : Achromatic lenses are made
1870 : Good quality microscope with oil immersion
lenses became available.

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