Study 1 – Aim of the Study
The study was about using marine microalgae as biostimulants to help baby leaf lettuce grow
better.
The researchers wanted to test if two types of microalgae could increase yield, plant growth,
and quality in a natural, eco-friendly way.
They did this experiment because:
Farmers need sustainable methods to grow more food as the world’s population
increases.
Lettuce and other “baby leaf” vegetables are popular and grown very fast, so improving
their growth is important.
Chemical fertilizers can harm the environment, but biostimulants from microalgae might
help plants grow using fewer chemicals.
There were not many studies testing how marine microalgae affect lettuce, so this
research tried to fill that gap.
In short, the aim was to see if marine microalgae could make lettuce grow faster, stronger, and
with better nutrient use.
Study 2 – Methods (How They Did the Experiment)
Plant used: Baby leaf lettuce, variety “Oxana.”
Place: Greenhouse at the University of Salerno, Italy.
Duration: 35 days (from sowing to harvest).
Groups / Treatments:
1. Control – only water
2. Commercial biostimulant (Agrialgae®)
3. Nannochloropsis gaditana extract
4. Porphyridium sp. extract
Growing conditions:
o Temperature: 25–28 °C
o Humidity: 37–50%
o Light: 16 h light / 8 h dark
Watering: Every two days (60 mL per pot)
Fertilizer: Nitrogen added twice during the growth period
Microalgae treatments: Sprayed on the leaves once a week (4 times total)
Measurements:
o Plant height, number of leaves, and fresh/dry weight
o Chlorophyll level and temperature of leaves
o Leaf colour and area
o Gene expression to check how the treatment affected nitrogen use and growth