PHOTOSYNTHESIS IN HIGHER PLANTS (COMPLETE CHAPTER) ASSIGNMENT
1. The first action spectrum of photosynthesis:
[a] Was given by T.W. Engelmann [b] Was performed using blue and red light only
[c] Was done by taking the blue green alga, Cladophora [d] Was performed by taking anaerobic bacteria
2. Read the following statements:
(i) In Engelmann’s experiment the aerobic bacteria accumulated mainly in the region of green light of the split
spectrum.
(ii) Action spectrum of photosynthesis corresponds closely to absorption spectra of chlorophyll-a showing that
chlorophyll-a is the chief photosynthetic pigment
(iii) Some organisms do not release O2 during photosynthesis
(iv) There occurs a clear division of labour w.r.t. light reaction and dark reaction within the chloroplast
(v) Purple and green sulphur bacteria can liberate O2 during photosynthesis
Which of the above statements are correct?
[a] (i), (ii) and (iii) [b] (i) and (iv) only [c] (ii), (iii) and (iv) [d] (iii), (iv) and (v)
3. Cornelius van Neil:
[a] Based on his studies of purple and green bacteria demonstrated that hydrogen from a suitable oxidisable
compound oxidises CO2 into carbohydrates
[b] Demonstrated that photosynthesis is essentially a light independent reaction
[c] Experimented only on green plants but not bacteria
[d] A Microbiologist is known for the milestone contribution to the understanding of chemistry of photosynthesis
4. Which of the following experiment proves that CO2 is essential for photosynthesis?
[a] Moll’s half leaf experiment [b] Bell jar experiment
[c] Thistle funnel experiment [d] Both b and c
5. ‘Chlorophyll-a is the main photosynthetic pigment.’ This is justified as:
[a] The wavelengths at which thee is maximum absorption by chlorophyll-a, i.e., in the blue and red-regions, also
show higher rate of photosynthesis
[b] Chlorophyll-a is the only photosynthesis pigment which can eject electron to initiate light reaction
[c] Chlorophyll-a is abundantly present [d] More than one option is correct
6. How many NADPH and ATP molecules are used in Calvin cycle for production of trioses required for synthesis of
6 sucrose molecules
[a] 144 NADPH, 216 ATP [b] 288 NADPH, 432 ATP
[c] 144 NADPH, 288 ATP [d] 72 NADPH, 108 ATP
7. Leaf pigments of any green plant are reported by:
[a] Chromatography [b] Use of KOH
[c] Use of light of specific wavelength [d] Use can not reported
8. The water splitting complex is:
[a] Associated with the PS-II [b] Located on the inner side of the thylakoid membrane
[c] May remain associated with PS-I [d] More than one option is correct
9. During photolysis of water, protons and O2 formed are initially released in the:
[a] Intermembrane space of chloroplast [b] Thylakoid lumen
[c] Stroma [d] Both (a) and (b)
10. The phenomenon of ATP synthesis with the help of sunlight in plants is called:
[a] Photophosphorylation [b] Oxidative phosphorylation
[c] Phosphorylation [d] Substrate level phosphorylation
11. The reaction centre of PS-I and PS-II respectively:
[a] P680 and P700 [b] P840 and P890 [c] P700 and P680 [d] P600 and P680
12. Which of the following gets reduced during Z-scheme?
[a] NADP+ [b] NAD+ [c] NADPH + H+ [d] NADH + H+
13. Chemiosmotic hypothesis:
[a] Was given by Peter Mitchel
[b] Is applicable for both mitochondrial and chloroplastic ATP synthesis
[c] In chloroplast deals with ATP synthesis which is linked to the development of a proton gradient across the
thylakoid membrane
[d] Is related to all of the above
14. Which of the following minerals are important for photolysis of water?
[a] Zn, Ca, Fe [b] Mn, Ca, Cl [c] Ca, Fe, I [d] Mn, Ca, I
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PHOTOSYNTHESIS IN HIGHER PLANTS (COMPLETE CHAPTER) ASSIGNMENT
15. When O2 is released as a result of photosynthesis, it is a direct by-product of:
[a] Splitting of water molecules [b] Chemiosmosis
[c] The electron transfer system of PS-I [d] The electron transfer system of PS-II
16. In the thylakoid membrane, what is the main role of the antenna pigment molecules?
[a] Synthesis ATP from ADP and Pi [b] Harvest photons and transfer light energy to P680
[c] Transfer electrons to ferredoxin [d] Concentrate photons within the stroma
17. Assume that thylakoid is punctured so that the interior of the thylakoid is no longer separated from the stroma.
This damage will have the most direct effect on which of the following processes?
[a] Synthesis of ATP [b] The absorption of light energy by chlorophyll
[c] Flow of electrons [d] Oxidation of NADPH + H+
18. In plants cells, synthesis of ATP by the chemiosmotic mechanism occur during:
[a] Photosynthesis only [b] Respiration only
[c] Both photosynthesis and respiration [d] Photorespiration only
19. Which of the following is true for cyclic electron transport in the thylakoid membrane?
[a] Acts as a backup for ATP synthesis [b] Both PS-I and PS-II are involved
[c] Operates at a very high light intensity [d] Is related to photolysis of water
20. The first step in light reaction of photosynthesis is:
[a] Photophosphorylation [b] Photolysis of water
[c] Excitation of chlorophyll by sunlight [d] Synthesis of NADPH + H+
21. The NADPH + H+ required for Calvin cycle comes from:
[a] Reactions initiated in the PS-I [b] Reactions initiated in the PS-II
[c] Photophosphorylation [d] Oxidative decarboxylation
22. Which of the following is incorrect?
[a] CF0 is embedded in the thylakoid membrane and forms a transmembrane channel whereas CF1 protrudes on
the outer surface of the thylakoid membrane towards stroma side
[b] The breakdown of the proton gradient across thylakoid membrane provides enough energy to cause a
conformational change in the CF1 particle of ATPsynthase to form ATP
[c] Chemiosmosis requires a proton pump but not a facilitated diffusion channel
[d] Biosynthetic phase of photosynthesis occur both in light and dark
23. The Absorption of sunlight energy causes:
[a] Oxidation of chlorophyll [b] Reduction of chlorophyll
[c] Degradation of all the chlorophyll [d] Damage of accessory pigments
24. Dark reaction of photosynthesis are:
[a] Not directly light driven [b] Dependent on the products of light reaction
[c] Directly driven by light [d] Both (a) and (b)
25. If the oxygen of CO2 is radiolabelled and provided to the plant, then which of following will show radioactivity in
the cells exposed to sunlight?
[a] Hexose [b] Chlorophyll [c] O2 [d] Both (b) and (c)
26. The biosynthetic phase:
[a] Does not directly depend on the present of light [b] Is dependent on the supply of ATP and NADPH + H+
[c] Requires CO2 and H2O [d] Requires all of the above
27. The phylogenetic distribution of the enzyme RuBisCO is limited to:
[a] C3 plants only [b] C3 and C4 plants
[c] All photosynthetic eukaryotes [d] All known photoautrophs, both bacterial and eukaryotic
28. The most crucial step of Calvin cycle is:
[a] Decarboxylation [b] Carboxylation [c] Regeneration [d] Reduction
29. To remove one molecule of glucose from Calvin cycle, _____________ turns of the cycle are required:
[a] 6 [b] 4 [c] 2 [d] 5
30. Which of the following is important to continue Calvin cycle in an uninterrupted manner?
[a] Oxidation of CO2 [b] Regeneration of the CO2 acceptor
[c] Formation of hexose [d] Release of CO2
31. If the carbon atom of the incoming CO2 molecule is labelled with a radioactive isotope of carbon, which organic
molecule will first show the radioactivity?
[a] 3-PGA [b] 1,3-bisphosphoglyceric acid [c] RuBP [d] Glyceraldehyde-3-phosphate
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PHOTOSYNTHESIS IN HIGHER PLANTS (COMPLETE CHAPTER) ASSIGNMENT
32. RuBisCO :
[a] Can bind CO2 but not O2 [b] Is the least abundant enzyme on Earth
[c] With respect to binding of CO2 and O2 is a non-competitive action
[d] Has a much greater affinity for CO2 than for O2
33. Compared to C3 plants, C4 plants:
[a] Can continue to fix CO2 even at relatively low CO2 concentrations
[b] Have higher rates of photorespiration [c] Grow better in cool and moist condition
[d] Do not use RuBisCO for CO2 fixation
34. In C4 plants sugar is synthesised in:
[a] Mesophyll cell [b] Bundle sheath cell [c] Spongy mesophyll only [d] Palisade mesophyll only
35. Which of the following is wrong w.r.t. C4 plants?
[a] Bundle sheath cells present in the leaf contain agranal chloroplast
[b] Bundle sheath cells of leaf possess granal chloroplast
[c] PEP-carboxylase occur in mesophyll cells
[d] Decarboxylation occurs in bundle sheath cells
36. Extrusion of electron from reaction centre of PS II leaves a hole which is filled by electron released from
[a] H2O [b] CO2 [c] Chlorophyll [d] Light
37. Assimilatory power is
[a] NADPH [b] ATP [c] ATP and NADPH [d] FADH2
38. The first product of C4 pathway is
[a] OAA [b] PGA [c] PGAL [d] DHAP
39. Which one is water soluble
[a] Phycobilin [b] Carotene [c] Xanthophyll [d] Chlorophyll
40. In photorespiration the substrate is
[a] Phosphoenol pyruvic acid ATP [b] Ribulose bisphosphate
[c] ATP [d] RuBisCO
41. The essential element required for water splitting in photosynthesis leading to O2 evolution is
[a] Mo [b] Mn [c] Mg [d] K
42. Photo-oxidation of water results in the formation of
[a] H+, O2, ATP [b] H+, O2, e–, ATP [c] H+, O2, e– [d] None of the above
43. Which colour of spectrum is least effective in photosynthesis?
[a] Green [b] Red [c] Blue [d] Violet
44. Last electron acceptor of PSI is
[a] Ferredoxin [b] Cyt b6 [c] Plastocyanin [d] Plastoquinone
45. Which one of the following is essential for photolysis of water
[a] Copper [b] Boron [c] Manganese [d] Zinc
46. PEP is primary CO2 acceptor in:
[a] C4 plants [b] C3 plants [c] C2 plants [d] Both C3 and C4 plants
Given below are assertion and reason, mark the correct choice from given options.
[a] Assertion and reason both are true and the Reason is correct explanation of assertion.
[b] Assertion and reason both are true but reason is not correct explanation of assertion.
[c] Assertion is true but reason is wrong.
[d] Assertion and reason both are wrong.
47. Assertion: All living forms on earth depend on sunlight for energy.
Reason: The use of energy from sunlight by plants during photosynthesis is the basic of life on earth.
48. Assertion: Photosystem I (PS–I) and Photosystem II (PS-II) are part of light harvesting complexes (LHC).
Reason: Photosystem I (PS-I) and Photosynthesis first appeared in some eubacterial species.
49. Assertion: Cyclic pathway of photosynthesis first appeared in some eubacterial species.
Reason: Oxygen started accumulating in the atmosphere after the non-cyclic pathway of photosynthesis
evolved.
50. Assertion: Splitting of the water molecule takes place on the inner side of the thylakoid membrane.
Reason: Protons or hydrogen ions that are produced by the splitting of water accumulate within the lumen
of the thylakoids.
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PHOTOSYNTHESIS IN HIGHER PLANTS (COMPLETE CHAPTER) ASSIGNMENT
51. The assimilatory powers of the plant synthesised during light reaction is/are :
[a] NADPH + H+ [b] ATP [c] Electrons [d] Both a and b
52. Which of the following is correct for chlorophyll-a molecule?
(i) It is soluble in petroleum ether and shows maximum absorption peak at blue and red regions of the visible
spectrum
(ii) Its molecule formula is C55H70O6N4Mg
(iii) It is the main photosynthetic pigment
(iv) It acts as a reaction centre during light reaction
(v) It is a bright blue-green pigment
Options:
[a] (i), (iii), (iv) and (v)
[b] (ii), (iii) and (iv)
[c] (i) only
[d] (i) and (ii) only
53. The water splitting complex is:
[a] Associated with the PS-II
[b] Located on the inner side of the thylakoid membrane
[c] May remain associated with PS-I
[d] More than one option is correct
54. During photolysis of water, protons and O2 formed are initially released in the:
[a] Intermembrane space of chloroplast [b] Thylakoid lumen
[c] Stroma [d] Both (a) and (b)
55. The phenomenon of ATP synthesis with the help of sunlight in plants is called:
[a] Photophosphorylation [b] Oxidative phosphorylation
[c] Phosphorylation [d] Substrate level phosphorylation
56. The reaction centre of PS-I and PS-II are respectively :
[a] P680 and P700 [b] P840 and P890 [c] P700 and P680 [d]P600 and P680
57. Which of the following is correct?
[a] PS-I is involved in non-cyclic photophosphorylation but not cyclic photophosphorylation
[b] PS-II is involved in both non-cycle as+ well as cyclic photophosphorylation
[c] PS-II is involved only in non-cyclic oxidative phosphorylation
[d] PS-I is involved in both non-cyclic as well as cyclic photophosphorylation
58. Chemiosmotic hypothesis :
[a] Was given by Peter Mitchel
[b] Is applicable for both mitochondrial and chloroplastic ATP synthesis
[c] In chloroplast deals with ATP synthesis which is linked to development of a proton gradient across the
thylakoid membrane
[d] Is related to all of the above
59. The breakdown of proton gradient developed during chemiosmosis results in the release of :
[a] H2O [b] CO2
[c] O2 [d] Energy for coupling reaction
60. Given below is the reaction of photolysis of water :
2H2O 4H+ + O2 + 4e–
Which of the following is correct for the above reaction?
[a] Water splitting complex is associated with the P700
[b] Z-scheme is not associated with the above reaction
[c] This reaction occurs in the thylakoid membrane towards lumen side
[d] This reaction occurs in the thylakoid membrane towards stroma side
61. When O2 is released as a result of photosynthesis, it is a direct by-product of :
[a] Splitting of water molecules [b] Chemiosmosis
[c] The electron transfer system of PS-I [d] The electron transfer system of PS-II
62. A plant has a unique photosynthetic pigment. The leaves of this plant appear to be reddish-yellow.
What wavelengths of visible light are being absorbed by this pigment?
[a] Red and yellow [b] Blue and violet [c] Green and yellow [d] Blue and yellow
63. In the thylakoid membrane, what is the main role of the antenna pigment molecules?
[a] Synthesis ATP from ADP and Pi [b] Harvest photons and transfer light energy to P680
[c] Transfer electrons to ferredoxin [d] Concentrate photons within the stroma
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PHOTOSYNTHESIS IN HIGHER PLANTS (COMPLETE CHAPTER) ASSIGNMENT
64. Assume that thylakoid is punctured so that the interior of the thylakoid is no longer separated from the stroma.
This damage will have the most direct effect on which of the following processes?
[a] Synthesis of ATP
[b] The absorption of light energy by chlorophyll
[c] Flow of electrons
[d] Oxidation of NADPH + H+
65. Suppose the interior of the thylakoids (lumen) of isolated chloroplasts were made acidic and then transferred in
dark to a pH = 8 solution. What would be likely to happen?
[a] The isolated chloroplast will make ATP
[b] The Calvin cycle will be activated
[c] Cyclic photophosphorylation will occur
[d] The isolated chloroplast will reduce NADP+ to NADPH + H+
66. The splitting of CO2 to form O2 and carbon compounds occur during :
[a] Photosynthesis [b] Respiration
[c] Both photosynthesis and respiration [d] Neither photosynthesis nor respiration
67. The first step in light reaction of photosynthesis is :
[a] Photophosphorylation [b] Photolysis of water
[c] Excitation of chlorophyll by sunlight [d] Synthesis of NADPH + H+
68. In a plant leaf, the reactions that produce NADH + H+ occur in :
[a] The Calvin cycle alone
[b] Neither light reaction nor Calvin cycle
[c] Both light reaction and Calvin cycle
[d] Occur in chloroplast, but is not a part of photosynthesis
69. The NADPH + H+ required for Calvin cycle comes from :
[a] Reactions initiated in the PS-I [b] Reactions initiated in the PS-II
[c] Photophosphorylation [d] Oxidative decarboxylation
70. Which of the following is incorrect?
[a] CF0 is embedded in the thylakoid membrane and forms a transmembrane channel whereas CF1 protrudes on
the outer surface of the thylakoid membrane towards stroma side
[b] The breakdown of the proton gradient across thylakoid membrane provides enough energy to cause a
conformational change in the CF1 particle of ATPsynthase to form ATP
[c] Chemiosmosis requires a proton pump but not a facilitated diffusion channel
[d] Biosynthetic phase of photosynthesis occur both in light and dark
71. The Absorption of sunlight energy causes:
[a] Oxidation of chlorophyll [b] Reduction of chlorophyll
[c] Degradation of all the chlorophyll [d] Damage of accessory pigments
72. Dark reaction of photosynthesis are:
[a] Not directly light driven
[b] Dependent on the products of light reaction
[c] Directly driven by light
[d] Both (a) and (b)
73. If the oxygen of CO2 is radiolabelled and provided to the plant, then which of following will show radioactivity in
the cells exposed to sunlight?
[a] Hexose [b] Chlorophyll [c] O2 [d] Both (b) and (c)
74. The biosynthetic phase:
[a] Does not directly depend on the present of light
[b] Is dependent on the supply of ATP and NADPH + H+
[c] Requires CO2 and H2O
[d] Requires all of the above
75. The phylogenetic distribution of the enzyme RuBisCO is limited to:
[a] C3 plants only
[b] C3 and C4 plants
[c] All photosynthetic eukaryotes
[d] All known photoautrophs, both bacterial and eukaryotic
76. The most crucial step of Calvin cycle is:
[a] Decarboxylation [b] Carboxylation [c] Regeneration [d] Reduction
77. To remove one molecule of glucose from Calvin cycle, _____________ turns of the cycle are required:
[a] 6 [b] 4 [c] 2 [d] 5
78. Which of the following is important to continue Calvin cycle in an uninterrupted manner?
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PHOTOSYNTHESIS IN HIGHER PLANTS (COMPLETE CHAPTER) ASSIGNMENT
[a] Oxidation of CO2 [b] Regeneration of the CO2 acceptor
[c] Formation of hexose [d] Release of CO2
79. If the carbon atom of the incoming CO2 molecule is labelled with a radioactive isotope of carbon, which organic
molecule will first show the radioactivity?
[a] 3-PGA [b] 1,3-bisphosphoglyceric acid
[c] RuBP [d] Glyceraldehyde-3-phosphate
80. RuBisCO :
[a] Can bind CO2 but not O2
[b] Is the least abundant enzyme on Earth
[c] With respect to binding of CO2 and O2 is a non-competitive action
[d] Has a much greater affinity for CO2 than for O2
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PHOTOSYNTHESIS IN HIGHER PLANTS (COMPLETE CHAPTER) ASSIGNMENT
ANSWERS
Assignment
1 A 11 C 21 B 31 A 41 A 51 D 61 A 71 A
2 C 12 A 22 C 32 D 42 D 52 A 62 B 72 D
3 D 13 D 23 A 33 A 43 A 53 D 63 B 73 A
4 A 14 B 24 D 34 B 44 B 54 B 64 A 74 D
5 D 15 A 25 A 35 B 45 B 55 A 65 A 75 D
6 A 16 B 26 D 36 A 46 A 56 C 66 D 76 B
7 A 17 A 27 D 37 C 47 A 57 D 67 C 77 A
8 D 18 C 28 B 38 A 48 C 58 D 68 B 78 B
9 B 19 A 29 A 39 A 49 B 59 D 69 B 79 A
10 A 20 C 30 B 40 B 50 B 60 C 70 C 80 D
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