BIOCHEMISTRY ASSIGNMENT No.
Molarity, Molality & Normality
1. Molarity (M)
Number of moles of solute dissolved in one litre of solution. It changes with temperature.
Formula: M = moles of solute / volume of solution (L)
Unit: mol/L
Example: If 0.5 mol NaCl (29.22 g) is dissolved in water to make 500 mL solution → 1 M NaCl
solution.
2. Molality (m)
Number of moles of solute dissolved in one kilogram of solvent. It does not depend on temperature.
Formula: m = moles of solute / kg of solvent
Unit: mol/kg
Example: Dissolve 90 g glucose in 0.5 kg water → 1 m glucose solution.
3. Normality (N)
Number of gram equivalents of solute present in one litre of solution.
Formula: N = M × n-factor
Example: 1 M H3PO4 can behave as 3 N if it donates three H■ ions.
Property Molarity (M) Molality (m) Normality (N)
Denominator Volume of solution Mass of solvent Volume of solution
Unit mol/L mol/kg Eq/L
Temperature Effect Dependent Independent Dependent
BIOCHEMISTRY ASSIGNMENT No. 2
Reactions of Glucose, Sucrose, Maltose & Lactose
1. Glucose (C6H12O6)
Monosaccharide and an important source of energy.
Respiration Reaction:
C6H12O6 + 6O2 → 6CO2 + 6H2O + Energy
2. Sucrose (C12H22O11)
Disaccharide composed of glucose and fructose.
Hydrolysis:
C12H22O11 + H2O → Glucose + Fructose (with enzyme sucrase)
3. Maltose (C12H22O11)
Formed during starch digestion.
Hydrolysis:
Maltose + H2O → 2 Glucose (enzyme: maltase)
4. Lactose (C12H22O11)
Milk sugar composed of glucose and galactose.
Hydrolysis:
Lactose + H2O → Glucose + Galactose (enzyme: lactase)
Property Glucose Sucrose Maltose Lactose
Type Mono Di Di Di
Reducing Sugar Yes No Yes Yes
Hydrolysis Product — Glu+Fru 2 Glu Gal+Glu