Head to [Link].
com for more awesome resources
Cambridge (CIE) A Level Biology Your notes
Classification
Contents
Definitions of Species
The Three Domains: Archaea, Bacteria & Eukarya
Eukarya
Kingdoms
Viruses (AL)
Page 1 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Definitions of Species
Your notes
Definitions of Species
Scientists have been classifying organisms into species for hundreds of years, in order to investigate
the diversity of life that exists today and in the past
There is difficulty in determining whether new organisms discovered belong to an existing species, or a
new one
This is because the most widely accepted definition of a species is:
A group of organisms with similar morphological and physiological features that able to breed
together and produce fertile offspring
This is the biological species concept, and is reliant on determining whether interbreeding produces
fertile offspring - this is difficult and time-consuming to determine in practice
However there are other discriminating factors that scientists can use to group similar organisms
together
Morphological Species Concept
In the past, most scientists described organisms by their physical features (morphology) as these can
be more easily observed
They group together organisms that share many physical features that distinguish them from other
species
This is the morphological species concept
Ecological Species Concept
When there is a population of similar organisms living in the same area at the same time, they can be
described as an ecological species
This is the ecological species concept
Naming species
Species are often given common names, but in order to avoid confusion about what group of
organisms scientists are referring to, all species are given a two-part scientific name using the binomial
system
This naming convention was developed and established by the Swedish scientist Carl Linnaeus in the
18th Century
Page 2 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
The first part of the name is the genus that the species belongs to; this is a group of very similar
organisms
Your notes
The second part of the name is specific and unique to a single group of organisms that are identified as
a species (and occasionally there may be a third name)
The binomial name is always italicised in writing (or underlined if it is not possible to italicise)
For example:
The most commonly known yeast is Saccharomyces cerevisiae
It is common to abbreviate the genus name: S. cerevisiae
Saccharomyces paradoxus is another species of that is a member of the same genus as
cerevisiae
Page 3 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
The Three Domains: Archaea, Bacteria & Eukarya
Your notes
The Three Domains: Archaea, Bacteria & Eukarya
Taxonomy is the practice of biological classification
It involves placing organisms into a series of categories or taxa
By grouping organisms into taxa it can make it easier to see evolutionary relationships between
organisms
There are several different ranks or levels within the hierarchical classification system used in biology
The highest rank is the domain
Cell type has a major role in the classification of organisms into the three domains; but do not confuse
cell types and domain
Prokaryotic cells are easily distinguishable in that they lack a nucleus
Eukaryotic cells have compartmentalised structures, with at least their genetic material
segregated from the rest of the cell in a nucleus
Based upon molecular analysis of RNA genes in particular, scientists have realised that using cell type
to classify organisms is insufficient, and that prokaryotes could be divided into two separate groups
(domains)
The three domains are:
Archaea (prokaryotes)
Bacteria (prokaryotes)
Eukarya (eukaryotes)
The Three Domains Diagram
Page 4 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Your notes
The taxonomic classification system with the three domains
Archaea
Organisms within this domain are sometimes referred to as the extremophile prokaryotes
Archaea were first discovered living in extreme environments, but not all archaea do
Archael cells have no nucleus (and so are prokaryotic)
They were initially classified as bacteria until several unique properties were discovered that separated
them from known bacteria, including:
Unique lipids being found in the membranes of their cells
No peptidoglycan in their cell walls
Ribosomal structure (particularly that of the small subunit) are more similar to the eukaryotic
ribosome than that of the bacteria
Archaea have a similar size range as bacteria (and in many ways metabolism is similar between the two
groups)
DNA transcription is more similar to that of eukaryotes
Page 5 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Example: Halobacterium salinarum are a species of the archaea domain that can be found in
environments with high salt concentrations like the Dead Sea
Your notes
Bacteria
These are organisms that have prokaryotic cells which contain no nucleus
They vary in size over a wide range: the smallest are bigger than the largest known-viruses and the
largest are smaller that the smallest known single-celled eukaryotes
Bacterial cells divide by binary fission
Example: Streptococcus pneumoniae is a bacterial species that causes pneumonia
Janice Haney Carr, Public domain, via Wikimedia Commons
Streptococcus pneumoniae
Eukarya
Organisms that have eukaryotic cells with nuclei and membrane-bound organelles are placed in this
domain
Page 6 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
They vary massively in size from single-celled organisms several micrometres across to large
multicellular organisms many-metres in size, such as blue whales
Your notes
Eukaryotic cells divide by mitosis
Eukaryotes can reproduce sexually or asexually
Example: Canis lupus also known as wolves
Canis lupus
User:Mas3cf, CC BY-SA 4.0, via Wikimedia Commons
Examiner Tips and Tricks
Page 7 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
It might be worth refreshing your knowledge on the defining features of prokaryotic and eukaryotic
cells before tackling this new topic!
Your notes
Differences between Archaea & Bacteria
Domains are the highest taxonomic rank that exist within the hierarchical classification system of
organisms
Initially, all organisms within the Archaea domain were classified as Bacteria
Then several unique features possessed by Archaea were discovered that separated them from both
Bacteria and Eukarya
The main differences between Archaea and Bacteria are seen in:
Membrane lipids
Ribosomal RNA
Cell wall composition
Membrane lipids
The membrane lipids found in the cells of Archaea organisms are completely unique
They are not found in any bacterial or eukaryotic cells
The membrane lipids of Archaea consist of branched hydrocarbon chains bonded to glycerol by ether
linkages
The membrane lipids of Bacteria consist of unbranched hydrocarbon chains bonded to glycerol by
ester linkages
Ribosomal RNA
Both Archaea and Bacteria possess 70S ribosomes
The 70S ribosomes in Archaea possess a smaller subunit that is more similar to the subunit found in
Eukaryotic ribosomes than subunits in Bacterial ribosomes
The base sequences of ribosomal RNA in Archaea show more similarity to the rRNA of Eukarya than
Bacteria
The primary structure of ribosome proteins in Archaea show more similarity to the ribosome
proteins in Eukarya than Bacteria
Composition of cell walls
Organisms from the Bacteria domain have cells that always possess cell walls with peptidoglycan
Page 8 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Organisms from the Archaea domain also have cells that always possess cell walls, however these do
not contain peptidoglycan
Characteristics & Features of the 3 Domains Your notes
Feature Archaea Bacteria Eukaryotes
Cell type Prokaryotic Prokaryotic Eukaryotic
Chromosome Circular Circular Linear chromosomes + circular
mtDNA and cpDNA
Cell membrane Glycerol - ether lipids Glycerol - ester Glycerol - ester lipids
lipids lipids
Ribosomes 70S ribosomes but small 70S ribosomes Large 80S ribosomes in the
subunit is more similar to cytosol and 70S ribosomes in
eukaryotic ribosomes mitochondria and chloroplasts
Cell walls Always present (without Always present Sometimes present (without
peptidoglycan) (with peptidoglycan)
peptidoglycan)
Histones Yes No Yes
Introns Sometimes Rarely Yes
Page 9 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Eukarya
Your notes
Eukarya
The hierarchical classification system of organisms in biology is used to organise and group similar
organisms together so that evolutionary relationships between organisms can be more easily
understood
There are several taxonomic ranks that exist
Species is the lowest taxonomic rank in the system
Similar species can be grouped in a genus
Similar genuses can be grouped in a family
Similar families can be grouped into an order
Similar orders can be grouped into a class
Similar classes can be grouped into a phylum
Similar phyla can be grouped into a kingdom
Similar kingdoms can be grouped into a domain
Domains are the highest taxonomic rank in the system
There are a few different mnemonics that exist to help you remember the different ranks in the
taxonomic classification system. You can always make up your own but the one below is super helpful!
The first letters of all the different ranks below the domains can be remembered as:
Kings Play Chess On Fancy Gold Squares
Kingdom Phylum Class Order Family Genus Species
The Hierarchical Classification System Diagram
Page 10 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Your notes
The hierarchical classification system - The higher ranks contain more organisms with less similarity
between them. The lower ranks contain fewer organisms with more similarity between them
Classification of an Organism in the Eukarya Domain
Just like the other domains, Eukarya contains the taxonomic hierarchy of kingdom, phylum, class,
order, family, genus and species
Page 11 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Your notes
The classification system is organised within the eukarya domain - Note there are missing groups at
each rank
A wolf is an example of an organism in the Eukarya domain
It can be classified further into its kingdom, phylum, class, order, genus and species
A wolf belongs to the following taxonomic groups:
Domain: Eukarya
Kingdom: Animalia
Phylum: Chordata
Class: Mammalia
Order: Carnivora
Family: Canidae
Page 12 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Genus: Canis
Species: lupus Your notes
The classification of a wolf (Canis lupus)
The Hibiscus rosa-sinensis is another example of of an organism in the Eukarya domain
It is a colourful flowering plant
It belongs to the following taxonomic groups:
Domain: Eukarya
Kingdom: Plantae
Phylum: Angiospermae
Class: Dicotyledonae
Order: Malvales
Page 13 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Family: Malvaceae
Genus: Hibiscus Your notes
Species: rosa-sinensis
Hibiscus rosa-sinensis
Dinkun Chen, CC BY-SA 4.0, via Wikimedia Commons
A Classification table
Taxonomic rank Wolf Hibiscus
Domain Eukaryote Eukaryote
Kingdom Animalia Plantae
Page 14 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Phylum Chordata Angiospermae
Your notes
Class Mammalia Dicotyledonae
Order Carnivora Malvales
Family Canidae Malvaceae
Genus Canis Hibiscus
Species lupus rosa-sinensis
Examiner Tips and Tricks
The Latinised name of a species always consists of two words: the genus and species. This means
when provided with the Latin name of a species you are automatically provided with information
about the last two taxonomic ranks that the organism belongs to. Remember this when being asked
to show or explain the classification of an organism in the exam.
Page 15 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Kingdoms
Your notes
Kingdoms
The domain Eukarya can be divided into 4 kingdoms:
Protoctista
Fungi
Plantae (plants)
Animalia (animals)
Organisms from each of the four kingdoms have distinct characteristics and features
Although they share similarities:
They have cells with membrane-bound nuclei separating genetic material from the cytoplasm
They have compartmentalisation within their cells as a result of the presence of other organelles
The Four Kingdoms of Eukaryotes Diagram
The four kingdoms within the Eukarya domain: protoctista, fungi, plantae and animalia
Kingdom Protoctista
Page 16 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
All Protoctista are eukaryotic, and this broad group of cellular life encompasses all eukaryotic cells
that do not belong to the other three eukaryotic kingdoms
Your notes
Members of this kingdom show great diversity in all aspects of life including structure, life cycle,
feeding and trophic levels and well as modes of locomotion
Protoctists can exist as single-celled organisms or as a group of similar cells
A group of Protoctista known as protozoa possess cells similar to animal cells
Their cells have no cell wall
Another group of Protoctista known as algae possess cells similar to plant cells
Their cells have cellulose cell walls and chloroplasts
Stentor roeseli is a protoctist that has flagella all over its body which help it feed and move
Stentor roeseli
Public domain, via Wikimedia Commons
Kingdom Fungi
Page 17 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
The oldest organism in the world is thought to be a fungus aged somewhere between 1500 - 10,000
years old
Your notes
All fungi are eukaryotic cells
The cells of fungi:
Possess non-cellulose cell walls (often made of the polysaccharides chitin and glucans)
Don’t have cilia
Fungi are heterotrophs:
They use organic compounds made by other organisms as their source of energy and molecules
for metabolism
They obtain this energy and carbon by digesting dead/decaying matter extracellularly or from
being parasites on living organisms
Fungi reproduce using spores that disperse onto the ground nearby
Fungi have a simple body form:
They can be unicellular (like the common baker’s yeast Saccharomyces cerevisiae
Some consist of long threads called hyphae that grow from the main fungus body (mycelium)
Larger fungi possess fruiting bodies that release large numbers of spores
The mould found on bread is actually a range of species of fungus
Examples include Rhizopus stolonifer, and Penicillium species
Page 18 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Your notes
Bread mould, showing various species growing
Veronidae, CC BY-SA 3.0 via Wikimedia Commons
Fungal Structure Diagram
Page 19 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Your notes
The structure of a fungus with its hyphae, mycelium and fruiting bodies
Kingdom Plantae
Plants are multicellular eukaryotic organisms
Plant cells:
All have cell walls composed of cellulose
Possess large (and usually permanent) vacuoles that provide structural support
Are able to differentiate into specialised cells to form tissues and organs
Possess chloroplasts that enable photosynthesis (not all plant cells have chloroplasts)
Can sometimes have flagella
They are autotrophs
This means they can synthesise their organic compounds and molecules for energy use and
building biomass from inorganic compounds
Page 20 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Plants have complex body forms
They have branching systems above and below the ground Your notes
Bristlecone pines (one species is Pinus longaeva) are found in the USA, it is estimated that some of
them could be 3000 years old
The Gnarly Bristlecone Pine (Pinus longaeva)
Rick Goldwaser from Flagstaff, AZ, USA, CC BY 2.0, via Wikimedia Commons
Kingdom Animalia
Animals are also multicellular eukaryotic organisms
Animal cells:
Are able to differentiate into many different specialised cell types that can form tissues and
organs
Have small temporary vacuoles (for example, lysosomes)
Have no cell walls
Page 21 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Sometimes have cilia
They are heterotrophs
Your notes
They have a wide range of feeding mechanisms
They have a wide range of body forms:
Communication within their complex body forms takes place through a nervous system and
chemical signalling
Blue whales (Balaenoptera musculus) are the largest living animal species
The largest recorded specimen was just under 30 metres in length and 200 tonnes (200 000 kg) in
mass
A blue whale (Balaenoptera musculus)
NMFS Northeast Fisheries Science Center (NOAA), Public domain, via Wikimedia Commons
Page 22 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Viruses (AL)
Your notes
Viruses
Viruses are microorganisms that can only be seen using an electron microscope
They have no cellular structure (and so are acellular) and no metabolism
Viruses hijack the DNA replication machinery in host cells
The energy viruses need for replication is provided by respiration in the host cell
Viruses possess none of the characteristic features used for classifying organisms so they sit outside
of the three-domain classification system
There is a wide-ranging debate as to whether viruses should be classified as ‘living’ or ‘non-living’
based on their inability to carry out the defining features of life outside of a host cell
Classifying viruses by their genetic material
Viruses are classified according to the type of nucleic acid (RNA or DNA) their genome is made from,
and whether it is single-stranded or double-stranded
In cellular organisms like animals and plants, DNA is always double-stranded and RNA is usually
always single-stranded
However, in viruses, DNA and RNA can be either single-stranded or double-stranded
As a result, there are four groups of viruses that exist:
DNA single-stranded viruses
DNA double-stranded viruses
RNA single-stranded viruses (this is the type of genome of SARS-CoV-2, the virus responsible for
the COVID-19 pandemic)
RNA double-stranded viruses
Diversity of Viruses Diagrams
Page 23 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Your notes
The diversity of viral structures
Coronavirus structure
Classification of Viruses table
Page 24 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers
Head to [Link] for more awesome resources
Nucleic Single or double Virus Host Disease
acid stranded Organism
Your notes
DNA Single Canine parvovirus type 2 Dog Canine parvovirus
DNA Double Varicella zoster virus (VZV) Human Chickenpox &
shingles
RNA Single Morbillivirus Human Measles
RNA Double Human immunodeficiency Human AIDS
virus
Page 25 of 25
© 2015-2025 Save My Exams, Ltd. · Revision Notes, Topic Questions, Past Papers