CO2 Incubator (HF151 & HF212)

Operating Instructions
 Recommended Environmental Conditions
In order to extend the machine’s service life, please pay attention to the following factors which may damage
the machine:
• Environmental Conditions;
• Effects of environment, including natural ultraviolet radiation (ie: Direct Sunlight)
• Erosion and abrasion of the protective cover and other safety parts.

• Indoor use;
• Altitude up to 2000m;
• Ambient temperature: +18°C~+30°C;
• Relative humidity: < 80%;
• Operating voltage: 230V, 50/60 Hz;
• Please ensure adequate ventilation in area of use and appropriate sealing measure when performing the
separation of poisonous substances and pathogenic micro-organism cells;
• Ensure there is no vibration and/or a strong draft to influence the performance.
• Ensure there is no dust or flammable and corrosive gas present in the ambient air.
• The setup surface must be firm, level and non-flammable.

Please read this manual carefully when using the instrument for the first time!
The maintenance of the instrument should be carried out by the agents authorized by Biovac.

The following materials should not be used in conjunction with this instrument:

• Flammable and explosive substances;

!
• Strong chemical materials;
• Toxic or radioactive substances,
• Pathogenic microorganisms
!
The CO2 Incubator may only be operated by trained, authorized personnel.
Maintenance work on the unit may only be performed by Vacutec Authorised agents.
Tissue, materials or liquids: Which are highly flammable or potentially explosive;
!
Whose vapours form flammable or explosive mixtures with air;
Which release toxins, May not be used!
The pressure of the CO2 Supply can be adjusted to a range of 0,8-1 bar.
CO2 is a gas which may pose as a potential heath hazard, therefore the unit should be serviced at regular
intervals.
!
Only qualified personnel with specialised equipment may work on supply lines and compressed gas
container, bottles or collective systems in which CO2 is stored for use with the incubator.
N WARN
TURN

!
WARNING - Important operating and/or maintenance instructions. Read the accompaying text carefully
UV THE U
BEFOR

WARNING:

! N TURN OFF
UV THE UV LAMP
BEFORE OPENING

Protective
Glass Hot Surface Risk of Electric Double Pole/ Turn off UV lamp Conductor
Shock Neutral
N Fusing before opening Terminal
WARNING:
TURN OFF
UV THE UV LAMP
BEFORE OPENING

2 N WARNING: CO2 Incubator

TURN OFF
 1: Control Panel (HF-151UV)

Displays and Keys:

1) Main Power Switch:
Main power switch is to turn the unit on/off.
Green Indicator light indicates power on.

2) [ºC] Display:
Displays actual Temp. Value during normal
operation. Press [ºC] key to set Temp. and the
value will be displayed.
Temp. - Related error codes are displayed by
pressing
! the [i] key. Special functions are available
with the [ ] key (see 9.7 Special Function)

3) [%CO2] Display: N

Displays actual CO2 concentration during

WARNING:

normal operation. Press [%CO2] key to set CO2

TURN OFF
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BEFORE OPENING

concentration and the value will be displayed.

CO2 - related error codes are displayed by pressing
! the [i] key. Special functions are available with the
[ ] key. (see 9.7 Special Function)

4) GAS LED Indicator:

N

WARNING:
TURN OFF
The LED flashes when gases are injected.
UV THE UV LAMP
BEFORE OPENING

5) HEAT LED Indicator:

The LED flashes during heating

6) [%CO2] / [ºC] keys:

Set CO2 / Temp Value
!! 7) [ ]/[ ] Keys:
Increase/Decrease values

8) [i] Keys:
Error code Display.
!
9) [ ] Keys:
NN
For special functions enter a function selection.
N

WARNING:
WARNING:
WARNING:
10) Control LED Indicator:
The LED flashes when a special function is
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selected.

11) “Auto-start” function Indicator

12) Over-Temp limit controlling Indicator (red lamp)

comes on when the set Temp. is exceeded.

13) Over Temp. limit control dial

CO2 Incubator 3
 Control Panel (HF-212UV)

Displays and Keys: (Figure 1 - HF151UV)

1) Main Power Switch:
Main power switch is to turn the unit on/off.
Green Indicator light indicates power on.

2) Over-Temp limit controlling Indicator comes on

when the set Temp is exceeded.

3) [i] Keys:
Error code display

4) [%CO2] Keys:
Set CO2 value.

5) [ºC] Keys:
Set Temp value

6) GAS LED Indicator:

The LED flashes when gases are injected.

7) HEAT LED Indicator:

The LED flashes during heating.

8) “Auto-start” function Indicator

9) [ºC] Display:
Displays actual Temp. value during normal
operation. Press [ºC] key to set Temp. and the
value is displayed. Temp. - related error codes are
displayed by! pressing the [i] key. Special features
are available via the [ ] key (see 9.7 Special
Function).
[%CO2] Display: N

Displays actual CO2 concentration during

WARNING:
TURN OFF

normal operation. Press [%CO2] key to set CO2

UV THE UV LAMP
BEFORE OPENING

concentration and the value will be displayed. CO2

- related error codes are displayed by pressing
! the
[i] key. Special functions are available via the [ ]
key (see 9.7 Special Function)
N

10) Control LED Indicator: WARNING:

TURN OFF

The LED flashes when a special function is

UV THE UV LAMP
BEFORE OPENING

selected.
! ! 11) [ ] / [ ] Keys: Increase / Decrease values.
!

12) [ ] Keys: For special functions enter a function

selection.
N

WARNING:
TURN OFF
13) Over Temp. limit controlling dial
THE UV LAMP
UV
BEFORE OPENING

N N

4 CO2 Incubator
WARNING:
 3. Application
The Gassed Incubator can simulate the natural physiological parameters of cell and tissue cultures.

The unit must not be used for treatment of flammable materials that may explode.

4. Abbreviated operating instructions

Factory Settings: Temp. ––– 37.0ºC, %CO2 ––– 0%
Each time at Startup, the following instructions Must be strictly complied to:

a. Open the glass door, fill the reservoir with distilled water, close the door.
b. Turn the “Over-Temp” control knob to 39ºC
c. Connect the gas interface of the unit to the CO2 cylinder.
d. Ensure the power switch at the rear of the unit is “Off ”.
e. Connect the unit to the power supply and turn on the power.
f. The unit will automatically conduct a routine self-test while displaying [%CO2] and [ºC] as [888].
g. Self-test lasts roughly 40 seconds, after which the [%CO2] and [ºC] will display the actual values measured
inside the chamber.
! h. Press [ ] and [ ] together for about 10 seconds, until [%CO2] shows as [0.0] and the “Auto-start” light
comes on, then release both buttons.
i. The unit will begin the “Auto-start” function.
j. Allow the Incubator to stand for 16-24 hours. !

k. Once the Temp. of the unit displays 37.0 ± 0.1ºC, and CO2 displays 0 ± 0.1% CO2, Press !and hold [ ] key
!! while using [ ] [ ] keys to input the desired Temp. set value 3, then release all keys and press [ ] key
again. Finally press [i] key to save the setting. During this process, [%CO2] will show [ .0]. N

N
l. Adjust
N
the CO2 output pressure to 1 bar (0.1MPa).
m.
!!
Press and hold the green [%CO2] key while using [ ] [ ] keys to input the desired CO2 set value (eg.
UV
N
WARNING:
TURN OFF
THE UV LAMP
BEFORE OPENING

WARNING:
TURN OFF

7.0% or 5.0%).
UV THE UV LAMP
BEFORE OPENING

WARNING: WARNING:

UV LAMPn.
TURN OFF TURN OFF
THEUV CO2 gas will be automatically added until the CO2 set value is reached.
THE UV LAMP
BEFORE OPENING
BEFORE OPENING

o. The unit is now ready for working.

N
p. OnceNthe use of the incubator is no longer required, follow the process listed at step m. to set the CO2
value to [ .0]%, and then turn off the power. Open the glass door to allow gases to escape from the inner
chamber and wipe down the chamber.
WARNING:
WARNING:
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5. Description of the Unit

5.1 Structure WARNING:
WARNING:

5.1.1 The outer casing is made of electrolytically galvanized steel sheeting and lacquered white (RAL
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9002).
5.1.2 The control elements are made of plastic.
5.1.3 The inner casing is made of stainless steel.

5.2 Internal Fittings:

The pullout shelves are tilt-proof and adjustable over a height of 50mm. Removal of shelves and carrier
requires no tools and can be removed simultaneously.

5.3 Heating System:

5.3.1 Large-surface heating cables are arranged on the outside of the inner casing, to heat the interior
space. The heating cables are placed beneath the water reservoir, on the ceiling , on the upper part of the
rear wall and in front of the outer door.
5.3.2 Due to the outer door being heated, condensation will not precipitate on the glass door. Therefore
the interior remains clearly visible despite the high humidity. Condensation may form if the outer door is
left open for prolonged periods.

CO2 Incubator 5
 5.3.3 The new normal / slow-heating mode enables the incubator to work properly in different Temp.
conditions. Normal heating mode is recommended.

The special heating system can efficiently operate on 8ºC above ambient Temp.
If the unit is to be operated at lower Temp., you can turn off door heating system and the unit can
operate at 5ºC above ambient Temp. (refer to section 9.7 Special Function 5: Door Heating system).
In this instance, you may experience a little condensation on the corner of the glass door. During
normal operation, the door heating system is “ON” and the auxiliary heating is operating.

5.4 Humidity
5.4.1 The distilled water kept in the reservoir of the unit, evaporates and humidifies the working chamber.
Under normal operating conditions the relative humidity inside the chamber will be ≥ 95%

5.4.2 Because of teh unique heating system, condensation will not precipitate on the chamber ceiling and
glass door. The sidewalls and the rear wall of the chamber serve as appointed condensation surfaces.

Reservoir Volume: 4L distilled water.

Make sure that the water used does not contain any
! chemical agents which may corrode the reservoir. !
5.4.3 Water can be removed from the basin using a pump
a. Prepare teh pipette for removing the water.
b. Remove the shelf inside the incubator.
!
c. Connect one side of the pipette with the output of the electric siphon pump.
d. Press the pump with four suction cups onto the recess at the rear, inside the incubator with the power
cable extending from the top of the pump and plugged into the required power supply.
e. Drain the water until all is removed.
f. Turn off the pump and remove it from the incubator
g. Wipe down the pump and reservoir.
N N

Be careful not WARNING:

to wet the WARNING:
TURN OFF TURN OFF
water pump’s
UV electrical plug
THE UV LAMP UV THE UV LAMP
BEFORE N
OPENING BEFORE OPENING

5.5 Gas Supply:

A suitable connector for the CO2 supply is
located on the rear wall of the unit (figure 3)
WARNING:
TURN OFF
GasUVinlet pressure max. 1 bar
THE UV LAMP
BEFORE OPENING

Please observe the markings of the CO2 connector!

Before entering the chamber, the gas passes a sterile filter where particles larger than 0.3um are retained.
Filter efficiency is 99.998%. A small fan ensures that the gas and in incubator atmosphere are thoroughly
mixed.

6 CO2 Incubator
 5.6 Door Switch:
There is a door switch located behind the glass door. When the glass door is opened, the switch cuts off
the gas supply and the heating system in order to avoid uncontrolled admission of gas or heating of the
chamber. With the glass door open, all displays will flash until the door is shut again. An alarm will be
activated if the door remains open for more than 5 minutes. The outer door can only be closed once the
glass door is locked and air proofed.

5.7 Pressure Compensation:

A pressure compensation vent in the lower section of the chamber rear wall avoids undesirable pressure
build-up during admission of gas. This vent is connected with an overflow (Unique direction) valve on
the rear wall of the unit. The valve impedes unwanted flow of ambient air when the glass door is open.
(figure 4)

The place of installation must be thoroughly

ventilated in order to carry off gas escaping
around the valve. Do Not block this valve!
!
6: Microprocessor Controlled System

6.1 Physical parameter Control:

6.1.1 Temp:
The chamber Temp. is precisely regulated by a microprocessor-based control circuit. Platinum Pt 1000
resistance serve as Temp. sensor. An over-Temp. sensor will automatically cut off the heating system and
trigger an alarm.

6.1.2 CO2 Control: N

a. In order to keep the PH value of the culture media constant at a set value, you need to control the CO2
concentration of the chamber atmosphere. The relevant CO2 concentration depends on the desired PH
value and the content of sodium bicarbonate buffer in the medium.
b. CO2 measurement is based on continuous measurement ofWARNING:the thermal conductivity level of
the chamber atmosphere. This generates an electric signal
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proportional to the CO2
concentration. BEFORE OPENING

6.2 Automatic Calibration of the Measuring System:

AUTO-START Routine
6.2.1 Start-up the unit or changing the Temp. set point requires calibration of the various measuring system.
Your incubator performs this task automatically during the AUTO-START routine.

6.2.2 When both temp. and humidityhave reached teh steady state, the microprocessor recognizes and
performs a fully automatic calibration of the measuring system.

6.2.3 When the Temp. is equals to the ambient, this process requires approx. 15 hours. Prior to AUTO-
START, make sure that the chamber is void of all gases, except air.

ADVICE: You should perform an AUTO-START once every 6 weeks.

6.3 Monitoring Functions:

6.3.1 Door Control:
All actual value displays will flash as soon as the outer door is opened and will continue to do so until
the door is properly closed again. Should the door remain open for more than 5 minutes, the unit will
generate an error message and relative error code can be read.

CO2 Incubator 7
 6.3.2 Error Recognition:
A microprocessor reliably monitors all chamber atmosphere parameters and detects any malfunctions
that might occur. All set values have definite tolerance ranges and an error message will be triggered in
case the actual value is outside the tolerance range.
6.3.3 Tolerance Range
- Temp.: ± 0.5ºC
- CO2 content: ± 1.0% CO2

6.3.4 Error Messages:

a. Errors detected by the microprocessor are assigned to special code numbers. These code numbers will
assist you in identifying the malfunction.
b. When control circuit or displays are affected (e.g. door is open), the LED will come on to remind you
of the malfuntion in the unit. You will hear an alarm sound if the buzzer is connected. Press [i] key to
view the error message. The displays will show [ ––– ] if there are no errors.

6.3.5 Alarm:

If a malfunction occures, you should first press the [i] key to view the error message.
The error message will be deleted if:
• The Set point changes
• You turn the unit off / on.

6.3.6 Error Code Table:

Error Code Possible Reason Recommended Actions

99 - Glass Door is Open Doors are not closed Close doors
100 - Temp. is too Low (SP) Temp. Limit controller is set too Check Temp limit controller
low, observe the indicator Adjustment refer to page 12
101 - Temp. is too High (SP) Ambient Temp. is too high Turn off the door heating switch
(9.7 Function 5)
200 - CO2 too Low (SP) CO2 is not connected Connect gas supply
Refer to installation
CO2 cylinder is empty Replace CO2 cylinder
CO2 inlet pressure is too low Adjust inlet pressure: 1 bar
201 - CO2 too High (SP) CO2 inlet pressure is too high Adjust inlet pressure: 1 bar

6.4 Special Functions:

For adjustment, refer to section 9.7
Use the Function Key, identified by a [ i ] symbol, to select the special functions below.

6.4.1 Buzzer On/Off:

• Function 1:
If the buzzer is turned on you will get an accoustic alarm when errors occur.
Buzzer Off: “Silent” alarm.
Factory Setting: Buzzer ON.

6.4.2 Gas On/Off:

• Function 2:
With the selection of “Gass Off ”, the gas supply is cut off permanently, the green “Gas” LED is illiminated.
Factory Setting: Gas ON, the green “Gas” LED will come on.

8 CO2 Incubator
 6.4.3 CO2 zero-calibration:
• Function 3:
In case the CO2 deviation is too large, this function will assist you in calibrating the CO2 zero point.

6.4.4 Set point Lock-in:

• Function 4:
This function permits you to lock the set points you have entered for Temp. and CO2 so that these values
cannot be changed inadvertently.
Factory Setting: Set point unlocked.

6.4.5 Door heating system on/off

• Function 5:
Normally, the working Temp. is 8ºC higher than the ambient Temp. If the unit is to be operated at lower
Temp., it can be used at only 5ºC above ambient by turning off the “soft” switch in function 5. In this
instance, you might experience a little condensation on the glass door.
Factory Setting: Door heating system “ON”

6.4.6 Heating mode Normal / Slow

• Function 6:
It provides tow heating modes: normal and slow. This enables the indicator to work well under different
Temp. conditions. Slow mode is usually used under high ambient Temp. while Normal heating mode is
recommended.
Factory Setting: Normal mode

6.4.7 Operating Modes: With / Without Water

• Function 7:
If you want to run your unit without water in the reservoir, you need to select this mode prior the start-up
Factory Setting: To be run with water in the reservoir.

7. Over-Temp Protection

7.1 Temp. Limit Controller - (Responds to Temp.)

7.1.1 The unit features an independent electronic Temp. limit controller to protect the incubator, it prevents
the working chamber from over heating. (refer to figure 1 - In compliance with the Thermal Safety Class
3.1 in accordance with DIN 12 880).

7.1.2 In case of a Temp control circuit malfunction where the set Temp is exceeded, the Temp. limit
controller will take over the control function.

7.1.3 Setting it requires a tool (a coin or screwdriver, for example); refer to Section 9.5

7.2 Protecting the unit:

If you set the control knob to 55ºC (limit stop) the Temp. limit controller will protect the incubator.

7.3 Protecting Culture:

7.3.1 The Temp. limit controller will protect teh culture if you set it approx 1-2ºC higher than the Temp.
selected by the microprocessor.

7.3.2 The red indicator will illuminate as soon as the Temp. limit controller responds.

CO2 Incubator 9
 7.3.3 In the case where the Temp. limit controller has taken over the control function, refer to 10.1 Error
Code Table to investigate and determine the cause of the malfunction.

7.4 Operational Test:

At resonable intervals your Temp. limit controller should be tested for proper operation. To run this test, turn
the knob to where is shows approx. 2ºC less than the Temp. indicated on the display. The red indicator
light should come on. We recommend that your authorised operators run this test on a routine basis, e.g.
Prior to a lengthy working process.

8: Location and Installation

8.1 Location
a. Unpack your unit and remove all accessories from the interior.
b It must be located in a dry area where the ambient temperature does not exceed 30ºC
c. Place the unit on a level, non-combustible surface that does not vibrate.
d. The gassed incubator will run with CO2, which is not harmful to the operator when the room is
adequately ventilated.. The gases escape from the back wall of the unit, therefore adequate space must be
allowed around the unit to ensure the gas emmissions are not restricted.
e. The unit should not be placed and operated in areas without adequate ventilation
f. If several units are placed in the same room or the unit is installed under a laboratory bench, additional
ventilation equipment must be installed.
g. For information on gases released during operation, consult the Appendix.

8.2 Postioning Distances:

To ensure adequate ventilation, the required space surrounding the unit should be no less than 50mm left,
right and top, while no less than 120mm between the rear of the unit and any other object or wall.

DO NOT block the overflow valve on the rear wall of the unit. Do not connect a hose to the valve outlet as
this could cause condensation to occur.

8.3 Stacking:
Refer to section 13.1

8.4 Installation, Connection to Gas Supply:

a. The appropriate interface is placed on teh back of the unit. A relative hose is provided with the unit. The
gas required for operation of the unit is to be connected via a pressure reduction valve, set a 1 bar. The
pressure reduction valve will only be supplied on customer’s request.
b. For technical safety reasons, the 1 bar setting must not be changed.

10 CO2 Incubator
 8.5 CO2:
Connect the gas cylinder, with the pressure reduction valve, to the unit.

8.6 Reminder:

The gases must be at least 99.5% pure.

Do not use a CO2 cylinder with a cart.
!
8.7 Connection to Main Power Supply:
a. Prior to connecting the unit to mains, ensure that the power ratings match those stated on the nameplate.
b. Your gased incubator is equipped with a properly grounded power cable.
c. A 6.3 delay-action type fuse is required for mains fusing.

9. Start-Up
The ambient Temp. of the location must be at least 8ºC lower than the Temp. setting. Keep the outer door
and the glass door open.
N
9.1 Operation with Distilled Water:
a. Fill the reservoir with distilled water (at approx. room Temp.) Filling level: 4L
b. Make sure not to spill any of the water when filling the reservoir.
WARNING:

9.2 Operation without Distilled Water: UV

TURN OFF
THE UV LAMP
a. Consult the special function table (section 9.7) and select Function Code 7, “Operating mode w/o
BEFORE OPENING

water” and enter [U.O]

b. Ensure the relative humidity inside the chamber does not change. Only use sealed culture dishes.
c. Open the valve on the gas cylinder.
d. Turn on the main power supply.
1. Green indicator illuminates.
2. The display reads [ 888 ] for approx. 40 seconds
3. Auto-Start Routine.
4. Once Auto-Start completes, the displays will indicate the actual values within the chamber.

9.3 Set Point Adjustment:!

!
a. Use: Set point adjustment [ºC], [CO2], [ ] / [ ] keys.
b. To adjust [ºC], press the relative set point key [ºC]:
c. To adjust [CO2], press the relative set point key [CO2]:
1. The adjusted set point is displayed.
! ! [Link] last digit on the display is flashing.
the [ ] or [ ] key to adjust the desired set point.
d. If you release the set point key, the new set value will be stored and the actural value is displayed again.
N N

9.4 Factory-Settings:
Temp.: 37ºC WARNING:
WARNING:

CO2: 0.0% UV UV
TURN TURN
OFF OFF
THE UV
BEFORE
LAMP
THE UV LAMP
OPENING
BEFORE OPENING

9.5 Adjustment of the Temp. Limit Controller:

N N

Set it approx. 1-2ºC higher than the selected Temp. Refer to section 7 “Over Temp. Protection”
WARNING:
WARNING:
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BEFORE OPENING

CO2 Incubator 11
 9.6 AUTO-START
9.6.1 ATTENTION! the chamber must be void of any gases except air.
! 1. Press
! both the [ ] and [ ] key for approx. 10 seconds.
2. The AUTO-START routine is activated and the CO2 display will reflect [0.0] after closing the door.
3. Gas LED is off
4. Shut the doors.

9.6.2 Reminder:
The unit proceeds to heat to the adjusted Temp. set point and builds up the relative humidity. After
finishing the Auto-start routines.
N N

1. LED AUTO-START is off.

2. Actual values are displayed
WARNING: WARNING:

3. Gas LED is on.

UV
TURN OFF TURN OFF
THEUV
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BEFORE OPENING
BEFORE OPENING

9.6.3 If you input the CO2 set value, gas will be injected until the adjusted set point is reached.

9.6.4 The unit is now ready for work.

9.7 Special Functions:

!
! ! ! key and [ ]/[ ] keys.
9.7.1 Keys to be used: [ ]
! !!
9.7.2 If you press and hold the [ ] key, you can select and set special functions with the [ ]/[ ] keys.
9.7.3 The [ºC] display will reflect the function code, e.g. [ .1]. If you release the [ ] key and then press it
N

again, you will get the status report in the CO2 display.
WARNING:
TURN OFF
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UV THE UV LAMP
BEFORE OPENING

N
WARNING:
TURN OFF
THE UV LAMP

Function Code Temp. Display [ºC] Display [CO2] Function Factory Setting
UV
BEFORE OPENING

WARNING:
TURN OFF
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BEFORE OPENING

1. Buzzer N N
[ 1] [A 1] Buzzer ON Buzzer ON
[A 0] Buzzer OFFN N
2. Gas [ 2] [G 1] Gas ON Gas ON
WARNING:
[G 0]
WARNING:
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TURN
Gas OFF
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3. CO2 zero-calibration [ 3] [ .0] CO2 calibrate

UV UV
BEFORE OPENING
BEFORE OPENING WARNING:
WARNING:
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UV LAMP

4. Set point release [ 4] [S 1] Not Released Released

BEFORE OPENING
BEFORE OPENING

[S 0] Released
5. Door Heater [ 5] [d 1] ON ON
[d 0] OFF
6. Heating mode [ 6] [H 1] Normal Normal
[H 0] Slow
7. Operation mode [ 7] [U 1] With Water With Water
[U 0] Without Water
For more information, refer to section 6.4

9.8 Notes:

9.8.1 Temp.:
If you change the Temp. set point by more than 1ºC, you will have to restart the unit as described in the
chapter for Startup so that the gas detectors will operate with the utmost precision.

9.8.2 CO2 Comparison:

If a precise CO2 comparison measurement should reveal a concentration that differs a lot from the actual
value displayed, you can manually correct the CO2 measuring system while it is running . In this way you
dont need to start the unit all over again.

12 CO2 Incubator
 9.8.3 Reference Example:
Conditions: CO2 display: 7.0% CO2
CO2 measured: 6.2% CO2
!
!
a. Use !the [ ] and the [ ] keys to select function code 3.
b. Release the! [ ] key.
c. Keep pressing the [ ] key again.
N

(1) The Temp. display indicates [ .3]

WARNING:
TURN OFF
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!d.! (2)
! The [%CO ] display indicates [ .0]
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BEFORE OPENING

N
2 WARNING:

Use! the [ ] + [ ]/[ ] key to set the measured concentration in the Temp. display, e.g. [6.2}.
TURN OFF
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BEFORE OPENING

WARNING:
TURN OFF

e. Release the [ ] key.

UV THE UV LAMP
BEFORE OPENING

N
f. Press the [i] key briefly to confirm (to start computation by the microprocessor).
N

g. The CO2 display will indicate the corrected concentration after the computation is completed. If you
WARNING:
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now find that the actual value displayed exceeds the set point, you will have to open the doors for approx.
UV THE UV LAMP
BEFORE OPENING

WARNING:
WARNING:

15 sec. in order to evacuate the excess gas.

TURN OFF
UV THE UV LAMP
TURN OFF
BEFORE OPENING

UV THE UV LAMP
BEFORE OPENING

9.8.4 General NRemarks:

N

a. If your unit will not be in use for quite some time, you must drain the water from the reservoir and
wipe down the chamber surfaces.
WARNING:
WARNING:

UVb. Do not turn the unit on and off continuously.

UV
TURN OFFOFF
TURN
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BEFORE
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OPENING
BEFORE OPENING

c. In order to keep recovery times as short as possible and to create optimum chamber conditions, only
open the door when needed and keep the duration as short as possible.

9.8.5 [i] Key:

The relevant displays will illuminate for any malfunction during operation. If the buzzer is connected, it
will sound symiltaneaously. Press [i] key to get information of the malfunction. The affected display [ –––
] will show the error code. (Refer to error code table, section 10)

10: Error Code Table

10.1 Error code Table:

In order to correct malfunctions as soon as possible in the case of an error, refer to the following table.

Error Code Possible Reason Recommended Action

99 Glass Door is Open Doors are not Properly Closed Close the door
100 Temp. is too low (SP) Temp. limit controller is set too Check and adjust Temp. limit
low. Check the indicator controller
101 Temp. is too high (SP) Ambient Temp. is too high Turn off the door heating switch
(Section 9.7 Function 5)
200 CO2 concentration is too CO2 is not connected Connect gas supply
low (SP) CO2 cylinder is empty Replace CO2 Cylinder
CO2 inlet pressure is too low Adjust inlet pressure: 1 bar
201 CO2 concentration is too CO2 inlet pressure is too high Adjust inlet pressure: 1 bar
high (SP)

* If this does not resolve the error, call Vacutec’s service department for assistance.
SP = Set Point

CO2 Incubator 13
 10.2 Malfunctions which are not displayed:

Malfunction Recommended Action

Unit does not heat up Check set point
Unit does not input gas Check gas LED
Check whether gases are released
Check set point
Buzzer does not sound when an error occurs Check buzzer is turned on,
refer to section 9.7 Special Functions
Set points cannot be changed Set point are locked,
refer to section 9.7 Special Functions
Excessive condensation on glass door Check switch for door heating,
refer to section 9.7 Special Function 5

10.3 Turn off the Unit:

1. Turn off the mains power switch
2. Shut the pressure reduction valve of the gas supply.

11 Disinfection, Cleaning and Checks

11.1 Weekly Checks

1. Check level of distilled water in the reservoir. !
2. Check if the gas supply pressure is at 1 bar

11.2 Disinfection and cleaning of the interior chamber

[Link] the unit off before cleaning the incubator.
2. Open the glass door.
3. Drain the water (refer to section 5.4.3) N

11.3 Reminder
The gas analyzer should only be cleaned with the recommended disinfectants
WARNING:
TURN OFF
UV THE UV LAMP

1. Do not use disinfectants containing more than 10% alcohol!

BEFORE OPENING

2. Prior to calling service for maintenance and repair work, it might be neccessary to clean and disinfect
the unit.

11.4 Maintenance
It is recommended to run an AUTO-START at least once every 2 months to re-calibrate the gas analyzer.

We only guarantee safety of the unit when maintenance, repairing or

modifications are conducted by personnel authorized by Vacutec.
Check your unit at regular intervals to ensure proper operation.
The unit should be inspected anually for safety, leak proof and a service.
!
11.5 UV light
In operation, fungi may develope in the incubator. To protect the unit from contamination and keep
samples pure in the chamber, a UV light can be applied to disinfect the inner chamber.

11.5.1 Location of UV Light

UV light is located on the upper part of the rear wall of the inner case. It is connected to the
microprocessor controller through two special, air tight, water proof, Silastic lamp sockets. The protection

14 CO2 Incubator
 switch (UV light switch) is positioned on the back wall of the incubator and is marked as (UV). Please
keep the switch in off position to avoid accidental triggering of UV light which may destroy samples.
After long periods of use, the lamp may turn black at each end, burning out. In this case, the UV bulb will
need replacing.

DO NOT CHANGE THE UV BULB YOURSELF, this procedure may only be performed by an authorised
Vacutec Service Engineer.

11.5.2 How to use the UV light

If you want to turn on the UV light, two switches need to be activated. One switch is a software switch,
Function 222, the other is a hardware switch found on the rear wall of the incubator.
Steps:
1. At start up, the incubator will perform a routine self-test for roughly 40 seconds. [888] displayed on the
panel will then reflect an actual value.

2. Before starting the UV light disinfecting function, ensure that there are no samples in the incubator!
a. Turn on! the UV light protection switch on the rear wall of the incubator, set [%CO2] to [0%].
!
! !
b. Press and hold [ ] key! and press [ ] or [ ] key simultaneously. Temp. display shows [222], release
[ ] key, then continuously press [ ] key, CO2 displays [0], thus entering the special function 222 (UV
!
!
light Switch), press [ ] key and [ ] key, set [%CO2] to [1], indicating that the UV light is on. Press [i] to
N

N confirm, UV light will illuminate, display panel shows [–––].

WARNING:
N

c. When the UV light is on, do not look directly into the lamp! Turn off the UV light before opening
TURN OFF
UV THE UV LAMP
BEFORE OPENING

N
WARNING: WARNING:

the glass door!

TURN OFF TURN OFF
UV THE UV LAMP UV THE UV LAMP
BEFORE OPENING BEFORE OPENING

WARNING:
TURN OFF
UV THE UV LAMP
BEFORE OPENING

3. After 30 minutes, the disinfection program will end and the UV light will automatically turn off. The
N N

display panel will once again display an actual value. Open the outer and glass doors to let the ozone out
N
of the chamber. Switch the protection switch, at the rear of the incubator, back to OFF.
WARNING:WARNING:
TURN OFFTURN OFF
UV THE
UV
UV LAMP
THE UV LAMP
BEFORE OPENING
BEFORE OPENING

WARNING:
TURN OFF
UV THE UV LAMP
BEFORE OPENING

WARNING:
Cleaning and disinfection of the incubator is to done prior to calling out a maintenance engineer.

In the event that a rust preventative disinfectant with chlorine content is used
to disinfect either the interior or exterior parts of any equipment,
it is necessary to rinse the disinfected parts with distilled water and
wipe down the equipment prior to further use.

CO2 Incubator 15
12 Technical Data

HF 151

Data Unit
DIMENSIONS
Outer Casing (Width) 615 mm
(Height) 855 mm
(Depth) 677 mm
Inner Chamber (Width) 470 mm
(Height) 607 mm
(Depth) 528 mm
VOLUME 151 L
SHELVES
Full-width shelves
3
Max 10
Dimensions Width 423 mm
Depth 445 mm
NET WEIGHT 75 kg
ELECTRICAL DATA
Rated input voltage 230 V
Connected load / rated power 0.70 kW
Rated frequency 50 Hz
FUSE AC250V, F6.3A
TEMP. CONTROL (digital)
Temperature Range ± 5-50 ºC
Holding power at 50ºC 0.1 kW
Spatial Temp. deviation ± 0.4 ºC
DIN 58 945
Temporal Temp. deviation ± 0.1 ºC
DIN 58 945
CO2 CONTROL (digital)
Setting range 0-20 %CO2
Setting accuracy 0.1 %CO2
Time of recovery ca. 1 %CO2/min
Gas supply ca. 3,7 L/min
HUMIDITY
Relative humidity (constant humidity) >95 %
FINISH (Textured Lacquer) 9002 RAL
GAS SUPPLY DATA
Filter in gas pipeline 99.998 %
Filter efficiency at particle size >0.3 um
Purity of gases used min. 99.5 %
Gas inlet pressure 1 Bar
NOISE LEVEL <60 dB (A)
DISTILLED WATER LEVEL 4 L

16 CO2 Incubator
 HF 212

DATA Unit
DIMENSIONS
Outer casing (Width) 910 mm
(Height) 795 mm
(Depth) 763 mm
Inner chamber (Width) 600 mm
(Height) 600 mm
(Depth) 588 mm
VOLUME 212 L
SHELVES
Full-width shelves 3
Max 10
Dimensions Width 590 mm
Depth 510 mm
NET WEIGHT 95 kg
ELECTRICAL DATA
Rated input voltage 230 V
Connected load/rated power 0.80 kW
Rated frequency 50 Hz
FUSE AC250V, F6.3A
TEMP. CONTROL (digital)
Temperature Range ± 5-50 ºC
Holding power at 50ºC 0.1 kW
Spatial Temp. deviation ± 0.4 ºC
DIN 58 945
Temporal Temp. deviation ±0.4 ºC
DIN 58 945
CO2 CONTROL (digital)
Setting range 0-20 %CO2
Setting accuracy 0.1 %CO2
Time of recovery ca. 1 %CO2/min
Gas supply ca. 3,7 L/min
HUMIDITY
Relative humidity (constant humidity) >95 kg
FINISH (Textured Lacquer) 9002 RAL
GAS SUPPLY DATA
Filter in gas pipeline 99.998 %
Filter efficiency at particle size >0.3 um
Purity of gases used min. 99.5 %
Gas inlet pressure 1 Bar
NOISE LEVEL <60 dB
DISTILLED WATER LEVEL 6 L

CO2 Incubator 17
 13 Appendix

13.1 Stacking of Gassed Incubators:

If several units are placed in the same room or if a unit is installed underneath a laboratory bench,
additional ventilation equipment must be provided.

a. One gassed incubator can be placed on top of another with the provided stacking feet.
b. One set includes four stacking feet onto which the upper unit is fixed
c. The 4 stacking feet should be screwed into the holes on the upper part of the unit with the enclosed
self-tapping screws.

13.2 Gas Volume for Normal Operation and Malfunction

Explanation of terms used:
1. Required amount of gas: The amount of gas needed in order to reach the max adjustable set point.
2. Gas consumption: The volume of gas constantly injected to maintain the max adjustable set point.
3. Malfunction: When over pressurisation occurs due the gas exceeding the maximum set point, an
audible alarm will sound accompanied by a visual alarm.
4. Gas flow: With the valve fully open, the volume of gas flowing through the capillaries per hour.

Gas Type Gas inlet Capillaries Gas flow Required Gas Malfunction: Limit
pressure (*4) amount of consumption Escaping Valve
gas (*1) amount of gas
(*3)
CO2 1 bar 0.65mm 222L/h 50L 0.52L/h 444L 5Vm3

18 CO2 Incubator
 13.3 CO2 Consumption and PH Value
CO2 Consumption (Working status of door is closed), shown as Figure 7

Figure 7: CO2 Consumption (working status of door is closed)

PH Value of the culture (necessary for biology cultivating)

PH value of the culture is affected by CO2 concentration in the chamber, figure 8 shows the relations between
PH value and CO2 concentration.

Figure 8: Culture PH value — CO2 concentration

CO2 Incubator 19
 P.O. Box 48488, Roosevelt Park, 2129,
South Africa.
Tel: Jhb 011 475 1823
Dbn 031 301 7617
CT 021 461 9607
Fax. 011 475 1819
email: support@[Link]

20 CO2 Incubator