MICROSCOPE
Microscope
3 Major Parts
(2) Illuminating
Parts & Function ➢ supply & regulate light towards the specimen
➢ provide the light
➔ Mirror - reflects light from external light
Source
➔ Iris Diaphragm - controls the amount of
light that reaches the specimen
- rotating that controls the passage of light
through the stage
- “5” highest “1” lowest
➔ Stage Condenser - collects & focuses the
light from the mirror to the specimen
- gives a sharper image
Microscope
3 Major Parts
Microscope (3) Mechanical
➢ a tool used to see organisms or objects that ➢are used for support & adjusting the different
are too small for our naked eye parts of the microscope
➢EX: Plants cells, animal cells, microorganisms ➔ Draw Tube - holds the ocular
Microscope ➔ Body Tube - holds the eyepiece to the
Compound Microscope nosepiece, to objective lens
➢an optical microscope that ises visible light to ➔ Revolving Nosepiece/Turret - the changing
of objectives
Form an image.
- holds the objectives
➢uses glass lenses to magnify & resolve image
➔ Adjusting Knobs
Microscope
a. Coarse Adjustment Knob - moves the
3 Major Parts
body tube & lower power lenses, close
(1) Magnifying
or farther away from the stage
➢make the specimen look bigger
- focus on scanning
➢ enlarge
b. Fine Adjustment Knob - focus the image
➢ adjust & support the parts
further
➔ Eyepiece/Ocular Lens - is used to see the
- used for focus on oil
the objects
- moves the body tube for focussing the
- where you view/look the magnified image high power lens
- 10x or 15x ➔ Arm - support the body tube & is held
➔ Objective Lenses - the major lenses - connects to the base of the microscope
- 3 or 4 objective lens ➔ Base - supports the entire microscope
- 10X, 40X, 100X ➔ Stage - where slide is placed
- below the nosepiece ➔ Stage Clips - secure/holds the slides
a. Lower Power Objective (10x) - Lowest ➔ Inclination Joint - tilting of the microscope
b. High Power Objective (40x) - Higher ➔ Pillar - supports the other parts
c. Oil Immersion Objective - Highest
MICROSCOPE
Transmission Electron Microscope 1931
History
Nimrud Lens (710 BCE)
➢found in the city of Nimrud
1595 Scanning Electron Microscope 1942
➢ The first compound microscope
➢ Zacharias Janssen (son)
➢ Hans Janssen (father)
Confocal Microscope 1978
Scanning Tunneling Microscope 1981
Antonie Van Leeuwenhoek
➢cloth merchant
➢who made the first microscope that can
Visualize an individual cell
➢ made over 500 prototypes to get the final
version of his microscope which could
visualize 200x magnification
➢ led to the discovery of bacteria, protists,
sperm cells etc
➢ animalcules
Robert Hooke 1635
➢added the features to the microscope (stage &
Illuminator, focus controls)
Light Microscope 1595
Phase Contrast Microscope 1930
MICROSCOPE
Parts