Inatrurunt Molhods NAnalyia BP7oT
hao oromatograbhy Dr. Parjanya Shuhla
Th conupB of aC dopandant on folbuwing Dr. M.P. Singh Clasea
pOromotarA-O
) Relanhon Time CR)- 4 may br dainud as
Ki [Link] im taken by o hampll to show
maximum beak on chromakbqrom 0ttRT injsthin9
Katenhon Hima may bi
ComsicurR d a
toral timu sþend by somplu Pmox
in atahonary phasl ((RE') and
th mobil pha»i (me)
R RMe
R Combluhly dsbandson
oprahma comdiion6 and Tnjatbon point
expwwad in minuts
R
-RE
&) Rabankon Volum (Va)
y4 my hs daina d as K volumu d gas uquwred to
eute about kalf d tKi solute RYough calumn,.
X
X is produ ulenhon imu (R:) and gas low sahe(f)
VAR x f By-Dr. Mohndra Arahap Singh
ColumnEliciany- h gas chromaograbhy
Dr. Parjanya Shukla
thi eicinay o
pRT/omanu column uAualy Dr. M. 9. 5ingh Clases
miasurAd in terms number qd Kiorehcal þlakes
as wll as buighB oqivaunt to thoriHical plaks (HETP)
a) Plals Thaory - Aecoding to plata kovy
th Columo AAd in GC ou considarad ta. ba madi up
e v r a poroUal lays1s known as thioTshcal þlates
During chvomakography i pposed hal he
mobil phase moves over s column hi suhuks parhdu
Rayulibraks b/o .P and s.P.
K soluls and saluent bosng trans/amad rom on
plate to anoker n stap-wisA patern
lolumn oiu'ny con br intrasd by incrzasing tK
numbyr KioTaheal plates
Th ochuol numbr othoTakal platu in o coumn can
b calwlatad -
Thiorahical Plake may b
N 16 R Comiderad as di'atonu/ orAa in
W which somplu sqwlibmum is at/nud
b/w gas and bigud
luhu N Numbar of Kaorabral blakes
RtDiafonu betoean injochom povt and ta
maximum piak hoght (xutanbm Himu)
Peak oidh obtainad rom /3d hviqht o
tks paak up to Ks base inu.
Hight
Height Equivalunt to TkLovehical Plates (HE TP)
dedinsd as Dr. Parjanya Shukla
Dr. M.P. Singh Classes
th igh H column iquwiredfr
forequibibrahorm ot tkhe
SoJufa bitoen mobil and stahmay phasa
HETP
N
whuu L Jangh od h calumn (cm).
o gTAaker Ks [Link] o tRiorahcal plats or amallur ha HETPe
higher i K calumn ollitany.
Plata Rio xplain Ks
efact od paromatara or vonablu on
miqrabon xata os0mplu in quonitabve tams.
bu Cowd nof xplain h dactor xuponaiblu
OT bond broo denin 9.
(b) Rata Thaoy
x Alhough N and HETP desenibos K column aHiusny
tKay do no give any informahm abou fact
Voriabls on bond broodsning and bond apar abon
Von DumBar [Link]. ropoaad tka uahe tkiony to
OVA ComL Ris drowback late kaony
HETP A+/u + Cu
wh A ddy diusion
= Jongitudinal molsudar difluwion
C maw ransar
u AvwTaga qas vaouty or low xate (Cm/Sec)
Dr. Parjanya Shtula
HETP Dr. M. P. Singh Classes
A
B/u
Lintar Velouky CCm/s)
Eddy Diuion (A)orMulhpalh efteedi
As Ke soluse mal ules trovals in difeunt pahs in þacked
coumn Ki mouuls have dluunB idnu hm
Eddy dilusion should bi xuduud to
enhonu column effiuenuy
by ing small unifom siz« parhles
(loo-120 sh) and column wih smaler diomutar (/8 inch)
Longitudinal ditmion (8)
Lanqitudi'nal dilyuion occuTs
soluh mou uls from cmunkatd unu
whun t
oa bond mov to moru dilutt ugion on githtY side.
Lom a column
uponsiblu for bond broadening
eliuany
Column
* oiuany Can bs inaaakad by using
»(omir gas wih hig molseulor wwighl and
UnorJouw Aate
Mn Tromav Cc):-
Mas TronsA7 deined Ks inlunu d h Dr. Parjanya Shukla
&
amaunf and viacoAiy of liqud on s0lid Aupport pr. M. . Singh Clasms
Joo viscosik, Jouo vapour prLBure and
0od Aolubilty or Aomplu should br ud.
[Link] Pralap Sing
Viu -hinupal, Amo. Pro
Kisharpil Ioshituhs o Phamauy
rayaga 0.P. In dia.