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Precision Analysis in Laboratory Methods

This document contains 5 questions from a chemistry tutorial regarding data analysis and calculations. Question 1 asks to calculate the pooled standard deviation from absorbance data from 3 samples measured over several days. Question 2 asks to determine if there is a significant difference in precision between atomic absorption spectrophotometry and a colorimetric method based on calcium concentration data. Question 3 asks if one result from standardization data can be discarded as accidental error. Question 4 asks to determine the correlation coefficient between toxin production and yeast extract concentration based on sample data. Question 5 asks to determine the correlation coefficient between fungal dry weight and toxin amount using the same sample data.

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Jc Jackson
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200 views1 page

Precision Analysis in Laboratory Methods

This document contains 5 questions from a chemistry tutorial regarding data analysis and calculations. Question 1 asks to calculate the pooled standard deviation from absorbance data from 3 samples measured over several days. Question 2 asks to determine if there is a significant difference in precision between atomic absorption spectrophotometry and a colorimetric method based on calcium concentration data. Question 3 asks if one result from standardization data can be discarded as accidental error. Question 4 asks to determine the correlation coefficient between toxin production and yeast extract concentration based on sample data. Question 5 asks to determine the correlation coefficient between fungal dry weight and toxin amount using the same sample data.

Uploaded by

Jc Jackson
Copyright
© All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

AMZ

Tutorial 1

CDB3093

Question 1:
Your laboratory is evaluating the precision of a colorimetric method for creatinine in serum in
which the sample is reacted with alkaline picrate to produce a color. Rather than perform one
set of analyses, several sets with different samples are performed over several days, in order to
get a better estimate of the precision of the method. From the following absorbance data,
calculate the pooled standard deviation.
Day 1 (Sample A)
Day 2 (Sample B)
Day 3 (Sample C)
0.826
0.682
0.751
0.810
0.655
0.702
0.880
0.661
0.699
0.865
0.724
=0.845
=0.666
=0.719
Question 2:
The following replicate calcium determinations on a blood sample using atomic absorption
spectrophotometry (AAS) and a new colorimetric method were reported. Is there a significant
difference in the precision of the two methods?
AAS
Colorimetric
(mg/dL)
(mg/dL)
10.9
9.2
10.1
10.5
10.6
9.7
11.2
11.5
9.7
11.6
10.0
9.3
Mean 10.4
10.1
11.2
Mean 10.4
Question 3:
The following replicate molarities were obtained when standardizing a solution: 0.1067, 0.1071,
0.1066, and 0.1050. Can one of the results be discarded as due to accidental error at the 95%
confidence level?
Question 4:
From the data given below, determine the correlation coefficient between the amount of toxin
produced by a fungus and the percent of yeast extract in the growth medium.
Sample
%Yeast Extract
Toxin (mg)
(a)
1.000
0.487
(b)
0.200
0.260
(c)
0.100
0.195
(d)
0.010
0.007
(e)
0.001
0.002
Question 5:
The cultures described in Problem 40 had the following fungal dry weights: sample (a) 116mg,
(b) 53mg, (c) 37mg, (d) 8mg, and (e) 1mg. Determine the correlation coefficient between the
dry weight and the amount of toxin produced.

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