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Biomass Yield Coefficients in S. cerevisiae

This document contains 4 questions regarding biochemical engineering reactions and stoichiometric calculations. Question 1 involves determining coefficients for the aerobic growth of yeast on ethanol based on a given reaction equation and respiratory quotient. Question 2 involves determining coefficients for the biological denitrification of nitrates based on a given reaction and biomass yield. Question 3 involves determining coefficients for the aerobic degradation of an organic compound by mixed culture based on a given reaction and biomass yield. Question 4 asks to calculate theoretical yields based on a generalized aerobic yeast growth reaction using ethanol as substrate.

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122 views1 page

Biomass Yield Coefficients in S. cerevisiae

This document contains 4 questions regarding biochemical engineering reactions and stoichiometric calculations. Question 1 involves determining coefficients for the aerobic growth of yeast on ethanol based on a given reaction equation and respiratory quotient. Question 2 involves determining coefficients for the biological denitrification of nitrates based on a given reaction and biomass yield. Question 3 involves determining coefficients for the aerobic degradation of an organic compound by mixed culture based on a given reaction and biomass yield. Question 4 asks to calculate theoretical yields based on a generalized aerobic yeast growth reaction using ethanol as substrate.

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tiffanyyy00
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CHE 4171: Biochemical Engineering

TUTOTRIAL QUESTIONS (WEEK 2 T1)


Questions
1. Aerobic growth of S. cerevisiae on ethanol is simply described by the following overall reaction.
C 2 H 5OH + aO2 + bNH 3 c(CH 1.704 N 0.149O0.408 ) + dCO2 + eH 2O
(a) Determine a, b, c and d where RQ = 0.64
(b) Determine the biomass yield coefficient, Yx/s and oxygen yield coefficient Yx/O2

2. Biological denitrification of nitrate-containing waste waters can be described by the following


overall reaction.
NO31 + aCH 3OH + H + bC5 H 7 NO2 + cN 2 + dCO2 + eH 2O
(a) Determine a, b, c, d and e if Yx/s = 0.5 g X / g N
(b) Determine the degrees of reduction for bacteria and methanol

3. Aerobic degradation of an organic compound by mixed culture of organisms in waste water can
be represented by the following equation:
C 3 H 6 O3 + aO2 + bNH 3 c(C 5 H 7 NO 2 ) + dH 2 O + eCO2
(a) Determine a, b, c, d and e if Yx/s = 0.4 g X / g S
(b) Determine the yield coefficients YX/O2 and YX/NH3
(c) Determine the degrees of reduction for the substrate and bacteria and calculate the RQ for
the organism.

4. An aerobic growth of yeast using ethanol as substrate can be described using generalized bioreaction
stoichiometry as
Calculate theoretical yields,

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