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Extraction Yield Calculation Method

The document describes how to calculate the extraction yield of components extracted from canola using different solvents. The extraction yield is measured by extracting 10g of defatted canola sample using 70% methanol, evaporating and freeze-drying the extract, weighing the freeze-dried extract, and calculating the percentage of extract weight compared to the original sample weight. The extraction yield provides information about how efficiently different solvents can extract phenolics from canola.

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216 views1 page

Extraction Yield Calculation Method

The document describes how to calculate the extraction yield of components extracted from canola using different solvents. The extraction yield is measured by extracting 10g of defatted canola sample using 70% methanol, evaporating and freeze-drying the extract, weighing the freeze-dried extract, and calculating the percentage of extract weight compared to the original sample weight. The extraction yield provides information about how efficiently different solvents can extract phenolics from canola.

Uploaded by

rabiekhattab
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOC, PDF, TXT or read online on Scribd

Determination of the Extraction Yield

Determination of the Extraction Yield

The extraction yield is a measure of the solvent efficiency to extract specific components
from the original material. In the case of canola, it will give an idea about the
extractability of phenolics under different solvents.

It could be calculated according to the method of Zhang, Bi, and Liu (2007) as following:

1. Extract 10 g of the defatted canola sample using 70% methanol (you may use
different solvents to compare). The final volume will be 250 ml.
2. Evaporate the extract to dryness by the rotary evaporator under vacuum at 40°C.
3. Lyophilize the dried product in the freeze-dryer to obtain a freeze-dried extract.
4. Weigh the freeze-dried extract.
5. Calculate the extraction yield as the percentage of the weight of the crude extract
to the raw material (10g).
6. Extraction yield (%) = (weight of the freeze-dried extract x 100) / (weight of the
original sample).

Zhang, S., Bi, H., & Liu, C. (2007). Extraction of bio-active components from Rhodiola sachalinensis
under ultrahigh hydrostatic pressure. Separation and Purification Technology, 57, 275−280.

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