Project Proposal
Natalie Hamill Falmouth Marine School FdSc Marine Science 2013
Title
Effects of population density on growth rates and stress inking of juvenile Sepia officinalis. H0: There is no significant difference between population density and growth rates in Sepia Officinalis.
H1: There is a significant difference between population density and growth rates in Sepia Officinalis.
H0: There is no significant difference between population density and the quantity of stress inking in Sepia officinalis. H2: There is a significant difference between population density and the quantity of stress inking in Sepia officinalis.
References
Sykes, A.V et al. (2006). Cuttlefish culture state of the art and future trends. Life and environment, vol. 56 no. 2. pp.129137. Payne, A. G., Agnew, D. J., & Pierce, G. J. (2006). Trends and assessment of cephalopod fisheries. Fisheries Research, vol. 78, no. 1, pp. 1-3. Gilbert, D et al. (1990). Squid as experimental animals. New York: Plenum Press.
Compton, A. and Wiley, L. (2011). Sepia officinalis. Available at: [Link] alis/ (Accessed: 9/2013).
Materials and resources
Tank system Tank x2 Refuge Protein skimmer x2 Canister filter x2 Silicon sand Refractometer pH testing kit Copper test kit Wet weighing Scales Large, measuring bucket Net Camera
Lighting
Piping
Natural marine salt
Associated External Bodies
Dr. James Wood; Cephalopod Specialist.
Roland Anderson; Seattle Aquarium. Roger Villanueva; Calocean Galveston Group; National Resource Centre for Cephalopods Eric Hochberg; Instituto de Cincies del Mar-CMIMA
Greg Casten; Weymouth SeaLife John Rundle; Marine Centre. Biological Association
Prof. Simon Davies; National Marine University of Aquarium and other Plymouth aquaria.
Associated Research and Recommendations
Forsythe, J., Lee, P., Walsh, L., & Clark, T. (2002). The effects of crowding on growth of the European cuttlefish, Sepia officinalis Linnaeus, 1758, reared at two temperatures. Journal of Experimental Marine Biology and Ecology. Vol. 269. No 2: pp. 173185. Domingues, P., Poirier, R., Dickel, L., Almansa, E., Sykes, A., & Andrade, J. P. (2003). Effects of culture density and live prey on growth and survival of juvenile cuttlefish, Sepia officinalis. Aquaculture International. Vol. 11. No 3: pp. 225-242. Correia, M., Domingues, P. M., Sykes, A., & Andrade, J. P. (2005). Effects of culture density on growth and broodstock management of the cuttlefish, Sepia officinalis (Linnaeus, 1758). Aquaculture. Vol. 245. No. 1, pp.163-173.
Methodology and Statistical Analysis
Aquaria Set Up Duration
Densities
Water Chemistry
Feeding Method
Statistical Test
Wet Weighing
Ethical Review
Stress to animals
Human impacts
Weighing method
Contingency Plan
Separate Juveniles
Alternative experiments
Schedule of Operations
Weighing and sampling completed by 13th December Results completed weekly and method shall be written during data collection also. Unpaired t-test shall be used once the results are completed.
Appendix
Risk Assesment
Results Tables
[Link]
Thank you for your time
Any Questions?