B.Tech Sem IV Biotech Practical Guide
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Ashok RathiB.Tech Sem IV Biotech Practical Guide
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Ashok Rathi[Link] SEM IV BIOTECH BSBT 7P INDUSTRIAL MICROBIOLOGY LIST OF PRATICAL 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14.
Sterilization Techniques ( Media , Air , Water ). Construction of various fermenters (Bioreacters ) a note on Chemoautotrophic Bacteria Identification of industrially important microorganism [Link] ,yeasts &bacteria. Production of various products in the lab.- Alcohol, wine , cellulase, protease & bread.. principles of Cheesemaking Isolation of antibiotic producing microorganism from the soil. Penicillin production & testing of antimicrobial activity. acidophillus milk manufacturing. Isolation of streptomysin resistant mutants by replica plating method . Isolation of U.V. Induced auxotrophic mutants. Testing of microbial enzyme activity in the lab. Growth of Staphylococci in milk. Determination of cell growth .
BSBT9P-CELL AND MOLECULAR BIOLOGY LIST OF PRATICAL 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13 14 15. 16. Isolation of genomic DNA fron eukaryotic cells. The applications to Bioinformatics Isolation of RNA from eukaryotic cells . Isolation of proteins from eukaryotic cells a molecular mechanisms are involved in differentiation Isolation of genomic DNA from prokaryotic cells . Isolation of Plasmid DNA from prokaryotic cell. Causes premature chain termination Restriction mapping of plasmid DNA : This exp. Involves single & double digestion of the Plasmid with restriction enzyme . amino acid can be coded by this DNA Gel Electrophoretic separation of DNA & Molecular wt. determination Gel electrophoractic separation of RNA . Gel electrophoratic separation of protein . Transblot analysis of DNA. Gel extraction of DNA. P.C.R. amplification of DNA : Visualisation by gel electrophoresis.