FlyBase Allele Report: Dmel\pum[HMS01685]

General Information

Symbol

Dmel\pum^HMS01685

Species

D. melanogaster

Name

FlyBase ID

FBal0278803

Feature type

allele

Associated gene

Dmel\pum

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01685}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01685}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01685

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ppk.PG

(Lin et al., 2015)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

Phenotype Manifest In

dendrite | third instar larval stage, with Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

larval multidendritic class IV neuron, with Scer\GAL4^ppk.PG

(Lin et al., 2015)

larval multidendritic class IV neuron | third instar larval stage, with Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

Detailed Description

Statement

Reference

Expression of pum^HMS01685 RNAi under the control of Scer\GAL4⁴⁷⁷ results in dendritic field coverage defects and loss of terminal branches in class IV dendritic arborizing neurons in third instar larvae due to reduced branch outgrowth and extensive loss of existing branches during mid-to-late third larval instar.

(Bhogal et al., 2016)

Scer\GAL4^ppk.PG-mediated expression of pum^HMS01685 caused a modest growth deficit in C4da neurons in an otherwise wild-type background.

(Lin et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4⁴⁷⁷, pum^HMS01685 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by hid^A206/hid[+]

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, pum^HMS01685 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Diap1^UAS.cHa, Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^ppk.PG/pum^HMS01685 is a suppressor of abnormal neuroanatomy | larval stage phenotype of path^dg50/Df(3L)BSC773

(Lin et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4⁴⁷⁷, pum^HMS01685 has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible by hid^A206/hid[+]

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, pum^HMS01685 has dendrite | third instar larval stage phenotype, suppressible by hid^A206/hid[+]

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, pum^HMS01685 has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible by Diap1^UAS.cHa, Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

Scer\GAL4⁴⁷⁷, pum^HMS01685 has dendrite | third instar larval stage phenotype, suppressible by Diap1^UAS.cHa, Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^ppk.PG/pum^HMS01685 is a suppressor | partially of larval multidendritic class IV neuron phenotype of path^dg50/Df(3L)BSC773

(Lin et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The dendritic field coverage and branching defects observed in class IV dendritic arborizing neurons of third instar larvae expressing pum^HMS01685 under the control of Scer\GAL4⁴⁷⁷ is suppressed by combination wth a single copy of hid^A206 or by co-expression of Diap1^Scer\UAS.cHa.

(Bhogal et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

38241

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01685}attP40

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

pum^HMS01685

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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