Inshumunta Mahods_d Analysis 8P7oLT
Unil-
Advantages, Disadvantages and Applicabns
AChromdtograbhy "
Vr. Parjanya Shukla
&
Dr. M.P. 3ingh Clases
Advantoqos
)Caskous and Volahilu compounds con b eaily schovatad
and analy20d.
() Bok lualitahve and Quanhtahve onalysis can bs dons
)Tnshrument usud aTimplu, inexpensive and uggad
4)The tachniqu Can b id for xulint anabysis
as operahon otnarumanls and calculahim is simpl
5) suquires Jous omount o sompl (in ul)
So known as micro mehod.
6) Sonsitiviky o tachnique is quita higb
Compounds can be detectad u to porh per million (bbm)
aTh prT bilion (bbA)
[Link] Aratap Sihqh Evin pitogqrama (1o"g9)
) MoT Kon 50 Organic compounds can be onaly2sd
by Ris techni qus
(Raxolvinga potoer is oxhamaly hig
S Combounds tKaB aT dihiaultto onalyzad b
otker tochniques con easily analy2ed GC.
Disadvantaqes
www Dr. Parjanya Shukla
&
() Sampl should bu volailu or vapoTi2ablu. (o Dr. M.P. SunghCaes
complata di'smbubonm b/w M-P. and S.P.
)To vapoviga a comþound 4 neded to be kaate d a
elovatad temprature
so sompb should bi Karmostaba.
(Lorge amount o ga IA ussd as mobil phaso and
Vcan not be uycld.
4) Packing Column i v dfiult
6)
(5) uquva Jong Hnu and operakura oih good knowbdge.
Abblicakons
) QualitahveApayas
xwalitahve anglysis by GC. can be donu by
a) Colloching olutad vopouTs TOm colump
hen ubjchd Ram to apeuic techní qus Jsku
mas Apectroscoby, U.V. 1R NMR et. ,
cb Compaing otenHon im volumu oAambl wih standoyd
(for Column low xate, ogas d temp oq column kubt canstont)
somp and Atondard compounds undar idenkical condi bons
gives Damu stusalubom timu indicahos both compaunds
0TA Aame.
C)Suuontitakv AnalyAiA [Link] Shukla
uanitabva analyain by gas chromaBoqraphy Dr. M.P.3bngh Clases
dapends upon the sige paak
Hioht (H) or Ki avsa d ho peak (A)
4 is Auppoxed tRak K orsa undar tku þaak
is
roporhonal to t tonunabon odutad AubaBanta
Hrao undr cunva o pzak (A) = Hx WV
Gohur H is kight paak
Wy oidh peak a kalf hight
AcSambb) tsomblu)
AGt) C Atd)
whuut
AtAambl) Ptak o6amplu
ar20
AtAhd) Paak o0rea standard)
C tAompla) Comunhabon o Bomple
Cd) Conunabon eAtandatd
usponae actor
PhomouhicaAPblicakons
i) Qualily cmkol and Analysi o dug produck bk-
anhibioHch, Antiviral, onàusthehs , Audabva and hypnoics h
di) Asay d drugs pavity data minabon.
i) In datar mining Ki laval d druga and matabalita
in body luiids ke - plasma, SQYuM , uins at
(iV) TdanHicabon and dakaminabon o falky adds.
) lsolabm and id1nhiicabon o mixturs
o þlant
exacts, carbohydrokes, volahih oilb, amino [Link]
ipids, protins, vitomins, lavowyA, toJauront, er r. M. P. Sngh loas
() MiscaDonuous Applicahons_
0) has chvomaBogvaphy is widaly wad in anaais
tilizs, sulhber, cOAmshCk,panumus, þ[Link]
roduch aod produch o
tor analyAis oday produch Jik milk, buter, chss eh.
) In environmuntal atudies.
(iv) T0r dataehion o staroids tken by ahJakos during
intnabonad sport compahi'tions.
By-Dr. Mohandu Ahatap Sing